Skip to main content
SpringerLink
Log in

General characteristics of the polytene chromosomes from ovarian pseudonurse cells of theDrosophila melanogaster otu 11 andfs(2)B mutants

  • Published:
Chromosome Research Aims and scope Submit manuscript

Abstract

Polytene chromosomes of good cytological quality from pseudonurse cells (PNCs) offs(2)B andotu 11 mutants were obtained, photomaps forotu 11 mutants were constructed and the general characteristics of polytene chromosomes from salivary glands (SGs) and PNCs were compared. Three conditions were found to improve the cytological quality of PNC chromosomes: temperature below 18°C, a protein-rich medium and presence of the Y-chromosome. Detailed comparison of the chromosome banding pattern from SGs and PNCs has shown only minor differences between them. The frequency of asynapsis appeared to be 10 times higher for PNC chromosomes. Despite previous reports, features such as breaks and ectopic contacts turned out to be also typical for PNC chromosomes, but with remarkably lower frequencies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beermann W (1952) Chromomerenkonstanz and spezifische Modifikationen der Chromosomenstruktur in der Entwicklung und Organdifferenzierung vonChironomus tentans.Chromosoma 5: 139–198.

    Google Scholar 

  • Beermann W (1972) Chromomeres and genes. In: Beerman W, ed.Developmental Studies on Giant Chromosomes. Results and Problems in Cell Differentiation Vol. 4. Berlin: Springer-Verlag, pp. 12–13.

    Google Scholar 

  • Belyaeva ES (1973) Asynapsis of homologues inDrosophila melanogaster salivary chromosomes.DIS 50: 40.

    Google Scholar 

  • Coluzzi M, Cancrini G, Di Deco M (1970) The polytene chromosomes ofAnopheles superpictus and relationships withAnopheles stephensi.Parasitologia 12: 101–112.

    Google Scholar 

  • Dabbs CK, King RC (1980) The differentiation of pseudonurse cells in the ovaries offs231 females ofDrosophila melanogaster Meigen (Diptera: Drosophilidae).Int J Insect Morphol Embryol 9: 215–229.

    Google Scholar 

  • Ephrussi B, Beadle GW (1936) A technique of transplantation forDrosophila.Am Nat 70: 218–225.

    Google Scholar 

  • Freed JJ, Schultz J (1956) Effect of the Y-chromosome on the desoxyribonucleic acid content of ovarian nuclei inDrosophila melanogaster females.J Histochem Cytochem 4: 441–442.

    Google Scholar 

  • Gupta JP, Singh OP (1983) Puffing patterns in polytene chromosomes of different tissues ofMelanagromyza obtusa during development.J Hered 74: 365–370.

    Google Scholar 

  • Heino TI (1989) Polytene chromosomes from ovarian pseudonurse cells of theDrosophila melanogaster otu mutant. I. Photographic map of chromosome 3.Chromosoma 97: 363–373.

    Google Scholar 

  • Heino TI (1994) Polytene chromosomes from ovarian pseudonurse cells of theDrosophila melanogaster otu mutant. II. Photographic map of the X chromosome.Chromosoma 102: 4–15.

    Google Scholar 

  • Hochstrasser M (1987) Chromosome structure in four wild-type polytene tissues ofDrosophila melanogaster. The 87A and 87C heat shock loci are induced unequally in the midgut in a manner dependent on growth temperature.Chromosoma 95: 197–208.

    Google Scholar 

  • Holden JJ, Ashburner M (1978) Patterns of puffing activity in the salivary gland chromosomes ofDrosophila. IX. The salivary and protoracic gland chromosomes of a dominant temperature sensitive lethal ofD. melanogaster.Chromosoma 68: 205–227.

    Google Scholar 

  • Kastritsis CD, Grossfield J (1971) Balbiani rings inD. auraria.DIS 47: 123.

    Google Scholar 

  • King RC, Bahns M, Horowitz R, Larramendi P (1978) A mutation that affects female and male germ cells differentially inDrosophila melanogaster Meigen (Diptera: Drosophilidae).Int J Insect Morphol Embryol 7: 359–375.

    Google Scholar 

  • King RC, Riley SF, Cassidy JD, White P, Paik Y (1981) Giant polytene chromosomes from the ovaries of aDrosophila mutant.Science 212: 441–443.

