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Electrophysiology of the inner mitochondrial membrane

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Abstract

The application of electrophysiological techniques to mitochondrial membranes has allowed the observation and partial characterization of several ion channels, including an ATP-sensitive K+-selective one, a high-conductance “megachannel”, a 107 pS anionic channel and three others studied at alkaline pH's. A reliable correlation with the results of non-electrophysiological studies has been obtained so far only for the first two cases. Activities presumed to be associated with the Ca2+ uniporter and with the adenine nucleotide translocator, as well as the presence of various other conductances have also been reported. The review summarizes the main properties of these pores and their possible relationship to permeation pathways identified in biochemical studies.

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References

  • Alexander, B. E. E., and Klotz, U. (1993).Pharmazie 48 563–570.

    Google Scholar 

  • Antonenko, Yu. N., Kinnally, K. W., and Tedeschi, H. (1991a).J. Membr. Biol. 124 151–158.

    Google Scholar 

  • Antonenko, Yu. N., Kinnally, K. W., Perini, S., and Tedeschi, H. (1991b).FEBS Lett. 285 89–93.

    Google Scholar 

  • Beavis, A. D. (1989).J. Biol. Chem. 264 1508–1515.

    Google Scholar 

  • Beavis, A. D. (1992).J. Bioenerg. Biomembr. 24 77–90.

    Google Scholar 

  • Beavis, A. D., and Garlid, K. D. (1987).J. Biol. Chem. 262 15085–15093.

    Google Scholar 

  • Beavis, A. D., and Garlid, K. D. (1988).J. Biol. Chem. 263 7574–7580.

    Google Scholar 

  • Beavis, A. D., and Powers, M. F. (1989),J. Biol. Chem. 264 17148–17155.

    Google Scholar 

  • Beavis, A. D., Lu, Y., and Garlid, K. D. (1993).J. Biol. Chem. 268 997–1004.

    Google Scholar 

  • Bernardi, P. (1992).J. Biol. Chem. 267 8834–8839.

    Google Scholar 

  • Bernardi, P., Angrilli, A., Ambrosin, V., and Azzone, G. F. (1989).J. Biol. Chem. 264 18902–18906.

    Google Scholar 

  • Bernardi, P., Angrilli, A., and Azzone, G. F. (1990).Eur. J. Biochem. 188 91–97.

    Google Scholar 

  • Bernardi, P., Broekemeier, K. M. and Pfeiffer, D. R. (1994). Minireview in this issue.

  • Bernardi, P., Vassanelli, S., Veronese, P., Colonna, R., Szabó, I., and Zoratti, M. (1992).J. Biol. Chem. 267 2934–2939.

    Google Scholar 

  • Bragadin, M., Pozzan, T., and Azzone, G. F. (1979).Biochemistry 18 5972–5978.

    Google Scholar 

  • Brdiczka, D. (1991).Biochim. Biophys. Acta 1071 291–312.

    Google Scholar 

  • Brierly, G. P., Jurkowitz, M., and Jung, D. W. (1978).Arch. Biochem. Biophys. 190 181–192.

    Google Scholar 

  • Brokemeier, K. M., Dempsey, M. E., and Pfeiffer, D. R. (1989)J. Biol. Chem. 264 7826–7830.

    Google Scholar 

  • Campo, M. L., Kinnally, K. W., and Tedeschi, H. (1992).J. Biol. Chem. 267 8123–8127.

    Google Scholar 

  • Campo, M. L., Kinnally, K. W., and Tedeschi, H. (1994).Biophys. J. 66, A22.

    Google Scholar 

  • Chich, J.-F., Goldschmidt, D., Thieffry, M., and Henry, J.-P. (1991).Eur. J. Biochem. 196 29–35.

    Google Scholar 

  • Colombini, M. (1989).J. Membr. Biol. 111 103–111.

    Google Scholar 

  • Costa, G., Kinnally, K. W., and Diwan, J. J. (1991).Biochem. Biophys. Res. Commun. 175 305–310 (1991).

    Google Scholar 

  • Crompton, M., and Costi, A. (1988).Eur. J. Biochem. 178 488–501.

    Google Scholar 

  • Crompton, M. Ellinger, H., and Costi, A. (1988).Biochem. J. 255 357–360.

    Google Scholar 

  • Davidson, A. M., and Halestrap, A. P. (1990).Biochem. J. 268 147–152.

    Google Scholar 

  • Dierks, T., Salentin, A., Heberger, C., and Kraemer, R. (1990a).Biochim. Biophys. Acta 1028 268–280.

