Summary
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1.
Catecholamines (noradrenaline, adrenaline and isoprenaline) were tested for their effect on the resting membrane potential of mouse skeletal muscle cells. In freshly isolated muscles incubated in the normal solution containing 5 mol · l−1, catecholamines increased the resting membrane potential (RMP) by 3–5 mV. In Na+-loaded muscles incubated in a K+-free solution, however, catecholamines increased the RMP by 13–16 mV; consequent application of K+ to these muscles did not hyperpolarize the membrane further. A significant decrease of input membrane resistance was observed during the noradenaline-induced hyperpolarization. This indicates that the passive membrane permeability for K+ ions was apparently increased. Noradrenaline-induced hyperpolarization requires the presence of calcium ions in the incubation solution. We therefore assume that catecholamines hyperpolarize the membrane by Ca2+-dependent K+-channels activation.
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2.
The action of catecholamines on the resting membrane potential of skeletal muscle exhibits a 50% nonspecific effect as far as the adrenergic receptor is concerned, and the rest may be blocked by adrenergic blocking agents.
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Zemková, H., Svoboda, P., Teisinger, J. et al. On the mechanism of catecholamine-induced hyperpolarization of skeletal muscle cells. Naunyn-Schmiedeberg's Arch. Pharmacol. 329, 18–23 (1985). https://doi.org/10.1007/BF00695186
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DOI: https://doi.org/10.1007/BF00695186