Summary
Renal biopsy specimens from 11 children with Henoch-Schönlein nephritis and 14 with IgA nephropathy were examined by immunoelectron microscopy. The distribution of IgA reaction product (RP) was found to be similar to that of the electron-dense deposits seen with conventional electron microscopy. Deposits of IgA-RP were present in the mesangium and in the subendothelial region of the peripheral glomerular capillary wall in all patients. Subepithelial deposits of IgA-RP were seen in 12 patients. Deposits of IgG-RP were rare and no deposits of IgM-RP were observed. Deposits of C3-RP were found frequently, although they were smaller and less extensive than deposits of IgA-RP. There was no significant difference between Henoch-Schönlein nephritis and IgA nephropathy with regard to immunoelectron microscopy findings. These observations suggest that Henoch-Schönlein nephritis and IgA nephropathy are both forms of mesangiopathic glomerulonephritis caused by immune complexes, mainly composed of IgA.
Similar content being viewed by others
References
Couser WG, Salant DJ (1980) In situ immune complex formation and glomerular injury. Kidney Int 17:1–13
Davies DR, Tighe JR, Wing AJ, Jones NF (1977) Immunoglobulin deposition in membranous glomerulonephritis: immunofluorescence and immuno-electron microscopy findings. Histopathology 1:39–52
Davies M, Barret AJ, Travis J, Sanders E, Coles GA (1978) The degeneration of human glomerular basement membrane with purified lysosomal proteinases: evidence for the pathogenic role of the polymorphonuclear leukocyte in glomerulonephritis. Clin Sci Mol Med 54:233–240
Germuth FG, Rodriguez E (1975) Focal mesangiopathic glomerulonephritis: prevalence and pathogenesis. Kidney Int 7:216–223
Kondo Y, Shigematsu H, Kobayashi Y (1972) Cellular aspects of rabbit Masugi nephritis II: progressive glomerular injuries with crescent formation. Lab Invest 27:620–631
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative: A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Michael AF, Keane WF, Raij L, Vernier RL, Mauer SM (1980) The glomerular mesangium. Kidney Int 17:141–154
Morita T, Suzuki Y, Churg J (1973) Structure and development of the glomerular crescent. Am J Pathol 72:349–368
Naish PF, Thomson NM, Simpson IJ, Peters DK (1975) The role of polymorphonuclear leukocytes in the autologous phase of nephrotoxic nephritis. Clin Exp Immunol 22:102–109
Sindrey M, Naish P (1979) The mediation of polymorphonuclear leukocytes in glomeruli during the autologous phase of nephrotoxic nephritis. Clin Exp Immunol 35:350–355
Wilson MB, Nakane PK (1978) Recent development in the periodate method of conjugating horseradish peroxidase to antibodies. In: Knapp W, Holubar K, Wick G (eds) Immunofluorescence and related staining techniques. Elsevier, Amsterdam, pp 215–224
Yoshikawa N, Uehara S, Yamana K, Ikeuchi H, Hiraumi M, Matsuo T, Okada S (1980) Clinicopathological study of persistent asymptomatic proteinuria in children. Nephron 25:127–133
Yoshikawa N, White RHR, Cameron AH (1981) Prognostic significance of the glomerular changes in Henoch-Schönlein nephritis. Clin Nephrol 16:223–229
Yoshikawa N, Ito H, Nakahara C, Yoshiara S, Yoshiya K, Matsuo T, Hasegawa O, Hazikano H, Okada S (1985) Glomerular electron-dense deposits in childhood IgA nephropathy. Virchows Arch A 406:33–43
Yoshikawa N, Yoshiara S, Yoshiya K, Matsuo T, Okada S (1986) Lysis of the glomerular basement membrane in children with IgA nephropathy and Henoch-Schönlein nephritis. J Pathol 150:119–126
Yoshikawa N, Ito H, Yoshiara S, Nakahara C, Yoshiya K, Hasegawa O, Matsuo T (1987a) Clinical course of IgA nephropathy in children. J Pediatr 110:555–560
Yoshikawa N, Ito H, Yoshiya K, Nakahara C, Yoshiara S, Hasegawa O, Matsuyama S, Matsuo T (1987b) Henoch-Schönlein nephritis and IgA nephropathy in children: A comparison of clinical course. Clin Nephrol (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yoshiara, S., Yoshikawa, N. & Matsuo, T. Immunoelectron microscopic study of childhood IgA nephropathy and Henoch-Schönlein nephritis. Vichows Archiv A Pathol Anat 412, 95–102 (1987). https://doi.org/10.1007/BF00716180
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00716180