    Google Scholar 

  • Dierks, T., Salentin, A., and Kraemer, R. (1990b).Biochim. Biophys. Acta 1028 281–288.

    Google Scholar 

  • Diwan, J. J., Paliwal, R., Kaftan, E., and Bawa, R. (1990).FEBS Lett. 273 215–218.

    Google Scholar 

  • Févre, F., Chich, J.-F., Lauquin, G. J. M., Henry, J.-P., and Thieffry, M. (1990).FEBS Lett. 262 201–204.

    Google Scholar 

  • Févre, F., Henry, J.-P., and Thieffry, M. (1993).J. Bioenerg. Biomembr. 25 55–60.

    Google Scholar 

  • Févre, F., Henry, J.-P. and Thieffry, M. (1994)Biophys. J. 66 1887–1894.

    Google Scholar 

  • Garlid, K. D., and Beavis, A. D. (1986).Biochim. Biophys. Acta 853 187–204.

    Google Scholar 

  • Gunter, T. E., and Pfeiffer, D. R. (1990).Am. J. Physiol. 258 C755-C786.

    Google Scholar 

  • Halestrap, A. P. (1989).Biochim. Biophys. Acta 973 355–382.

    Google Scholar 

  • Halestrap, A. P. (1991).Biochem. J. 278 715–719.

    Google Scholar 

  • Halestrap, A. P., Quinlan, P. T., Whipps, D. E., and Armston, A. E. (1986).Biochem. J. 236 779–787.

    Google Scholar 

  • Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J. (1981).Pfluegers Arch. 391 85–100.

    Google Scholar 

  • Haworth, R. A. and Hunter, D. R. (1979).Arch. Biochem. Biophys. 195 460–467.

    Google Scholar 

  • Haworth, R. A., and Hunter, D. R. (1980).J. Membr. Biol. 54 231–236.

    Google Scholar 

  • Hayman, K. A., and Ashley, R. H. (1993).J. Membr. Biol. 136 191–197.

    Google Scholar 

  • Hayman, K. A., Spurway, T. D., and Ashley, R. H. (1993).J. Membr. Biol. 136 181–190.

    Google Scholar 

  • Henry, J.-P., Chich, J.-F., Goldschmidt, D., and Thieffry, M. (1989).J. Membr. Biol. 112 139–147.

    Google Scholar 

  • Hunter, D. R. and Haworth, R. A. (1979).Arch. Biochem. Biophys. 195 453–459.

    Google Scholar 

  • Inoue, I., Nagase, H., Kishi, K., and Higuti, T. (1991).Nature (London) 352 244–247.

    Google Scholar 

  • Jezek, P., and Garlid, K. D. (1990).J. Biol. Chem. 265 19303–19311.

    Google Scholar 

  • Jezek, P., Orosz, D. E., and Garlid, K. D. (1990).J. Biol. Chem. 265 19296–19302.

    Google Scholar 

  • Kapus, A., Szaszi, K., Kaldi, K., Ligeti, E., and Fonyo, A. (1990).J. Biol. Chem. 265 18063–18066.

    Google Scholar 

  • Kinnally, K. W., Campo, M. L., and Tedeschi, H. T. (1989).J. Bioenerg. Biomembr. 21 497–506.

    Google Scholar 

  • Kinnally, K. W., Zorov, D. B., Antonenko, Yu. N., and Perini, S. (1991).Biochem. Biophys. Res. Commun. 176 1183–1188.

    Google Scholar 

  • Kinnally, K. W., Antonenko, Yu. N., and Zorov, D. B. (1992).J. Bioenerg. Biomembr. 24 99–110.

    Google Scholar 

  • Kinnally, K. W., Zorov, D. B., Antonenko, Yu. N., Snyder, A. H., McEnery, M. W., and Tedeschi, H. (1993).Proc. Natl. Acad. Sci. USA 90 1374–1378.

    Google Scholar 

  • Klingenberg, M. (1981).Nature (London) 290 449–454.

    Google Scholar 

  • Klingenberg, M. (1990).Trends Biochem. Sci. 15 108–112.

    Google Scholar 

  • Klingenberg, M. (1993).J. Bioenerg. Biomembr. 25 447–457.

    Google Scholar 

  • Klitch, T., and Siemen, D. (1991).J. Membr. Biol. 122 69–75.

    Google Scholar 

  • Krause, J., Hay, R., Kowollik, C., and Brdiczka, D. (1986).Biochim. Biophys. Acta 860 690–698.

    Google Scholar 

  • Langer, S. Z., Arbilla, S., Tan, S., Lloyd, P. G., Allen, J., and Wick, A. E. (1990).Pharmacopsychiatry 23 103–107.

