Summary
Peroxidase (HRP) conjugates ofArachis hypogaea agglutinin (PNA),Dolichos biflorus agglutinin (DBA),Ulex europaeus agglutinin I (UEA I) andBandeiraea simplicifolia agglutinin I (BSA I) were used to detect the expression of carbohydrate moieties in glyco-conjugates of normal human duodenal mucosa. Lectin histology was performed on sections from paraffin embedded specimens of donor blood groups A, B, AB and O. While PNA, DBA and UEA I staining appeared to be independent of the donor blood groups, BSA I affinity was restricted to the blood groups B and AB. Neither striated columnar cells nor goblet cells stained with PNA-HRP conjugates. Glyco-conjugates of goblet cell mucins reacted with DBA and UEA I, but staining intensities varied in crypts and villi: positive goblet cells occurred preferentially in upper parts of the crypts and in villi. Striated columnar cells also exhibited variation in staining intensities, with binding of DBA and UEA I observed in the upper half of crypts and in villi, occurring predominantly along the brush border and in apical parts of the cells. Glyco-conjugates synthesized by Brunner's gland cells possessed a great variety of lectin reactivity with the lectins employed. Within a given histological section positive gland areas with different staining intensities beside negative cell populations might reflect different functional states.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boland CR, Montgomery CK, Kim YS (1982) Alterations in human colonic mucin occurring with cellular differentiation and malignant transformation. Proc Natl Acad Sci (USA) 79:2051–2055
Culling CFA, Reid PE (1977) The apparent failure of sodium borohydride reduction to block further PAS reactivity in rat epithelial mucins. Histochem J 9:781–785
Culling CFA, Reid PE, Dunn WL, Freeman HJ (1981) The relevance of the histochemistry of colonic mucins based upon their PAS reactivity. Histochem J 13:889–903
Etzler ME, Kabat EA (1970) Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity from Dolichos biflorus. Biochemistry 9:869–877
Etzler ME, Branstrator ML (1974) Differential localization of cell surface and secretory components in rat intestinal epithelium by use of lectins. J Cell Biol 62:329–343
Filipe MI (1979) Mucins in the human gastrointestinal epithelium: a review. Invest Cell Pathol 2:195–216
Filipe MI, Fenger C (1979) Histochemical characteristics of mucins in the small intestine. A comparative study of normal mucosa, benign epithelial tumours and carcinoma. Histochem J 11:277–287
Freeman HJ, Lotan R, Kim YS (1980) Application of lectins for detection of goblet cell glycoconjugate differences in proximal and distal colon of the rat. Lab Invest 42:405–412
Gad A (1979a) A histochemical study of human alimentary tract mucosubstances in health and disease. I. Normal and tumours. Br J Cancer 23:52–63
Gad A (1969b) A histochemical study of human alimentary tract mucosubstances in health and disease. II. Inflammatory conditions. Br J Cancer 23:64–68
Goldman H, Ming SC (1968) Mucins in normal and neoplastic gastrointestinal epithelium. Arch Pathol 85:580–586
Gottschalk A (1963) The basic structure of glycoproteins and problems of their chemical and physicochemical analysis. Ann NY Acad Sci 106:168–176
Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Hounsell EF, Feizi T (1982) Gastrointestinal mucins. Structures and antigenicities of their carbohydrate chains in health and disease. Med Biol 60:227–236
Ito S (1969) Structure and function of the glycocalyx. Fed Proc 28:12–25
Kapadia A, Feizi T, Jewell D, Keeling J, Slavin G (1981) Immunocytochemical studies of blood group A, H, I and i antigens in gastric mucosae of infants with normal gastric histology and of patients with gastric carcinoma and chronic benign peptic ulceration. J Clin Pathol 34:320–37
Kuhlmann WD (1975) Purification of mouse alpha, -fetoprotein and preparation of specific peroxidase conjugates for its cellular localization. Histochemistry 44:155–167
Kuhlmann WD, Peschke P, Wurster K (1983) Lectin-peroxidase conjugates in histopathology of gastrointestinal mucosa. Virchows Arch [Pathol Anat] 398:319–328
Lev R, Spicer SS (1964) Specific staining of sulphate groups with Alcian blue at low pH. J Histochem Cytochem 12:309
Lev R, Spicer SS (1965) A histochemical comparison of human epithelial mucins in normal and in hypersecretory states including pancreatic cystic fibrosis. Am J Pathol 46:23–48
Lotan R, Skutelsky E, Danon D, Sharon N (1975) The purification, composition, and specificity of the Anti-T lectin from peanut (Arachis hypogaea). J Biol Chem 250:8518–8523
Matsumoto I, Osawa T (1969) Purification and characterization of an Anti-H(0) phytohemagglutinin of Ulex europaeus. Biochim Biophys Acta 194:180–189
Morson BC, Dawson IMP (1979) Gastrointestinal Pathology. Blackwell, Oxford London
Murphy LA, Goldstein IJ (1977) Five α-D-Galactopyranosyl-binding isolectins from Bandeiraea simplicifolia seeds. J Biol Chem 252:4739–4742
Pearse AGE (1980) Histochemistry. Theoretical and Applied. Churchill-Livingstone, London
Sato A, Spicer SS (1982) Ultrastructural visualization of galactosyl residues in various alimentary epithelial cells with the peanut lectin-horseradish peroxidase procedure. Histochemistry 73:607–624
Sheahan DG, Jervis HR (1976) Comparative histochemistry of gastrointestinal mucosubstances. Am J Anat 146:103–132
Slocombe GW, Berry CL, Swettenham KV (1980) The variability of blood group antigens in gastric carcinoma as demonstrated by the immunoperoxidase technique. Virchows Arch [Pathol Anat] 387:289–300
Smits HL, Van Kerkhof JM, Kramer MF (1982) Isolation and partial characterization of rat duodenal-gland (Brunner's-gland) mucus glycoprotein. Biochem J 203:779–785
Spicer SS (1965) Diamine methods for differentiating mucosubstances histochemically. J Histochem Cytochem 13:211–234
Stoward PJ, Spicer SS, Miller RL (1980) Histochemical reactivity of peanut lectin-horseradish peroxidase conjugate. J Histochem Cytochem 28:979–990
Suzuki S, Tsuyama S, Suganuma T, Yamamoto N, Murata F (1981) Postembedding staining of Brunner's gland with lectin-ferritin conjugates. J Histochem Cytochem 29:946–952
Weiser MM, Wilson JR (1981) Serum levels of glycosyltransferases and related glycoproteins as indicators of cancer: biological and clinical implications. CRC Crit Rev Clin Lab Sci 14:189–239
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wurster, K., Peschke, P. & Kuhlmann, W.D. Cellular localization of lectin-affinity in tissue sections of normal human duodenum. Vichows Archiv A Pathol Anat 402, 1–9 (1983). https://doi.org/10.1007/BF00695044
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00695044