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Penetration rates of osmium tetroxide with different fixation vehicles

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Zeitschrift für Zellforschung und mikroskopische Anatomie Abt. Histochemie Aims and scope Submit manuscript

Summary

The presence of any substance other than OsO4 in osmium tetroxide fixation mixtures causes a decrease in the penetration rate of the fixative. Fixation times should therefore be prolonged whenever a fixation mixture containing osmotically active substances is used.

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Literature

  • Bahr, G. F.: Osmium tetroxide and ruthenium tetroxide and their reactions with biologically important substances. Exp. Cell Res.7, 457–479 (1954).

    Google Scholar 

  • Bahr, G. F., G. Bloom andU. Friberg: Volume changes of tissues in physiological fluids during fixation in osmium tetroxide or formaldehyde and during subsequent treatment. Exp. Cell Res.12, 342–355 (1957).

    Google Scholar 

  • Bahr, G. F., Bloom, G. andE. Johannisson: Further studies on fixation with osmium tetroxide. Histochemie1, 113–118 (1958).

    Google Scholar 

  • Caulfield, J. G.: Effects of varying the vehicle for OsO4 in tissue fixation. J. Biophys. Biochem. Cytol.3, 827–830 (1957).

    Google Scholar 

  • Lorenzo, A. J. de: Electron microscopic observations on the taste buds of the rabbit. J. Biophys. Biochem. Cytol.4, 143–150 (1958).

    Google Scholar 

  • Palade, G. E.: A study of fixation for electron microscopy. J. exp. Med.95, 285–297 (1952).

    Google Scholar 

  • Rhodin, J.: Correlation of ultrastructural organisation and function in normal and experimentally changed proximal convoluted tubule cells of the mouse kidney. Diss. Karol. Inst. Stockholm 1954. 76 p.

  • Robertis, E. de, H. M. Gerschenfeld andF. Wald: Cellular mechanism of myelination in the central nervous system. J. Biophys. Biochem. Cytol.4, 651–656 (1958).

    Google Scholar 

  • Trujillo-Cenoz, O.: Electron microscope study of the rabbit gustatory bud. Z. Zellforsch.46, 272–280 (1957).

    Google Scholar 

  • Zetterquist, H. A.: The ultrastructural organisation of the columnar absorbing cells of mouse. An electron microskopic study including some experiments regarding the problem of fixation and an investigation of vitamin A deficiency. Diss. Karol. Inst. Stockholm 1956. 83 p.

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Authors and Affiliations

  1. Sabbatsberg Hospital, Karolinska Institutet, Institute of Pathology, Stockholm, Sweden

    L. Hagström & G. F. Bahr

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  1. L. Hagström
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  2. G. F. Bahr
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Hagström, L., Bahr, G.F. Penetration rates of osmium tetroxide with different fixation vehicles. Histochemie 2, 1–4 (1960). https://doi.org/10.1007/BF00736486

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  • DOI: https://doi.org/10.1007/BF00736486

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