    Google Scholar 

  • LeQuoc, K., and LeQuoc, D. (1985).J. Biol. Chem. 260 7422–7428.

    Google Scholar 

  • Mannella, C. (1992).Trends Biochem. Sci. 17 315–320.

    Google Scholar 

  • Mannella, C., Forte, M., and Colombini, M. (1992).J. Bioenerg. Biomembr. 24 7–19.

    Google Scholar 

  • Manon, S., and Guerin, M. (1993).J. Bioenerg. Biomembr. 25 671–678.

    Google Scholar 

  • Massari, S., and Azzone, G. F. (1972).Biochim. Biophys. Acta 283 23–29.

    Google Scholar 

  • McEnery, M. W., Snowman, A. M., Trifiletti, R. R., and Snyder, S. H. (1992). Proc. Natl. Acad. Sci. USA89 3170–3174.

    Google Scholar 

  • McEnery, M. W. (1992).J. Bioenerg. Biomembr. 24 63–69.

    Google Scholar 

  • McGuinness, O., Yafei, N., Costi, A., and Crompton, M. (1990).Eur. J. Biochem. 194 671–679.

    Google Scholar 

  • Mironova, G. D., Fedotcheva, N. I., Makarov, P. R., Pronevich, L. A., and Mironov, G. P. (1981).Biophysics 26 458–465.

    Google Scholar 

  • Mironova, G., Sirota, T. V., Pronevich, L. A., Trofimenko, N. V., Mironov, G. P., Grigorjev, P. A., and Kondrashova, M. N. (1982).J. Bioenerg. Biomembr. 14 213–225.

    Google Scholar 

  • Mironova, G. D., Baumann, M., Kolomylikin, O., Krasichkova, Z., Berdimuratov, A., Sirota, T., Virtanen, I., and Saris, N. L. (1994).J. Bioenerg. Biomembr. 26 231–238.

    Google Scholar 

  • Mirzabekov, T., Ballarin, C., Nicolini, M., Zatta, P., and Sorgato, M. (1993).J. Membr. Biol. 133 129–143.

    Google Scholar 

  • Moran, O., Sandri, G., Panfili, E., Stuehmer, W., and Sorgato, M. C. (1990).J. Biol. Chem. 265 908–913.

    Google Scholar 

  • Moran, O., and Sorgato, M. C. (1992).J. Bioenerg. Biomembr. 24 91–98.

    Google Scholar 

  • Munn, E. A. (1974).The Structure of Mitochondria, Academic Press, London/New York.

    Google Scholar 

  • Nicholls, D. G. (1974).Eur. J. Biochem. 49 585–593.

    Google Scholar 

  • Nicholls, D. G. (1979).Biochim. Biophys. Acta 549 1–29.

    Google Scholar 

  • Nicolli, A., Redetti, A., and Bernardi, P. (1991).J. Biol. Chem. 266 9465–9470.

    Google Scholar 

  • Nicolli, A., Petronilli, V., and Bernardi, P. (1993).Biochemistry 32 4461–4465.

    Google Scholar 

  • Paliwal, R., Costa, G., and Diwan, J. J. (1992).Biochemistry 31 2223–2229.

    Google Scholar 

  • Paucek, P., Mironova, G., Mahdi, F., Beavis, A. D., Woldegiorgis, G., and Garlid, K. D. (1992).J. Biol. Chem. 267 26062–26069.

    Google Scholar 

  • Petronilli, V., Szabó, I., and Zoratti, M. (1989).FEBS Lett. 259 137–143.

    Google Scholar 

  • Petronilli, V., Cola, C., and Bernadi, P. (1993).J. Biol. Chem. 268 1011–1016.

    Google Scholar 

  • Powers, M. F., and Beavis, A. D. (1991).J. Biol. Chem. 266 17250–17256.

    Google Scholar 

  • Reed, K. C., and Bygrave, F. L. (1974).Biochem. J. 140 143–155.

    Google Scholar 

  • Sakmann, B., and Neher, E. (1983).Single-Channel Recording, Plenum Press, New York/London.

    Google Scholar 

  • Saris, N.-E., Sirota, T. V., Virtanen, I., Niva, K., Penttila, T., Dogachova, L. P., and Mironova, G. (1993).J. Bioenerg. Biomembr. 25 307–311.

    Google Scholar 

  • Selwyn, M. J., and Walker, H. A. (1977).Biochem. J. 166 137–139.

    Google Scholar 

  • Selwyn, M. J., Dawson, A. P., and Fulton, D. V. (1979).Biochem. Soc. Trans. 7 216–219.

