Abstract
Photosystem II particles have been prepared from spinach andChlamydomonas reinhardii CW15 thylakoids. Photosynthetic electron transport in these particles is inhibited by phenolic compounds like dinoseb, but not by atrazine and diuron. The labeling patterns obtained by photoaffinity labels derived from either atrazine (azido-atrazine) or the phenolic herbicide dinoseb (azidodinoseb) were compared in photosystem II particles and thylakoids. Whereas azido-atrazine in thylakoids of spinach as well as ofChlamydomonas labels a 32-kilodalton peptide, this label does not react in photosystem II particle preparations. Azido-dinoseb, however, labels both the thylakoid membranes and the particles, predominantly polypeptides in the 40–53 kilodalton molecular weight region. Since the latter polypeptides are probably part of the reaction center of photosystem II, it is suggested that phenolic compounds have their inhibition site within the reaction center complex. This indicates that the atrazine-binding 32-kilodalton peptide is either absent or functionally inactive in photosystem II particles, whereas the phenol inhibitor-binding peptides are not.
Similar content being viewed by others
References
Amrhein, N., and Filner, P. (1973).Proc. Natl. Acad. Sci. USA 70 1099–1103.
Arnon, D. J. (1949).Plant Physiol 24 1–15.
Astier, C., and Joset-Espardellier, F. (1981).FEBS Lett. 129 47–51.
Bayley, H., and Knowles, J. R. (1977).Methods Enzymol. 46 69–114.
Berrer, D., and Vogel, C. (1970). Ger. Pat., Offenlegungs-Schrift 1914012;Chem. Abstr. 72 43743.
Böger, P., and Kunert, K.-J. (1979).Z. Naturforsch. Teil C 34 1015–1020.
Bouges-Bocquet, B. (1973).Biochim. Biophys. Acta 314 250–256.
Chua, N.-H. (1980).Methods Enzymol. 69C 434–446.
Chua, N.-H., and Blomberg, F. (1979).J. Biol. Chem. 254 215–223.
Chua, N.-H., and Bennoun, P. (1975).Proc. Natl. Acad. Sci. USA 72 2175–2179.
Croze, E., Kelly, M., and Horton, P. (1979).FEBS Lett. 103 22–26.
Davies, D. R., and Plaskitt, A. (1971).Genet. Res. 17 33–43.
Delepelaire, P., and Chua, N.-H. (1979).Proc. Natl. Acad. Sci. USA 76 111–115.
Diner, B. A., and Wollman, F.-A. (1980).Eur. J. Biochem. 110 521–526.
Gardner, G. (1981).Science 211 937–940.
Girard, J., Chua, N.-H., Bennoun, P., Schmidt, G., and Delosme, M. (1980).Curr. Genet. 2 215–221.
Gressel, J. (1982).Plant Sci. Lett. 25 99–106.
Jansson, C., Andersson, B., and Åkerlund, H.-E. (1979).FEBS Lett. 105 177–180.
Kamienietzky, A., and Nelson, N. (1975).Plant Physiol. 55 282–287.
Koenig, F., and Vernon, L. P. (1981).Z. Naturforsch. Teil C 36 295–304.
Machold, O., Simpson, D. J., and Møller, B. L. (1979).Carlsberg Res. Commun. 44 235–254.
Mattoo, A. K., Pick, U., Hoffmann-Falk, H., and Edelman, M. (1981).Proc. Natl. Acad. Sci. USA 78 1572–1576.
Mullet, J. E., and Arntzen, C. J. (1981).Biochim. Biophys. Acta 635 236–248.
Oettmeier, W., and Masson, K. (1980).Pestic. Biochem. Physiol. 14 86–97.
Oettmeier, W., Masson, K., and Johanningmeier, U. (1980).FEBS Lett. 118 267–270.
Oettmeier, W., Masson, K., and Johanningmeier, U. (1982a).Biochim. Biophys. Acta 679 376–383.
Oettmeier, W., Masson, K., Fedtke, C., Konze, J., and Schmidt, R. (1982b).Pestic. Biochem. Physiol., in press.
Pfister, K., and Arntzen, C. J. (1979).Z. Naturforsch. Teil C 34 996–1009.
Pfister, K., Steinback, K. E., Gardner, G., and Arntzen, C. J. (1981).Proc. Natl. Acad. Sci. USA 78 981–985.
Regitz, G., and Ohad, J. (1976).J. Biol. Chem. 251 247–252.
Reimer, S., Link, K., and Trebst, A. (1979).Z. Naturforsch. Teil C 34 419–426.
Renger, G. (1976).Biochim. Biophys. Acta 440, 287–300.
Satoh, K. (1979).Biochim. Biophys. Acta 546 84–92.
Satoh, K. (1981). InProc. 5th Int. Photosynthesis Congress (Akoyunoglou, G., ed.), Vol.II pp. 607–616.
Satoh, K., and Butler, W. L. (1978).Plant Physiol. 61 373–379.
Satoh, K., and Mathis, P. (1981).Photobiochem. Photobiophys. 2, 189–198.
Steinback, K. E., Pfister, K., and Arntzen, C. J. (1981).Z. Naturforsch. Teil C 36 98–108.
Thomas, P. E., Ryan, D., and Levin, W. (1976).Anal. Biochem. 75 168–176.
Tischer, W., and Strotmann, H. (1979).Z. Naturforsch. Teil C 34 992–995.
Trebst, A., and Draber, W. (1979). InAdvances in Pesticide Science (Geissbühler, H., ed.), Part 2, Pergamon Press, Oxford New York, pp. 223–234.
Trebst, A. (1980).Methods Enzymol. 69 675–715.
Trebst, A., Reimer, S., Draber, W., and Knops, H. J. (1979).Z. Naturforsch. Teil C 34 831–840.
van Rensen, J. J. S., Wong, D., and Govindjee (1978).Z. Naturforsch. Teil C 33 413–420.
Velthuys, B. R., and Amesz, J. (1974).Biochim. Biophys. Acta 333 85–94.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Johanningmeier, U., Neumann, E. & Oettmeier, W. Interaction of a phenolic inhibitor with photosystem II particles. J Bioenerg Biomembr 15, 43–66 (1983). https://doi.org/10.1007/BF00743219
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00743219