    Google Scholar 

  • Lefevre G (1976) A photographic representation and interpretation of the polytene chromosomes ofDrosophila melanogaster salivary glands. In: Ashburner M, Novitski E, eds.The Genetics and Biology of Drosophila, Vol 1a. London: Academic Press, pp 31–66.

    Google Scholar 

  • Lindsley DL, Zimm GG (1992)The Genome of Drosophila melanogaster. San Diego: Academic Press, inc. Harcourt Brace Jovanovich.

    Google Scholar 

  • Mal'ceva NI, Zhimulev IF (1993) Extent of polyteny in the pericentric heterochromatin of polytene chromosomes of pseudonurse cells ofotu (ovarian tumor) mutants ofDrosophila melanogaster.Mol Gen Genet 240: 273–276.

    Google Scholar 

  • Redfern CPF (1981) Homologous banding patterns in the polytene chromosomes from the larval salivary glands and ovarian nurse cells ofAnopheles stephensi Liston (Culicidae).Chromosoma 83: 221–240.

    Google Scholar 

  • Ribbert D (1979) Chromomeres and puffing in experimentally induced polytene chromosomes ofCalliphora erythrocephala.Chromosoma 74: 269–298.

    Google Scholar 

  • Richards G (1980) The polytene chromosomes in the fat body nucleiDrosophila melanogaster.Chromosoma 79: 241–250.

    Google Scholar 

  • Robert PA (1988) Developmental changes in midgut chromosomes ofDrosophila gibberosa.Chromosoma 97: 254–260.

    Google Scholar 

  • Scouras ZG, Kastritsis CD (1984) Balbiani rings and puffs of the polytene chromosomes ofDrosophila auraria.Chromosoma 89: 96–106.

    Google Scholar 

  • Sinha P, Mishra A, Lakhotia SC (1987) Chromosomal organization ofDrosophila tumours. I. Polytene chromosome organization and DNA synthesis in ovarian pseudonurse cells inotu mutants ofD. melanogaster.Chromosoma 95: 108–116.

    Google Scholar 

  • Stalker HD (1954) Banded polytene chromosomes in the ovarian nurse cells of adult Diptera.J Hered 45: 259–264.

    Google Scholar 

  • Stegniy VN (1979) Reorganization of interphase nuclei structure of malaria mosquito in onto- and phylogenesis.Docl Acad Sci USSR 249: 1231–1234.

    Google Scholar 

  • Zhimulev IF (1992)Polytene Chromosomes: Morphology and Structure Novosibirsk: Nauka, pp 1–479.

    Google Scholar 

  • Zhimulev IF, Belyaeva ES Variation of banding pattern in polytene chromosomes of Drosophila melanogaster larvae (1977)Genetica (USSR) 13: 1398–1407.

    Google Scholar 

  • Zhimulev IF, Semeshin VF, Kulichkov VA, Belyaeva ES (1982) Intercalary heterochromatin inDrosophila I. Localization and general characteristics.Chromosoma 87: 197–228.

    Google Scholar 

  • Zhimulev IF, Pokholkova GV, Bgatov AV, Semeshin VF, Umbetova GH, Belyaeva ES (1983) Genetic interpretation of polytene chromosomes banding pattern.Mol Biol Rep 9: 19–23.

    Google Scholar 

  • Zhimulev IF, Belyaeva ES, Bolshakov VN, Mal'ceva NI (1989) Position-effect variegation and intercalary heterochromatin: A comparative study.Chromosoma 98: 378–387.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. the Laboratory of Molecular Cytogenetics, Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, 630090, Novosibirsk, Russian Federation

    N. I. Mal'ceva, H. Gyurkovics & I. F. Zhimulev

Authors
  1. N. I. Mal'ceva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Gyurkovics
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. F. Zhimulev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. I. Mal'ceva.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mal'ceva, N.I., Gyurkovics, H. & Zhimulev, I.F. General characteristics of the polytene chromosomes from ovarian pseudonurse cells of theDrosophila melanogaster otu 11 andfs(2)B mutants. Chromosome Res 3, 191–200 (1995). https://doi.org/10.1007/BF00710713

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00710713

Key words

Search

Navigation