Abstract
Explants fromPisum sativum shoot cultures and epicotyls were transformed by cocultivation withAgrobacterium tumefaciens vectors carrying plant selectable markers and transformants could be selected on a medium containing kanamycin. Transformants could also be obtained at a low frequency by cocultivating small protoplast-derived colonies. The transformed nature of the calli obtained from selection was confirmed by opine assay and DNA analysis. In addition five cultivars of pea were tested for their response to seven differentAgrobacterium tumefaciens strains. The response pattern coincided largely between the different pea cultivars, being more dependent on the bacterial strain than the cultivar used.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BA:
-
6-benzyladenine
- Km:
-
kanamycin
- NAA:
-
∝-naphthaleneacetic acid
- NOS:
-
nopaline synthase
- NPT:
-
neomycin phosphotransferase
- OCS:
-
octopine synthase
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Puonti-Kaerlas, J., Stabel, P. & Eriksson, T. Transformation of pea (Pisum sativum L.) byAgrobacterium tumefaciens . Plant Cell Reports 8, 321–324 (1989). https://doi.org/10.1007/BF00716664
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DOI: https://doi.org/10.1007/BF00716664