Summary
A bronchial carcinoid with globular intracytoplasmic inclusions is reported. The inclusions stain brown with Grimelius silver impregnation and some show distinct immunoreactivity for chromogranin A. Tumour cells stain positively with antisera to neuron specific enolase, chromogranin A and not with antisera against ACTH, somatostatin or S-100 protein. The cells show distinct immunoreactivity for cytokeratins and vimentin, which is particularly intense in the intracytoplasmic inclusions. Desmin and glial fibrillary acidic protein are absent. Ultrastructural analysis reveals that the inclusions are composed of aggregates of filaments of 8–10 nm of diameter, intrapping a few neurosecretory granules. Immunohistochemical and ultrastructural data support the hypothesis that the inclusions are composed of intermediate filaments, whose metabolism and synthesis have somehow been deranged.
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References
Alvarez FE (1980) Intracytoplasmic fibrillary inclusions in bronchial carcinoid. Cancer 46:144–151
An T (1978) Cytoplasmic inclusions in bronchial carcinoid. Hum Pathol 9:241–242
Azumi N, Battifora H (1987) The distribution of vimentin and keratin in epithelial and nonepithelial neoplasms. A comprehensive immunohistochemical study on formalin- and alcoholfixed tumours. Am J Clin Pathol 88:286–296
Barbareschi M, Frigo B, Ferrero S, Mariscotti C, Mosca L (1988) Bronchial carcinoid with S-100 positive sustentacular cells. Tumori 6:699–705
Barbareschi M, Mauri MF, Muscarà M, Mauri FA, Lo Re V (1987) S-100 protein in human lung neuroendocrine neoplasms. Histol Histopath 2:185–192
Blobel GA, Gould VE, Moll R, Lee I, Huzar M, Geiger B, Franke WW (1985) Coexpression of neuroendocrine markers and epithelial cytoskeletal proteins in bronchopulmonary neuroendocrine neoplasms. Lab Invest 52:39–51
Berger G, Berger F, Bejui F, Bouvier R, Rochet M, Feroldi J (1984) Bronchial carcinoid with fibrillary inclusions related to cytocheratins: an immunohistochemical and ultrastructural study with subsequent investigation of 12 foregut APU Domas. Histopathology 8:245–252
Carstens PHB, Broghamer WL (1978) Duodenal carcinoid with cytoplasmic whorls of microfilaments. J Pathol 124:235–238
Christen B, Trojanowski JQ, Pietra GG (1987) Immunohistochemical demonstration of phosphorylated and nonphosphorylated forms of human neurofilament subunits in human pulmonary carcinoids. Hum Pathol 18:997–1001
El-Saltry M, Lundqvist M, Wilander E (1986) Bronchial carcinoids and phaeochromocytomas. A comparative study with special emphasis on S-100 protein, serotonin and neuron specific enolase (NSE) immunoreactivity. Acta Pathol Microbiol Scand [A] 94:229–235
Fetissof F, Boivin F, Jobard P, Arbeille-Brassard B, Romet JL, Maillet MY (1982) Microfilamentous carcinoid of the thymus: correlation of ultrastructural study with Grimelius stain. Ultrastruct Pathol 3:9–15
Goldman JE, Horoupian DS, Jhonson AB (1980) Granulofilamentous inclusions in a meningioma. Cancer 46:156–161
Gould VE, Linnoila RI, Memoli VA, Warren WH (1983) Neuroendocrine components of the bronchopulmonary tract: hyperplasias, dysplasias and neoplasms. Lab Invest 49:519–537
Gown AM (1988) personal communication
Hoefler H, Auboeck L (1984) S-100 protein in carcinoiden. Verh Dtsch Ges Pathol 68:86–91
Hoefler H, Denk H, Lackinger E, Helleis G, Polak JM, Heitz PU (1986) Immunocytochemical demonstration of intermediate filament cytoskeleton proteins in human endocrine tissues and (neuro-)endocrine tumours. Virchows Arch [A] 409:609–626
