Summary
The present study concerns the immunocytochemical localization of S-100 protein α and β subunits in the cells of human lymphoreticular tissue and their related tumours. The α subunit is mainly localized in dendritic cells, most likely the dendritic reticulum cells (DRCs) located within the germinal centers, while the β subunit is mainly localized in the interdigitating reticulum cells (IRCs) in the paracortical area and in Histiocytosis “X” cells. No immunoreactivity for either subunit was found in the majority of normal lymphocytes, macrophages, malignant lymphoma cells, or xanthoma cells.
The DRCs and IRCs are generally considered to show different distribution in the lymphoid tissues and demonstrate some difference in their immunocytochemical and enzyme-histochemical features. It is suggested that S-100 subunits can be used as useful markers for these two types of dendritic cells and investigation of these subunits may provide more information for the study of human lymphoreticular system.
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References
Beckstead JH (1983) The evaluation of human lymph nodes, using plastic sections and enzyme histochemistry. Am J Clin Pathol 80:131–139
Carbone A, Manconi R, Poletti A, Volpe R, Santi L (1985) S-100 protein immunostaining in cells of dendritic morphology within reactive germinal centers by ABC immunoperoxidase method. Virchows Arch [Pathol Anat] 406:27–32
Carbone A, Poletti A, Manconi R, Volpe R, Santi L (1985) Demonstration of S-100 protein distribution in human lymphoid tissues by the Avidin-Biotin Complex immunostaining method. Hum Pathol 16:1157–1164
Cocchia D, Michetti F, Donato R (1981) Immunochemical and immunocytochemical localization of S-100 antigen in normal human skin. Nature 294:85–87
Ide F, Iwase T, Saito I, Umemura S, Nakajima T (1984) Immunohistochemical and ultrastructural analysis of the proliferating cells in histiocytosis X. Cancer 53:917–921
Isobe T, Okuyama T (1978) The amino acid sequence of S-100 protein (PAP-Ib protein) and its relation to the calcium-binding proteins. Eur J Biochem 89:379–388
Isobe T, Okuyama T (1981) The amino acid sequence of the subunit in bovine brain S-100ao protein. Eur J Biochem 166:79–86
Kahn HJ, Marks LA, Thom H, Baumal R (1983) Role of antibody to S-100 protein in diagnostic pathology. Am J Clin Pathol 79:341–347
Klaus GGB, Humphrey JH, Kunkl A, Dongworth DW (1980) The follicular dendritic cell: Its role in antigen presentation in the generation of immunological memory. In: Möller G (ed), Immunological Rev, vol 53, Munksgaard, Copenhagen, pp 3–28
Manabe T, Takahashi Y, Okuyama T (1983) Analysis of human haptoglobin-hemoglobin complexes by micro two-dimensional electrophoresis. Electrophoresis 4:359–362
Manabe T, Takahashi Y, Okuyama T (1984) An electroblotting apparatus for multiple replica technique and identification of human serum proteins on micro two-dimensional gels. Anal Biochem 143:39–45
Moore BW (1965) A soluble protein characteristic of the nervous system. Biochem Biophys Res Commun 19:739–744
Moore BW (1972) Chemistry and biology of two proteins, S-100 and 14-3-2 specific to the nervous system. Int Rev Neurobiol 15:215–225
Müller-Hermelink HK, Lennert K (1978) The cytologic, histologic and functional bases for a modern classification of lymphomas. In: Lubarsch O, Henke F, Rossle R, Uehlinger E (eds) Handbuch der Speziellen Pathologischen Anatomie und Histologie Bandteil B. Malignant Lymphomas other than Hodgkin's disease. Springer, Berlin Heidelberg New York, pp 59–68
Murphy GF, Bhau AK, Sato S, Mihm MC, Harrist TJ (1981) A new immunologic marker for human Langerhans cells. New Engl J Med 304:781–792
Naiem M, Gerdes J, Abdulaziz Z, Stein H, Mason DY (1983) Production of a monoclonal antibody reactive with human dendritic reticulum cells and its use in the immunohistochemical analysis of lymphoid tissues. J Clin Pathol 36:167–175
Nakajima T, Watanabe S, Sato Y, Kameya T, Hirota T, Shimosato Y (1982) An immunoperoxidase study of S-100 protein distribution in normal and neoplastic tissues. Am J Surg Pathol 6:715–727
Nakajima T, Watanabe S, Sato Y, Shimosato Y, Motoi M, Lennert K (1982) S-100 protein in Langerhans' cells, interdigitating reticulum cells, and histiocytosis X cells. Gann 73:429–432
Rausch ER, Kaiserling E, Goos M (1977) Langerhans cells and interdigitating reticulum cells in the thymus-dependent region in human dermatopathic lymphadenitis. Virchows Arch [Cell Pathol] 25:327–343
Silberberg-Sinakin I, Gigli I, Baer RL, Thorbecke GJ (1980) Langerhans cells: Role in contact hypersensitivity and relationship to lymphoid dendritic cells and to macrophages. In: Miller G (ed), Immunological Rev. vol 53, Munksgaard, Copenhagen, pp 203–232
Takahashi K, Yamaguchi H, Ishizeki J, Nakajima T, Nakazato Y (1981) Immunohistochemical and immunoelectron microscopic localization of S-100 protein in the interdigitating reticulum cells of the human lymph node. Virchows Arch [Cell Pathol] 37:125–135
Takahashi K, Isobe T, Ohtsuki Y (1984) Immunohistochemical localization and distribution of S-100 protein in the human lymphoreticular system. Am J Pathol 116:497–50
Takahashi K, Isobe T, Ohtsuki Y, Sonobe H, Yamaguchi H, Akagi T (1985) S-100 protein positive human T-lymphocytes. Am J Clin Pathol 83:69–72
Tanaka Y, Misugi K, Okuda K (1986) An immunocytochemical study on subunits of S-100 protein distribution in normal tissues. IGAKU NO AYUMI 136:303–304 (in Japanese)
Valk P van der, Loo EM van der, Jansen J, Daha MR, Meijer CJLM (1984) Analysis of lymphoid and dendritic cells in human lymph node, tonsile and spleen. Virchows Arch [Pathol Anat] 45:169–185
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Tanaka, Y. Immunocytochemical study of human lymphoid tissues with monoclonal antibodies against S-100 protein subunits. Vichows Archiv A Pathol Anat 410, 125–132 (1987). https://doi.org/10.1007/BF00713516
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DOI: https://doi.org/10.1007/BF00713516