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Synthesis of an endogeneous lectin, galectin-1, by human endothelial cells is up-regulated by endothelial cell activation

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Abstract

The pattern of expression of an endogenous lectin, galectin-1, was examined in human lymphoid tissue. Galectin-1 was detected in the endothelial cells lining specialized vessels, termed high endothelial venules, in activated lymphoid tissue, but not in a resting lymph node. Cultured endothelial cells (human aortic and umbilical vein endothelial cells (HAECs and HUVECs)) expressed galectin-1. Activation of the cultured endothelial cells increased the level of galectin-1 expression, as determined by ELISA, Northern blot analysis and high throughput cDNA sequencing. These results suggest that galectin-1 expressed by endothelial cells may bind to and affect the trafficking of cells emigrating from blood into tissues.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, UCLA School of Medicine, 10833 LeConte Ave, 90024-1732, Los Angeles, CA, USA

    Linda G. Baum, Mabel Pang, Doré Beynon & Judith A. Berliner

  2. Incyte Pharmaceuticals, 3330 Hillview Ave, 94304, Palo Alto, CA, USA

    Jeffrey J. Seilhamer & Wendy B. Levine

Authors
  1. Linda G. Baum
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  2. Jeffrey J. Seilhamer
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  3. Mabel Pang
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  4. Wendy B. Levine
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  5. Doré Beynon
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  6. Judith A. Berliner
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Baum, L.G., Seilhamer, J.J., Pang, M. et al. Synthesis of an endogeneous lectin, galectin-1, by human endothelial cells is up-regulated by endothelial cell activation. Glycoconjugate J 12, 63–68 (1995). https://doi.org/10.1007/BF00731870

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  • DOI: https://doi.org/10.1007/BF00731870

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