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Interaction of histone H1 with superhelical DNA conformational studies and influence of ionic strength

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Abstract

The interaction of histone H1 with superhelical SV40 DNA at low ionic strength (≈0.02 M NaCl) results in the formation of DNP double-fibers and bundle-and cablelike twisted side-by-side associates of several of these double-fibers. On the basis of simple cylindrical or ellipsoidal models the sedimentation properties of these structures can be calculated in accordance with the experiment allowing a direct assignment of electron microscopical and hydrodynamic results. Sedimentation measurements in dependence on the ionic strength indicate a redistribution of H1 resulting in the formation of associates at 0.04 M NaCl and of aggregates at higher salt concentration. Double-fibers are present up to physiological salt concentrations.

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Authors and Affiliations

  1. Zentralinstitut für Molekularbiologie der Akademie der Wissenschaften der DDR, DDR-1115, Berlin-Buch, GDR

    M. Böttger, C. -U. von Mickwitz, S. Scherneck & R. Lindigkeit

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  1. M. Böttger
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  2. C. -U. von Mickwitz
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  3. S. Scherneck
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  4. R. Lindigkeit
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Böttger, M., von Mickwitz, C.U., Scherneck, S. et al. Interaction of histone H1 with superhelical DNA conformational studies and influence of ionic strength. Mol Biol Rep 10, 3–8 (1984). https://doi.org/10.1007/BF00775146

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  • DOI: https://doi.org/10.1007/BF00775146

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