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Derivation and characterization of a zebrafish liver cell line

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Abstract

ZF-L cells were derived from normal adult zebrafish liver, and have been growing in culture for more than 100 generations. The cells were derived in basal nutrient medium supplemented with fetal bovine serum (FBS), trout serum, trout embryo extract, bovine insulin and mouse epidermal growth factor. After 50 generations in culture, optimal growth of the cells was achieved in medium supplemented with FBS (5%) and trout serum (0.5%). ZF-L cells were hypodiploid (modal chromosome number= 46) and exhibited an epithelial morphology. ZF-L cell homogenates exhibited alanine and aspartate aminotransferase, glucose-6-phosphatase and alkaline phosphatase enzyme activities. The cells synthesized and released several proteins into the culture medium, including a 70 kDa protein recognized by anti-bovine serum albumin IgG.

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Abbreviations

βNF:

β-naphthoflavone

EGF:

epidermal growth factor

EROD:

7-ethoxyresorufinO-deethylase

FBS:

fetal bovine serum

PBS:

phosphate-buffered saline

PMSF:

phenylmethylsulfonyl fluoride

TCDD:

2,3,7,8-tetrachlorodibenzo-p-dioxin

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Authors and Affiliations

  1. Department of Animal Sciences, Purdue University, 47907, West Lafayette, IN, U.S.A.

    C. Ghosh, Y. L. Zhou & P. Collodi Ph.D.

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  1. C. Ghosh
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  2. Y. L. Zhou
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  3. P. Collodi Ph.D.
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Ghosh, C., Zhou, Y.L. & Collodi, P. Derivation and characterization of a zebrafish liver cell line. Cell Biol Toxicol 10, 167–176 (1994). https://doi.org/10.1007/BF00757560

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