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Structure identification of natural rhamnolipid mixtures by fast atom bombardment tandem mass spectrometry

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Abstract

Two rhamnobiose-lipid preparations have been studied by fast atom bombardment (FAB) tandem mass spectrometry. The principal rhanobiose-lipids contain the β-hydroxydecanoyl-β-hydroxydecanoate Rha-Rha-C10-C10 and the β-hydroxytetradecanoyl-β-hydroxytetradecanoate Rha-Rha-C14-C14. Both preparations contain minor components which are heterogenous in β-hydroxy fatty acid composition. FAB ionization of rhamnobiose-lipids in the presence of Na+ shows the formation of both [M + Na]+, [M + 2Na - H]+, [M + 3Na - 2H]+ and [M - H]− ions. Tandem mass spectrometry of the [M + 2Na - H]+ and [M - H]− ions give information about the sequence of the building blocks. Particularly, heterogeneity in β-hydroxy fatty acid composition is determined for the principal components and all the minor components present in the preparations.

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Authors and Affiliations

  1. Bijvoet Center for Biomolecular Research, Department of Mass Spectrometry, University of Utrecht, P.O. Box 80083, 35008 TB, Utrecht, The Netherlands

    A. Manso Pajarron, C. G. de Koster, W. Heerma & J. Haverkamp

  2. Uniliver Research Laboratories, P.O. Box 114, 3130 AC, Vlaardingen, The Netherlands

    M. Schmidt & J. Haverkamp

Authors
  1. A. Manso Pajarron
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  2. C. G. de Koster
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  3. W. Heerma
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  4. M. Schmidt
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  5. J. Haverkamp
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Manso Pajarron, A., de Koster, C.G., Heerma, W. et al. Structure identification of natural rhamnolipid mixtures by fast atom bombardment tandem mass spectrometry. Glycoconjugate J 10, 219–226 (1993). https://doi.org/10.1007/BF00702203

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  • DOI: https://doi.org/10.1007/BF00702203

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