Abstract
The concept that creatine phosphokinase is bound to the outer surface of the heart mitochondrial inner membrane originated from observations that the enzyme is retained by water-swollen heart mitochondria and by digitonintreated heart mitochondria suspended in isotonic sucrose. The present study establishes that digitonin-treated mitochondria release creatine phosphokinase in isotonic KCl, and other investigators have reported an identical response for the water-swollen organelles. These observations suggest that mitochondrial creatine phosphokinase is not bound to the outer surface of the inner membrane at a site adjacent to the adenine nucleotide translocase under physiologic conditions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altschuld, R. A., and Brierley, G. P. (1977).J. Mol. Cell. Cardiol. 7, 875.
Barbour, R. L., Ribaudo, J., and Chan, S. H. P. (1984).J. Biol. Chem. 259, 8246.
Bittl, J. A., Weisfeldt, M. L., and Jacobus, W. E. (1985).J. Biol Chem. 260, 208.
Borrebaek, B. (1980),Arch. Biochem. Biophys. 203, 827.
Farrell, E. C., Baba, N., Brierley, G. P., and Grumer, H. D. (1972).Lab. Invest. 27, 209.
Font, B., Vial, C., Goldschmidt, D., Eichenberger, D., and Gautheron, D. C. (1981).Arch. Biochem. Biophys. 212, 195.
Font, B., Vial, C., Goldschmidt, D., Eichenberger, D., and Gautheron, D. C. (1981).Arch. Biochem. Biophys. 220, 541.
Gellerich, F., and Saks, V. A. (1982).Biochem. Biophys. Res. Commun. 105, 1473.
Hall, N., and DeLuca, M. (1980).Arch. Biochem. Biophys. 201, 674.
Hall, N., Addis, P., and DeLuca, M. (1979).Biochemistry 18, 1745.
Jacobus, W. E., and Lehninger, A. L. (1973).J. Biol. Chem. 248, 4803.
Jung, D. W., Chavez, E., and Brierley, G. P. (1977).Arch. Biochem. Biophys. 183, 452.
Klingenberg, M., and Pfaff, E. (1966). InRegulation of Metabolic Processes in Mitochondria (Tager, J. M., Papa, S., Quagliariello, E., and Slater, E. C., eds.), BBA Library, Vol. 7, Elsevier, Amsterdam, pp. 180–201.
Lipskaya, T. Y., Temple, V. D., Belousova, L. V., and Molokova, E. V. (1980a).Biokhimiya 45, 1347.
Lipskaya, T. Y., Temple, V. D., Belousova, L. V., Molokova, E. V., and Rybina, I. V. (1980b)Biokhimiya 45, 1155.
Lipskaya, T. Y., Temple, V. D., Belousova, L. V., Molokova, E. V., and Rybina, I. V. (1982).Adv. Myocardiol. 3, 597.
Lott, J. A., and Turner, K. (1973).Am. J. Clin. Pathol. 59, 846.
Meyer, R. A., Sweeney, H. L., and Kushmerick, M. J. (1984).Am. J. Physiol. 246, C365.
Müller, M., Moser, R., Cheneval, D., and Carafoli, E. (1985).J. Biol. Chem. 260, 3839.
Murphy, M. P., Hohl, C. M., Brierley, G. P., and Altschuld, R. A. (1982).Circ. Res. 541, 560.
Pette, D. (1966). InRegulation of Metabolic Processes in Mitochondria (Tager, J. M., Papa, S., Quagliariello, E., and Slater, E. C., eds.), BBA Library, Vol. 7, Elsevier, Amsterdam, pp. 28–79.
Saks, V. A., Lipina, N. V., Smirnov, V. N., and Chazov, E. I. (1976).Arch. Biochem. Biophys. 173, 34.
Schnaitman, C., and Greenawalt, J. W. (1968).J. Cell. Biol. 38, 158.
Scholte, H. R., Weijers, P. J., and Wit-Peeters, E. M. (1973).Biochim. Biophys. Acta 291, 764.
Scott, K. M., Knight, V. A., Settlemire, C. T., and Brierley, G. P. (1970).Biochemistry 9, 714.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wenger, W.C., Murphy, M.P., Brierley, G.P. et al. Effects of ionic strength and sulfhydryl reagents on the binding of creatine phosphokinase to heart mitochondrial inner membranes. J Bioenerg Biomembr 17, 295–303 (1985). https://doi.org/10.1007/BF00751106
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00751106