Horwath E, Kovacs K (1978) Morphogenesis and significance of fibrous bodies in human pituitary adenomas. Virchows Arch [B] 27:69–78
Ironside JW, Royds JA, Taylor CB, Timperley WR (1985) Paraganglioma of the cauda equina: a histological, ultrastructural and immunocytochemical study of two cases with a review of the literature. J Pathol 145:195–201
Jasani B, Edwards RE, Thomas ND, Gibbs AR (1985) The use of vimentin antibodies in the diagnosis of malignant mesothelioma. Virchows Arch [A] 406:441–448
Leader M, Collins M, Patel J, Henry K (1987) Vimentin: an evaluation of its role as a tumour marker. Histopathology 11:63–72
Leff EL, Brooks JSJ, Trojanoski JA (1985) Expression of neurofilament and neuron-specific enolase in small cell tumours of skin using immunohistochemistry. Cancer 56:625–631
Lehto VP, Stenman S, Miettinen M, Dahl D, Virtanen I (1983) Expression of a neural type of intermediate filament as a distinguishing feature between oat cell carcinoma and other lung cancers. Am J Pathol 110:113–118
Lehto VP, Miettinen M, Virtanen I (1985) A dual expression of cytokeratin and neurofilaments in bronchial carcinoid cells. Int J Cancer 35:421–425
Mosca L, Ceresoli A, Anzanello E, Mauri F, Mariscotti C, Timossi R, Barbareschi M, Mezzetti M (1986) Neuroendocrine structures in normal and diseased human lung. Appl Pathol 4:147–161
Mosca L, Barbareschi M, Mauri MF, Munguia-Barrera J, Frigo B, Muscarà M, Valentini L, Mariscotti C (1988) Neuroendocrine lung structures and tumours: immunohistochemical study by specific markers. Histol Histopath 3:367–376
Neumann PE, Goldman JE, Horoupian DS, Hess MA (1985) Fibrous bodies in growth-hormone-secreting adenomas countain cytokeratin filaments. Arch Pathol Lab Med 109:505–508
Reznik-Schuller H (1977) Ultrastructural alterations of APUD cells during nitrossamine-induced lung carcinogenesis. J Pathol 121:79–82
Riddel K, Tippens D, Gown AM (1987) PHE 5, a new monoclonal antibody to a unique neuroendocrine granule protein. Lab Invest 56:64A
Roy S (1978) Cytoplasmic filamentous masses in chromophobe adenoma of the human pituitary gland. J Pathol 125:151–154
Sajjad SM, Mackay B (1982) Hyaline inclusions in a synovial sarcoma following intra-arterial chemotherapy. Ultrastruct Pathol 3:313–319
Schochet SS, Mc Cormich WF, Almi NS (1972) Acidophilic adenomas with intra-cytoplasmic filamentous aggregates. A light and electron microscopic study. Arch Pathol 94:16–22
Sternberger LA (1979) Immunocytochemistry, 2nd ed. New York, John Wiley and Sons
Upton MP, Hirohashi S, Tome Y, Miyazawa N, Suemsu K, Shimosato Y (1986) Expression of vimentin in surgically resected adenocarcinomas and large cells carcinomas of lung. Am J Surg Pathol 10:560
Warner TFCS, Seo IS (1980) Aggregates of cytofilaments as the cause of the appearance of hyaline tumor cells. Ultrastruct Pathol 1:395–401
Wick MR, Scheithauer BW (1984) Thymic carcinoid. A histologic, immunohistochemical and ultrastructural study of 12 cases. Cancer 53:475
Wilander E, Westermark P, Grimelius L (1980) Intracellular and extracellular fibrillar structures in gastroduodenal endocrine tumors. Ultrastruct Pathol 1:49–54
Whittaker MH, Sandusky GE (1988) Immunoreactivity in abdominal carcinoids. Mod Pathol 1:102A
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Barbareschi, M., Frigo, B., Cristina, S. et al. Bronchial carcinoid with paranuclear fibrillary inclusions related to cytokeratins and vimentin. Vichows Archiv A Pathol Anat 415, 31–37 (1989). https://doi.org/10.1007/BF00718602
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DOI: https://doi.org/10.1007/BF00718602