Abstract
In order to investigate the cellular basis of salt tolerance, Colt cherry (Prunus avium ×pseudocerasus) protoplasts from mesophyll tissues and root cell suspension cultures were cultured in the presence of NaCl, KCl or Na2SO4, at normalities of 25, 50, 100 or 200 mN for each salt and with or without 2,6-dichlorobenzonitrile, an inhibitor of cell wall synthesis. Results showed that the acquisition of salt tolerance was concomitant with the onset of cell wall regeneration, with protoplasts exhibiting a greater salt tolerance than cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- DBN:
-
2,6-dichlorobenzonitrile
- FDA:
-
fluorescein diacetate
References
Ben-Hayyim G (1987) Plant Physiol 85: 430–433
Galbraith DW, Shields BA (1982) Physiol Plant 55: 25–30
Hasegawa PM, Bressan RA, Handa AK (1986) Hort Sci 21: 1317–1324
Kochba J, Ben-Hayyim G, Spiegel-Roy P, Saad S, Neumann H (1982) Z Pflanzenphysiol 106: 111–118
Ochatt SJ, Power JB (1989) Tree Physiol (in press)
Ochatt SJ, Cocking EC, Power JB (1987) Plant Sci 50: 139–143
Power JB, Chapman JV, Wilson D (1984) Laboratory Manual, Plant Tissue Culture, Plant Genetic Manipulation Group, University of Nottingham, UK
Rech EL, Ochatt SJ, Chand PK, Mulligan BJ, Davey MR, Power JB (1988) Bio/Technology 6: 1091–1093
Rosen A, Tal M (1981) Z Pflanzenphysiol 102: 91–94
Scheffe H (1959) The analysis of variance. John Wiley & Sons Inc, Canada
Author information
Authors and Affiliations
Additional information
Communicated by N. Amrhein
Rights and permissions
About this article
Cite this article
Ochatt, S.J., Power, J.B. Cell wall synthesis and salt (saline) sensitivity in cultured colt cherry (Prunus avium × pseudocerasus) protoplasts. Plant Cell Reports 8, 365–367 (1989). https://doi.org/10.1007/BF00716675
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00716675