Abstract
The in vitro acetylation of HMG proteins was studied using liver slices of young (18-week) and old (138-week) male rats. Acetylation of total HMG proteins is lower in old age. The incorporation of (14C) acetate into individual HMG proteins varies remarkably with advancing age. Whereas acetylation of high mol. wt. proteins (HMG 1 and 2) is higher, that of low mol. wt. proteins (HMG 14 and 17) is lower in the liver of young rats as compared to the old ones. Spermine stimulates the acetylation of HMG 1 and 14 in young and HMG 1, 2 and 14 in old age. It inhibits the acetylation of HMG 17 in both ages. Dexamethasone decreases the level of incorporation of (14C) into HMG 1 and 17 in young and HMG 14 and 17 in old rats. On the other hand, it stimulates the acetylation of HMG 14 by two-fold in young and that of HMG 1 and 2 by more than three-fold in old rats. Such alteration in the acetylation of HMG proteins may account for age-related changes in the structure and function of chromatin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Wu RS, Panusz HT, Hatch CL & Bonner WM (1986) CRC Rev. Biochem. 20:201–263
Thakur MK (1983) Arch. Gerontol. Geriatr. 2:1–10
Pina B, Martinez P & Suau P (1988) Eur. J. Biochem. 174:311–315
Bianchi ME, Beltrame M & Paonessa G (1989) Science 243:1056–1059
Alexandrova AE & Beltchev BG (1988) Biochem. Biophys. Res. Commun. 154:918–927
Tremethick DJ & Molloy PL (1989) FEBS Lett. 242: 346–350
Brotherton TW & Ginder GD (1986) Biochemistry 25: 3447–3454
Dorbic T & Wittig B (1987) EMBO J. 6:2393–2399
Einck L & Bustin M (1985) Exp. Cell Res. 156:295–310
Janne J, Poso H & Rain A (1978) Biochim. Biophys. Acta 473:241–253
Cooper E & Spaulding SW (1985) Molec. Cell. Endocrinol. 39:1–20
Prasad S & Thakur MK (1988) Biochem. Internat. 16: 375–382
Goodwin GH, Rabbani A, Nicolas RH & Johns EW (1977) FEBS Lett. 80:413–416
Thakur MK & Prasad S (1989) Mut. Res. 219:107–111
Bucci LR, Brock WA, Goldknopf IL & Meistrich ML (1984) J. Biol. Chem. 259:8840–8846
Saxholm HJK, Pestana A, O'Conner L, Sattler CA & Pitot CA (1982) Molec. Cell. Biochem. 46:129–153
Böhm J, Schlaeger EJ & Knippers R (1980) Eur. J. Biochem. 112:353–382
Kanungo MS (1980) In: Biochemistry of Aging (pp 18–78). Academic Press, London
Thakur MK (1984) Mech. Age. Dev. 27:263–286
Reeves R & Candido EPM (1980) Nucleic Acids Res. 8: 1947–1963
Dod B, Kervabon A & Parello J (1982) Eur. J. Biochem. 121:401–405
Vu QA, Zhang D, Chroners ZC & Nelson DA (1987) FEBS Lett. 220:79–83
Bose R & Kanungo MS (1982) Arch. Gerontol. Geriatr. 1:339–348
Sen D & Crothers DM (1986) Biochemistry 25: 1495–1503
Liew CC, Suria D & Gornall AG (1973) Endocrinol. 93: 1025–1034
Prasad S & Thakur MK (1987) Molec. Biol. Rep. 12: 273–276
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Prasad, S., Thakur, M.K. In vitro acetylation of the liver HMG non-histone proteins and its modulation by spermine and dexamethasone during aging of rats. Mol Biol Rep 13, 221–224 (1988). https://doi.org/10.1007/BF00788174
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00788174