    Google Scholar 

  • Selwyn, M. J., Ng, C. L. T., and Choo, H. L. (1990).FEBS Lett. 260 205–208.

    Google Scholar 

  • Snyder, S., Verma, A., and Trifiletti, R. R. (1987).FASEB J. 1 282–288.

    Google Scholar 

  • Sorgato, M. C., and Moran, O. (1993).Crit. Rev. Biochem. Mol. Biol. 18 127–171.

    Google Scholar 

  • Sorgato, M. C., Keller, B. U. and Stuehmer, W. (1987)Nature (London) 330 498–500.

    Google Scholar 

  • Sorgato, M. C., Lippe, G., Keller, B. U. and Stuehmer, W. (1988). InIntegration of Mitochondrial Function (Lemasters, J. J., Hackenbrock, C. R., Thurman, R. G., and Westerhoff, H. V., eds.), Plenum Press, New York, pp. 305–311.

    Google Scholar 

  • Sorgato, M. C. Moran, O., DePinto, V., Keller, B. U., and Stuehmer, W. (1989).J. Bioenerg. Biomembr. 21 485–506.

    Google Scholar 

  • Szabó, I., and Zoratti, M. (1991).J. Biol. Chem. 266 3376–3379.

    Google Scholar 

  • Szabó, I., and Zoratti, M. (1992).J. Bioenerg. Biomembr. 24 111–117.

    Google Scholar 

  • Szabó, I., and Zoratti, M. (1993).FEBS Let. 330 201–205.

    Google Scholar 

  • Szabó, I., Bernardi, P., and Zoratti, M. (1992).J. Biol. Chem. 267 2940–2946.

    Google Scholar 

  • Szabó, I., DePinto, V., and Zoratti, M. (1993a).Biophys. J. 64, A342.

  • Szabó, I., DePinto, V., and Zoratti, M. (1993b).FEBS Lett. 330 206–210.

    Google Scholar 

  • Thieffry, M., Chich, J.-F., Goldschmidt, D., and Henry, J.-P. (1988).EMBO J. 7 1449–1454.

    Google Scholar 

  • Thieffry, M., Neyton, J., Pelleschi, M., Fevre, F., and Henry, J.-P. (1992).Biophys. J. 63 333–339.

    Google Scholar 

  • Tikhonova, I. M., Andreyev, A. Yu., Antonenko, Yu. N., Kaulen, A. D., Komrakov, A. Yu., and Skulachev, V. P. (1994).FEBS Lett. 337 231–234.

    Google Scholar 

  • Vallette, F. M., Juin, P., Pelleschi, M. and Henry, J.-P. (1994)J. Biol. Chem. 269 13367–13374.

    Google Scholar 

  • Verma, A., and Snyder, S. H. (1988).Mol. Pharmacol. 34 800–805.

    Google Scholar 

  • Wehrle, J. P., Jurkowitz, M., Scott, K. M., and Brierley, G. P. (1976).Arch. Biochem. Biophys. 174 312–323.

    Google Scholar 

  • Wunder, U. R., and Colombini, M. (1991).J. Membr. Biol. 123 83–91.

    Google Scholar 

  • Zizi, M., Forte, M., Blachly-Dyson, E., and Colombini, M. (1994).J. Biol. Chem. 269 1614–1616.

    Google Scholar 

  • Zoratti, M., Szabó, I., and DePinto, V. (1992). InMolecular Biology of Mitochondrial Transport Systems (Colombini, M., and Forte, M., eds.), NATO ASI Series, Vol. H83, Springer Verlag, Heidelberg, pp. 153–168.

    Google Scholar 

  • Zoratti, M., Szabó, I., Bathori, G., Starc, T., Wolff, D., and Schatz, G. (1994).Biophys. J. 66, A22.

    Google Scholar 

  • Zorov, D. B., Kinnally, K. W., Perini, S., and Tedeschi, H. (1992a).Biochim. Biophys. Acta 1105 263–270.

    Google Scholar 

  • Zorov, D. B., Kinnally, K. W., and Tedeschi, H. (1992b).J. Bioenerg. Biomembr. 24 119–124.

    Google Scholar 

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  1. CNR Unit for the Physiology of Mitochondria, Department of Biomedical Sciences, University of Padova, Padova, Italy

    Mario Zoratti & Ildikó Szabó

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Zoratti, M., Szabó, I. Electrophysiology of the inner mitochondrial membrane. J Bioenerg Biomembr 26, 543–553 (1994). https://doi.org/10.1007/BF00762739

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