Abstract
Biovar 1 strains ofAgrobacterium tumefaciens have been used to transform a cell suspension culture ofVitis vinifera cv. Cabernet Sauvignon. Cocultivation of cultures withAgrobacterium strains bearing either the cointegrate pGV3850::1103neo, or the binary vector pGA474-68, each gave rise to kanamycin resistant tissue. The stable integration and expression of the neomycin phosphotransferase gene was confirmed by Southern blotting and enzymic assay, respectively.
Similar content being viewed by others
Abbreviations
- NPTII:
-
neomycin phosphotransferase II
- NOS:
-
nopaline synthase
- FSAC:
-
fragmented shoot apex culture
- TES:
-
N-tris-(hydroxymethyl)methyl-2-aminoethane sulfonic acid
References
An, G. (1985) Plant Physiol. 79:568–570.
An, G., Watson, B.D., Stachel, S., Gordon, M.P., Nester, E.W. (1985) EMBOJ. 4:277–284.
Barlass, M., Skene, K.G.M. (1978) Vitis 17:335–340.
Bevan, M.W. (1984) Nucl. Acids Res. 12:8711–8721.
Bradford, M. (1976) Anal. Biochem. 72:248–254.
Burr, T.J., Katz, B.H., Bishop, A.L., Meyers, C.A., Mittak, V.L. (1988) Am. J. Enol. Vitic. 39:67–70.
Dandekar, A.M., Gupta, P.K., Durzan, D.J., Knauf, V. (1987) Biotechnology 5:587–590.
Ditta, G., Stanfield, S., Gorbin, D., Helinski, D.R. (1980) Proc. Nat. Acad. Sci. U.S.A. 77:7347–51.
Fillatti, J.J., Sellmer, J., McCown, B., Haissig, B., Comai, L. (1987) Mol. Gen. Genet. 26:192–199.
Hawker, J.S., Downton, W.J.S., Wiskich, D., Mullins, M.G. (1973) Hort Science 8:398–399.
Hawker, J.S., Downton, W.J.S. (1974) Phytochemistry 13:893–900.
Hemstad, P.R., Reisch, B.I. (1985) J. Plant Physiol. 120:9–17.
Hood, E.E., Hellmer, G.L., Fraley, R.T., Chilton, M.D. (1986) J. Bacteriol. 168:1291–1301.
Horsch, R.B., Fry, J.E., Hoffman, N.L., Eicholtz, D., Rogers, S.G., Fraley, R.T. (1985) Science 227:1229–31.
Knauf, V.C., Panagopoulos, C.G., Nester, E.W. (1982) Phytopathology 72:1545–49.
McGranahan, G.H. Leslie, C.A., Uratsu, S.L., Martin, L.A., Dandekaar, A.M. (1988) Biotechnology 6:800–804.
Parsons, T.J., Sinkar, V.P., Stettler, R.F., Nester, E.W., Gordon, M.P. (1986) Biotechnology 4:533–536.
Pythoud, F., Sinkar, V.P., Nester, E.W., Gordon, M.P. (1987) Biotechnology 5:1323–27.
Reiss, B., Sprengel, R., Will, H., Schaller, H. (1984) Gene 30:211–218.
Scott, N.S., Possingham, J.V. (1980) J. Exp. Bot. 31:1081–92.
Scott, N.S., Timmis, J.N. (1984) Theor. Appl. Genet. 67:279–288.
Sederoff, R., Stomp, A.M., Chilton W.S., Moore, L.W. (1986) Biotechnology 4:647–649.
Stachel, S.E., Messens, E., Van Montagu, M., Zambryski, P. (1985) Nature 318:624–29.
Yanofsky, M., Lowe, B., Montoya, A., Rubin, R., Krul, W., Gordon, M., Nester, E. (1985) Mol. Gen. Genet. 201:237–246.
Zorkowski, W., Lorkiewicz, Z. (1978) Genet. Res. 32:311–314.
Author information
Authors and Affiliations
Additional information
Communicated by R. Beachy
Rights and permissions
About this article
Cite this article
Baribault, T.J., Skene, K.G.M. & Steele Scott, N. Genetic transformation of grapevine cells. Plant Cell Reports 8, 137–140 (1989). https://doi.org/10.1007/BF00716825
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00716825