Summary
Sulphite oxidase deficiency occurs in man in two forms, as the isolated deficiency and as a syndrome of combined molybdoenzyme deficiency. This latter pleiotropic condition has as its underlying cause a defect in the synthesis of the molybdenum cofactor required for the activity of all molybdoenzymes in humans. Difficulties in diagnosis of sulphite oxidase deficiency are often encountered. A new method for detection of a key diagnostic metabolite,S-sulphocysteine, is outlined. The procedure is based on precolumn derivatization of urinary amino acids with dimethylaminoazobenzene sulphonyl chloride (Dabsyl-Cl) and resolution of the modifiedS-sulphocysteine by reversed-phase HPLC. A number of affected patients and control individuals with similar clinical symptoms have been studied, and a clear demarcation between the two groups has been noted.
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Johnson, J.L., Rajagopalan, K.V. An HPLC assay for detection of elevated urinaryS-sulphocysteine, a metabolic marker of sulphite oxidase deficiency. J Inherit Metab Dis 18, 40–47 (1995). https://doi.org/10.1007/BF00711371
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DOI: https://doi.org/10.1007/BF00711371