Abstract
The following characteristics are reported for mitochondrial ATPase prepared by the chloroform extraction method: (1) The pH optimum for enzyme activity is at 8.0. (2) The neutral anesthetic benzocaine inhibits the enzyme at all pH values. (3) Reciprocal plots of 1/v versus 1/[ATP] show that inhibition by lidocaine, tetracaine, dibucaine, and chlorpromazine is noncompetitive; slope and intercept replots are hyperbolic, showing that the inhibition is partial rather than complete.
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Beechey, R. B., Hubbard, S. A., Linnett, P. E., Mitchell, A. D., and Munn, E. A. (1975).Biochem. J. 148 533–537.
Chang, T., and Penefsky, H. S. (1973).J. Biol. Chem. 248 2746–2754.
Chazotte, B. (1981). Ph. D. Thesis, Northern Illinois University, DeKalb, Illinois.
Chazotte, B., and Vanderkooi, G. (1981).Biochim. Biophys. Acta 636 153–161.
Chazotte, B., Vanderkooi, G., and Chignell, D. (1982).Biochim. Biophys. Acta 680 310–316.
Cleland, W. W. (1970). InThe Enzymes (Boyer, P. D., ed.), Vol. 2, 3rd edn., Academic Press, New York, pp. 1–65.
Ebel, R. E., and Lardy, H. A. (1975).J. Biol. Chem. 250 4992–4995.
Fisher, R. J., Liang, A. M., and Sundstrom, G. C. (1981).J. Biol. Chem. 256 707–715.
Gregg, C. T. (1967).Methods Enzymol. 10 181–185.
Laikind, P. K., and Allison, W. S. (1983).J. Biol. Chem. 258 11700–11704.
Laikind, P. K., Goldenberg, T. M., and Allison, W. S. (1982).Biochem. Biophys. Res. Commun. 109 423–427.
Lenaz, G., Curatola, G., Mazzanti, L., Parenti-Castelli, G., and Bertoli, E. (1978).Biochem. Pharmacol. 27 2835–2844.
Linnett, P. E., Mitchell, A. D., Partis, M. D., and Beechey, R. B. (1979).Methods Enzymol. 55 337–343.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951).J. Biol. Chem. 193 265–275.
Montecucco, C., Ballardin, S., Zaccolin, G. P., and Pozzan, T. (1981).Biochem. Pharmacol. 30 2989–2992.
Palatini, P. (1979). InRecent Advances in Receptor Chemistry (Gualtieri, F., Giannella, M., and Melchiorre, C., eds.), Elsevier, Amsterdam, pp. 111–126.
Palatini, P. (1982).Mol. Pharmacol. 21 415–421.
Penefsky, H. S., Pullman, M. E., Datta, A., and Racker, E. (1960).J. Biol. Chem. 235 3330–3336.
Pullman, M. E., Penefsky, H. S., Datta, A., and Racker, E. (1960).J. Biol. Chem. 235 3322–3329.
Ritchie, J. M., and Ritchie, B. R. (1968).Science 162 1394–1395.
Singh, J. P., Babcock, D. F., and Lardy, H. A. (1983).Arch. Biochem. Biophys. 221 293–303.
Ulrich, F. (1964).J. Biol. Chem. 239 3532–3536.
Vanderkooi, G., Chazotte, B., and Biethman, R. (1978).FEBS Lett. 90 21–23.
Vanderkooi, G., Shaw, J., Storms, C., Vennerstrom, R., and Chignell, D. (1981).Biochim. Biophys. Acta 635 200–203.
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Adade, A.B., Chignell, D. & Vanderkooi, G. Local anesthetics: A new class of partial inhibitors of mitochondrial ATPase. J Bioenerg Biomembr 16, 353–363 (1984). https://doi.org/10.1007/BF00743231
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DOI: https://doi.org/10.1007/BF00743231