Abstract
The 5′ to 3′ direction of DNA strands within chromatids of metaphase chromosomes can be determined by using simultaneous hybridization of a single strand of the telomere probe and a single strand of a repetitive sequence to slides pretreated for strand-specific hybridization. The telomere probe identifies the direction of the DNA helical strand remaining in each chromatid of the metaphase chromosomes. The direction of the repetitive sequence is then determined from the direction of the strand to which it hybridizes. This method was used to determine the 5′ to 3′ direction of three repetitive DNA sequences, each for a different human repeat family.
Similar content being viewed by others
References
Buroker N, Bestwick R, Haight G, Magenis RE, Litt M (1987) A hypervariable repeated sequence on human chromosome 1p36.Hum Genet 77: 175–181.
de Lange T, Shiue L, Myers RMet al. (1990) Structure and variability of human chromosome ends.Mol Cell Biol 10: 518–527.
Devilee P, Slagboom P, Cornelisse C, Pearson P (1986) Sequence heterogeneity within the human alphoid repetitive DNA family.Nucleic Acids Res 14: 20059–2073.
Goodwin E, Meyne J (1993) Strand-specific FISH reveals orientation of chromosome 18 alphoid DNA.Cytogenet Cell Genet 63: 126–127.
Henderson ER, Blackburn EH (1989) An overhanging 3′ terminus is a conserved feature of telomeres.Mol Cell Biol 9: 345–348.
Meyne J, Moyzis RK (1989) Human chromosome-specific repetitive DNA probes: targetingin situ hybridization to chromosome 17 with a 42 base pair alphoid DNA oligomer.Genomics 4: 472–478.
Meyne J, Goodwin EH, Moyzis RK (1994) Chromosomal localization and orientation of the simple sequence repeat of human satellite I DNA.Chromosoma 103: 99–103.
Moyzis RK, Albright KL, Bartholdi MFet al. (1987) Human chromosome specific repetitive DNA sequences: novel markers for genetic analysis.Chromosoma 95:375–386.
Moyzis RK, Buckingham JM, Cram LSet al. (1988)A highly conserved repetitive DNA sequence (TTAGGG) n ,present at the telomeres of human chromosomes.Proc Natl Acad Sci USA 85: 6622–6626.
Nakamura Y, Julier C, Wolff Ret al. (1987) Characterization of a human minisatellite sequence.Nucleic Acids Res 15: 2537–2547.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Meyne, J., Goodwin, E.H. Direction of DNA sequences within chromatids determined using strand-specific FISH. Chromosome Res 3, 375–378 (1995). https://doi.org/10.1007/BF00710019
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00710019