Abstract
A cell line derived from the urothelium lining the ureter of a 12-year-old girl was immortalised using a temperature-sensitive SV40 large T-antigen gene construct, and designated UROtsa. Following immortalisation, UROtsa cells expressed SV40 large T-antigen, but did not acquire characteristics of neoplastic transformation, including growth in soft agar or the development of tumours in nude mice. Metaphase spreads had a normal chromosomal appearance and number. UROtsa cells remained permissive for cell growth at 39°C, indicating that they did not retain temperature sensitivity. UROtsa provides an in vitro model of “normal” urothelium.
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Petzoldt, J.L., Leigh, I.M., Duffy, P.G. et al. Immortalisation of human urothelial cells. Urol. Res. 23, 377–380 (1995). https://doi.org/10.1007/BF00698738
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DOI: https://doi.org/10.1007/BF00698738