Summary
Morphometric techniques for quantitating cytologic and volumetric changes in small intestinal mucosae are described: measurements were controlled with respect to a constant ‘test area’ of muscularis mucosae, thus allowing valid comparisons between normal and damaged mucosae. Procedures for enumerating cell populations within mucosal volume compartments are illustrated by analyzing lymphocytes within surface epithelium. These techniques serve to distinguish (i) absolute cell counts from (ii) relative cell counts (“densities”) the latter being shown to be considerably affected by changes in epithelial volume. They also permit construction of mathematical models e.g. cubes of equivalent volume, volume-density graphs, proportional volumes, which are illustrated.
Use of these morphometric procedures showed that there is no major difference in epithelial lymphocyte populations between untreated coeliac disease, and control, mucosae. The data thus fail to support the widespread view that “infiltration” of coeliac disease epithelium by lymphocytes represents a local cell-mediated immune reaction to gluten.
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References
Aherne WA, Dunnill MS (1982) Morphometry. Arnold, London
Chalkley HW, Cornfield J, Park H (1949) A method for estimating volume-surface ratios. Science 110:295–297
Chapman BL, Henry K, Paice F, Stewart JS, Coghill NF (1973) A new technique for examining intestinal biopsies. Gut 14:905–909
Chaudhuri RN, Saha TK (1963) Jejunal mucosa in malabsorption syndrome. J Ind Med Assoc 41:427–432.
Elias H, Hennig A, Schwartz DE (1971) Stereology: applications to biomedical research. Phys Rev 51:158–200
Ferguson A, Murray D (1971) Quantitation of intraepithelial lymphocytes in human jejunum. Gut 12:988–994
Gangarosa E, Beisel W, Benyajati C, Sprinz H, Piyarat NP (1960) The nature of the gastrointestinal lesion in Asiatic cholera and its relation to pathogenesis: a biopsy study. Am J Trop Med Hyg 9:125–135
Giger H, Riedwyl H (1970) Bestimmung der Größenverteilung von Kugeln aus Schnittkreisradien. Biometr Zeitschr 12:156–165
Guix M, Skinner JM, Whitehead R (1979) Measuring intra-epithelial lymphocytes, surface area, and volume of lamina propria on the jejunal mucosal of coeliac patients. Gut 20:275–278
Hammar FA (1926) Ueber Methoden, die Groesse und Anzahl der Hassalschen Koerper zahlenmaessig festzustellen. Z Mikroscop Anat Forsch 6:399–408
Holmes GK, Asquith P, Stokes PL, Cooke WT (1974) Cellular infiltration of jejunal biopsies in adult coeliac disease in relation to gluten withdrawal. Gut 15:278–283
Marsh MN, Brown AC, Swift JA (1970) The surface ultrastructure of the small intestinal mucosa of normal human control subjects and of patients with untreated and treated coeliac disease, using the scanning electron microscope. In: CC Booth, RH Dowling (eds) Coeliac disease. Churchill-Livingstone, Edinburgh, pp 26–43
Marsh MN (1972) The scanning electron microscope and its application to the investigation of intestinal structure. In: J Badenoch, BN Brooke (eds) Recent advances in gastroenterology. Churchill-Livingstone, Edinburgh, pp 81–135
Marsh MN (1980) Studies of intestinal lymphoid tissue. III-Quantitative analyses of epithelial lymphocytes in the small intestine of human control subjects and of patients with celiac sprue. Gastroenterology 79:481–492
Marsh MN, Mathan M, Mathan VI (1983) Studies of intestinal lymphoid tissue. VII - The secondary nature of lymphoid cell “activation” in the jejunal lesion of tropical sprue. Am J Pathol 112:302–312
Marsh MN (1984) The morphologic expression of immunologically-mediated change and injury within the human small intestine mucosa. In: R Batt, TLJ Lawrence (eds) Function and dysfunction of the small intestine in animals. Liverpool University Press, Liverpool pp 167–198
Miles RE, Davey PT (1977) On the choice of quadrats in stereology. J Microsc 10:27–44
Montgomery RD, Shearer ACI (1974) The cell population of the upper jejunal mucosa in tropical sprue and post-infective malabsorption. Gut 15:387–391
Otto HF (1972) Interepitheliale Lymphozyten bei Enteropathien. Z Gastroenterol 10:173–179
Padykula HA, Strauss EW, Ladman AJ, Gardner FH (1961) A morphologic and histochemical analysis of the human jejunal epithelium in non-tropical sprue. Gastroenterology 40:735–765
Philips AD, Rice SJ, France NE, Walker-Smith JA (1979) Small intestinal intraepithelial lymphocyte levels in cow's milk protein intolerance. Gut 20:509–512
Ross IN, Mathan VI (1981) Immunological changes in tropical sprue. Quart J Med 50:435–449
Rubin CE, Brandborg L, Phelps P (1960) Studies of celiac disease. I. The apparent, identical and specific nature of the duodenal and proximal jejunal lesion in coeliac disease and idiopathic steatorrhoea. Gastroenterology 38:28
Rubin W, Ross L, Sleisenger MH Weser E (1966) An electron microscope study of adult coeliac disease. Lab Invest 15:1720–1747
Shiner M, Doniach I (1960) Histopathological studies in steatorrhoea. Gastroenterology 38:419–440
Shiner M (1983) Ultrastructure of the small intestinal mucosa. Springer, Berlin Heidelberg New York
Slavin G, Sowter C, Robertson K, McDermott S, Paton K (1980) Measurement of jejunal biopsies in computer-aided microscopy. J Clin Pathol 83:254–261
Sprinz H, Sribhibhadh R, Gangarosa E, Bonyajeti C, Kundel D, Halstead S (1962) Biopsy of small bowel of Thai people. Am J Clin Pathol 38:43–51
Trier JS, Rubin CE (1965) Electron microscopy of the small intestine: a review. Gastroenterology 49:574–603
Trier JS (1968) Morphology of the epithelium of the small intestine. In: Code CF (ed) Handbook of physiology, Sec 6, vol III. The alimentary canal. Am Physiol Soc, Washington DC, pp 1125–1175
Watson AJ, Wright NA (1974) Morphology and cell kinetics of the jejunal mucosa in untreated patients. In: WT Cooke, P Asquith (eds) Coeliac disease. Saunders, London, pp 11–31
Weibel ER (1979) Stereological Methods, vol. I. Practical methods for biological morphometry. Academic Press, New York
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Supported by Medical Research Council (United Kingdom)
Part of thesis submitted to Victoria University of Manchester by N. M. Niazi
University of Manchester School of Medicine
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Niazi, N.M., Leigh, R., Crowe, P. et al. Morphometric analysis of small intestinal mucosa. Vichows Archiv A Pathol Anat 404, 49–60 (1984). https://doi.org/10.1007/BF00704250
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DOI: https://doi.org/10.1007/BF00704250