Summary
Dipeptidylpeptidase IV (DPP IV) occurs among others in exocrine epithelia, hepatocytes, renal tubuli, endothelia, and myofibroblasts of man and laboratory animals. Also Tµ lymphocytes and their varying differentiated neoplastic counterparts reveal this enzyme activity. The present paper describes a new monoclonal antibody recognizing DPP IV.
Additional efforts have been taken to detect the subcellular localization of DPP IV and its isoelectric focusing pattern in different tissue types. The monoclonal antibody anti-DPP IV (clone II-19) shows a reaction pattern indistinguishable from the corresponding enzymehistochemical reaction. These findings were further substantiated by immunoblotting analysis. In line with the results of direct enzyme measurements in different subcellular fractions a considerable portion of the enzyme is localized in the membrane fraction.
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Dedicated to Professor Dr.Dr. h.c. Karl Lennert, Kiel, on the occasion of his 65th birthday
This study was supported by the Deutsche Forschungsgemeinschaft, SFB 111, program CL1
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Feller, A.C., Radzun, HJ., Heymann, E. et al. A monoclonal antibody detecting dipeptidylpeptidase IV in human tissue. Vichows Archiv A Pathol Anat 409, 263–273 (1986). https://doi.org/10.1007/BF00708333
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DOI: https://doi.org/10.1007/BF00708333