Summary
The oxidation of 1-14C palmitate, 2-14C glucose, 1-14C lactate, 1-14C alanine, 1-14C leucine and 1-14C glutamate, injected via a cannula into the dorsal aorta, was determined in trout, either at rest, or during swimming at 80% of the maximum sustained speed. The oxygen consumption and the excretion rates of14CO2 as well as CO2 were measured.
While the oxygen consumption of swimming trout was about twice as high as of resting trout, the oxidation rates of the injected tracers increased by up to 9 time. Despite the increased importance of blood borne substrates, the estimated contribution to total CO2 production is about 6% for the resting and 17% for the active trout. The majority of the oxidisable substrates must therefore be endogenous.
The mobilization and oxidation rates of lactate, palmitate and leucine were particularly increased during swimming. During rest, palmitate and leucine oxidation rates are low. While oxidation rates of alanine and glutamate are intermediate, those of glucose were found to be extremely low, both at rest and during swimming. The measured RQ values for resting and swimming trout were 0.91 and 0.96 respectively, indicating that protein is the major fuel, since glucose oxidation seems of minor importance.
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Abbreviations
- SA:
-
specific activity
- tt:
-
transit time
- τ:
-
decay (time) constant
- ψ:
-
flux (in % of injected dose per hour)
- Ucrit :
-
critical velocity of sustained swimming
- TCO2 :
-
total CO2
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van den Thillart, G. Energy metabolism of swimming trout (Salmo gairdneri). J Comp Physiol B 156, 511–520 (1986). https://doi.org/10.1007/BF00691037
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DOI: https://doi.org/10.1007/BF00691037