Abstract
Human glioma cells (138MG) have a low-affinity uptake system for choline (Km = 20 µM; Vmax = 56 pmol/min/106 cells). The uptake is reduced by acetylcholine, hemicholinium-3, HgCl2, and phosphodiesterase inhibitors. Release of [3H]choline from preloaded cultures showed two pools with half-lives of 1.3 and 160 min. Choline release was stimulated by 8-bromo-cAMP or isobutylmethylxanthine. The results suggest that release of choline occurs by a facilitated diffusion transport system and is increased by elevations of intracellular cAMP.
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Walum, E. Uptake and release of choline in cultures of human glioma cells. Cell Mol Neurobiol 1, 389–399 (1981). https://doi.org/10.1007/BF00716273
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DOI: https://doi.org/10.1007/BF00716273