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Fucosyltransferase III and sialyl-Lex expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue

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Abstract

The potential contribution of fucosyltransferases to the overexpression of sialyl-Lex antigen was investigated in the colon carcinoma cell line HT-29 and in human colon carcinoma tissue. In HT-29 cells as well as in normal or malignant colonic tissues Fuc-TIII, Fuc-TIV, Fuc-TVI but not Fuc-TV nor Fuc-TVII were detectable after RT-PCR. Sodium butyrate treatment of HT-29 cells increased (to about 200%) and DMSO treatment decreased (to about 20%) the expression of sialyl-Lex. This modulation of sialyl-Lex was concomitant with the analogous increase/decrease of mRNA of Fuc-TIII but not Fuc-TIV. Fuc-TVI was not detectable by Northern blotting in HT-29 cells. In six human colon carcinomas which exhibited strong overexpression of sialyl-Lex, the expression of Fuc-TIII-mRNA was the same or lower than in the corresponding normal colonic tissue. Thus Fuc-TIII expression may be affecting the expression of the sialyl-Lex moiety in HT-29 cells but not in human colon carcinoma tissue.

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Authors and Affiliations

  1. Department of Gastroenterology, Klinikum Benjamin Franklin der Freien Universität Berlin, Hindenburgdamm 30, 12200, Berlin, FRG

    C. Hanski, E. Klußmann, J. Wang, C. Böhm, D. Ogorek, M. L. Hanski & E. O. Riecken

  2. Department of Dermatology, Klinikum Benjamin Franklin der Freien Universität Berlin, Hindenburgdamm 30, 12200, Berlin, FRG

    S. Krüger-Krasagakes

  3. Institute of Immunology, Klinikum Benjamin Franklin der Freien Universität Berlin, Hindenburgdamm 30, 12200, Berlin, FRG

    J. Eberle

  4. Institute of Pathology, Klinikum Benjamin Franklin der Freien Universität Berlin, Hindenburgdamm 30, 12200, Berlin, FRG

    A. Schmitt-Gräff

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  1. C. Hanski
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  10. E. O. Riecken
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Hanski, C., Klußmann, E., Wang, J. et al. Fucosyltransferase III and sialyl-Lex expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue. Glycoconjugate J 13, 727–733 (1996). https://doi.org/10.1007/BF00702336

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  • DOI: https://doi.org/10.1007/BF00702336

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