Summary
Human term placenta and two human choriocarcinoma cell lines were studied immunohistochemically and by immunoblotting with monoclonal antibodies to keratin polypeptides and vimentin. Four keratin polypeptides (40, 45, 52, 54 K) were detected in both normal and malignant trophoblastic cells. The presence of the 54 K keratin polypeptide distinguishes the benign and malignant trophoblastic cells from human embryonal carcinoma cells and a yolk sac carcinoma cell line.
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Andrews PW, Damjanov I, Simon D, Banting GS, Carlin C, Dracopoli NC, Fogh J (1984) Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiationin vivo andin vitro. Lab Invest 50:147–162
Benham FJ, Wiles MV, Banting G, Andrews PW, Goodfellow PN (1983) Human-mouse teratocarcinoma hybrids: A tool for analysis of gene activity in early human development. In: Damjanov I, Knowles BB, Solter D (ed) The Human Teratomas. Experimental and Clinical Biology, Humana Press Clifton NJ, pp 313–341
Cooper D, Schermer A, Sun T-T (1985) Classification of epithelia and their neoplasms using monoclonal antibodies to keratins. Stratagies, applications and limitations. Lab Invest 52:243–256
Damjanov I (1984) Recent advances in the understanding of the pathology of testicular germcell tumors. World J Urol 2:12–17
Damjanov I, Clark RK, Andrews PW (1984) Cytoskeleton of human embryonal carcinoma cells. Cell Different 15:133–139
Franke WW, Mayer D, Schmidt E, Denk H, Borenfreund E (1981) Differences of expression of cytoskeletal proteins in cultured rat hepatocytes and hepatoma cells. Exp Cell Res 131:345–365
Hochstetter AR von, Hedinger CE (1982) The differential diagnosis of germ cell tumors in theory and practice. A critical analysis of two major systems of classification and review of 389 cases. Virchows Arch [Pathol Anat] 396:247–277
Moll R, Franke W, Schiller D (1982) The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24
Moll R, Krepler R, Franke WW (1983) Complex cytokeratin polypeptide patterns observed in certain human carcinomas. Differentiation 23:256–269
Nelson WG, Battifora H, Santana H, Sun T-T (1984) Specific keratins as molecular markers for neoplasms with a stratified epithelial origin. Cancer Res 44:1600–1603
Osborn M, Weber K (1983) Biology of disease: tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest 48:372–394
Pattillo RA, Gey GO (1968) The establishment of a cell line of human hormone-synthesizing trophoblastic cellsin vitro. Cancer Res 28:1231–1236
Pattillo RA, Ruchert A, Hussa R, Bernstein R, Des E 1971) The Jar cell line-continuous human multihormone production and control. In Vitro 6:398–399
Ramaekers RCS, Huysman A, Moesker O, Kant A, Jap P, Herman C, Vooijs P (1983) Monoclonal antibody to keratin filaments specific for glandular epithelia and their tumors: use in surgical pathology. Lab Invest 49:353–361
Sun T-T, Eichner R, Nelson WG, Tseng SCG, Weiss RA, Jarvinen M, Woodcock-Mitchell J (1983) Keratin classes: molecular markers for different types of epithelial differentiation. J Invest Dermatol 81:109S-115S
Sun T-T, Eichner R, Schermer A, Cooper D, Nelson WG, Weiss RA (1984) Classification, expression, and possible mechanisms of evolution of mammalian epithelial keratins: a unifying model. In: Cancer Cells 1/The Transformed Phenotype. Cold Spring Harbor Laboratory, Cold Spring Harbor NY, pp 169–176
Suurmeijer AJH, DeBruijn HWA, Oosterhuis JW, Sleyfer DT, Schraffordt Koops H, Fleuren GJ (1982) Non-seminomatous germ cell tumors of the testis: immunohistochemical localization and serum levels of human chorionic gonadotropin (HCG) and pregnancy-specific beta-1-glycoprotein (SP-1); value of SP-1 as a tumor marker. Oncodev Biol Med 3:409–422
Towbin H, Staehlin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Tseng SCG, Jarvinen MJ, Nelson WG, Huang J-W, Woodcock-Mitchell J, Sun T-T (1982) Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody study. Cell 30:361–372
Vogelzang NJ, Bronson DL, Savino D, Fraley EE (1983) Anin vivo andin vitro model of human embryonal and yolk sac carcinoma. In: Silver LM, Martin GR, Strickland S (eds) Teratocarcinoma Stem Cells. Cold Spring Harbor Conferences on Cell Proliferation vol 10, Cold Spring Harbor, New York, pp 607–614
Woodcock-Mitchell J, Eichner R, Nelson WG, Sun T-T (1982) Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 95:580–588
Wu WJ, Parker LM, Binder NE, Beckett MA, Sinard JH, Griffiths CT, Rheinwald JG (1982) The mesothelial keratins: a new family of cytoskeletal proteins identified in cultured mesothelial cells and non-keratinizing epithelia. Cell 31:693–703
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Clark, R.K., Damjanov, I. Intermediate filaments of human trophoblast and choriocarcinoma cell lines. Vichows Archiv A Pathol Anat 407, 203–208 (1985). https://doi.org/10.1007/BF00737077
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DOI: https://doi.org/10.1007/BF00737077