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Proton transfer and energy coupling in the bacteriorhodopsin photocycle

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Abstract

A description of the rate constants and the energetics of the elementary reaction steps of the photocycle of bacteriorhodopsin has been helpful in understanding the mechanism of proton transport in this light-driven pump. The evidence suggests a single unbranched reaction sequence, BR-hv → K ↔ L ↔ M1 → M2 ↔ O → BR, where coupling to the protonmotive force is at the energetically and mechanistically important M1 → M2 step. The consequences of site-specific mutations expressed homologously inHalobacterium halobium have revealed characteristics of the Schiff base deprotonation in the L → M1 reaction, the reorientation of the Schiff base from the extracellular to the cytoplasmic side in the M1 → M2 reaction, and the reprotonation of the Schiff base in the M2 → N reaction.

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  1. Department of Physiology and Biophysics, University of California, 92717, Irvine, California

    Janos K. Lanyi

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Lanyi, J.K. Proton transfer and energy coupling in the bacteriorhodopsin photocycle. J Bioenerg Biomembr 24, 169–179 (1992). https://doi.org/10.1007/BF00762675

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