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Alterations in circulating cyclic guanosine monophosphate (c-GMP) during short and long ischemia in preconditioning

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Abstract

The aim of this study was to investigate if levels of circulating cyclic guanosine monophosphate (c-GMP) alter, in preconditioning. Twenty-eight rabbits were divided into four groups. In vivo hearts were preconditioned, either with 5 min (group A, n=8) or with 1 min (group B, n=8) ischemia, followed by 10 min reperfusion, while groups C (n=7) and D (n=5) had no interventions. Protection was determined by subjecting groups A, B and C (but not D) to 30 min regional ischemia which was followed (including group D) by 2h reperfusion. Seven blood samples were collected for the assessment of circulating c-GMP at different points of time. All results were expressed in pmol/ml using radioimmunoassay and the infarcted to risk area in percent using fluorescent particles and tetrazolium chloride (TTC). Circulating c-GMP increased during long ischemia only in group A (baseline value 47±4, long ischemic values 60.5±4 and 60.4±4, p<0.05). Circulating c-GMP in group A was significantly higher in the middle of the long ischemia in comparison to the groups B, C and D (60.5±4 vs 43.9±4, 45.8±5 and 43.6±4, p<0.05). Infarcted to risk ratio was lower in group A than in groups B and C (12.2±4 vs 29.6±6 and 34.2±6, respectively, p<0.05). Circulating c-GMP is increased in classically preconditioned in comparison to ineffectively preconditioned hearts or to control groups. This elevation may be related to the protective effect of this phenomenon.

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Authors and Affiliations

  1. 1, Parou str. Ag. Paraskevi, 15343, Athens, Greece

    E. K. Iliodromitis

  2. Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece

    E. K. Iliodromitis, C. C. Papadopoulos, I. A. Paraskevaidis, Z. S. Kyriakides & D. T. Kremastinos

  3. Department of Psychiatry, Eginition University Hospital of Athens, Athens, Greece

    M. Markianos

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  1. E. K. Iliodromitis
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  2. C. C. Papadopoulos
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  3. M. Markianos
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  4. I. A. Paraskevaidis
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  5. Z. S. Kyriakides
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  6. D. T. Kremastinos
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Iliodromitis, E.K., Papadopoulos, C.C., Markianos, M. et al. Alterations in circulating cyclic guanosine monophosphate (c-GMP) during short and long ischemia in preconditioning. Basic Res Cardiol 91, 234–239 (1996). https://doi.org/10.1007/BF00788909

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  • DOI: https://doi.org/10.1007/BF00788909

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