Abstract
Thallium sulphate inhibited microbial growth, withBacillus megaterium KM, more sensitive to the metal thanSaccharomyces cerevisiae andEscherichia coli. Inhibition ofB. megaterium KM andS. cerevisiae, but not ofE. coli, was alleviated by increasing the potassium concentration of the medium; inhibition of respiration ofS. cerevisiae, but not ofE. coli, was similarly alleviated. Thallium was rapidly bound, presumably to cell surfaces, byS. cerevisiae andE. coli, and was progressively accumulated by energy-dependent transport systems (probably concerned primarily with potassium uptake) with both organisms. Thallium uptake kinetics suggested more than one transport system operated in yeast, possibly reflecting a multiplicity of potassium transport systems. ApparentK m andK i values for competitive inhibition of thallium uptake by potassium indicatedS. cerevisiae to have a higher affinity for thallium uptake than for potassium, whileE. coli had a transport system with a higher affinity for potassium than for thallium. The likely systems for thallium transport are discussed. A mutant ofE. coli with tenfold decreased sensitivity to thallium was isolated and apparently effected surface binding of thallium in amounts equivalent to the wild type organism, but showed no subsequent uptake and accumulation of the metal from buffer, even though it was able to accumulate potassium to normal intracellular concentrations during growth.
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Adelberg, A., Mandel, M., Chen, G. C. C.: Optimal conditions for mutagenesis by N-methyl-N-nitro-N′-nitrosoguanidine inEscherichia coli K12. Biochem. biophys. Res. Commun.18, 788–795 (1965)
Antia, N. J., Kripps, R. S., Desai, I. D.:l-Threonine deaminase in marine planktonic algae. III. Stimulation of activity by monovalent inorganic cations and diverse effects from other ions. Arch. Mikrobiol.85, 341–354 (1972)
Armstrong, W. McD.: Ion transport and related phenomena in yeast and other microorganisms. In: Transport and accumulation in biological systems (E. J. Harris, ed.), pp. 407–445. London: Butterworth 1972
Armstrong, W. McD., Rothstein, A.: Discrimination between alkali metal cations by yeast. I. Effect of pH on uptake. J. gen. Physiol.48, 61–71 (1964)
Bhattacharyya, P., Epstein, W., Silver, S.: Valinomycin-induced uptake of potassium in membrane vesicles fromEscherichia coli. Proc. nat. Acad. Sci. (Wash.)68, 1488–1492 (1971)
Conway, E. J., Beary, M. E.: Active transport of magnesium across the yeast cell membrane,. Biochem. J.69, 275–280 (1958)
Fitzgerald, G., Williams, L. S.: Modiffied penicillin enrichment procedure for the selection of bacterial mutants. J. Bact.122, 345–346 (1975)
Good, N. E., Winget G., Winter, W., Connolly T. N., Izawa, S., Singh, R. M. M.: Hydrogen ion buffers for biological research. Biochemistry5, 467–477 (1966)
Harold, F. M., Altendorf, K.: Cation transport in bacteria: K+, Na+, and H+. In: Current topics in membranes and transport, Vol. 5 (F. Bronner, A. Kleinzeller, eds.), pp. 1–50. New York: Academic Press 1974
Hughes, M. N.: The inorganic chemistry of biological processes, p. 286. London: Wiley 1972
Kunze, M.: Der Einfluß von Thalliumazetat auf das Wachstum von Acholeplasmataceae, Mycoplasmataceae und cinigen Bakterienspezies. Zbl. Bakt., I. Abt. Orig. A222, 535–539 (1972)
Lindegren, C. C., Lindegren, G.: Oxidative detoxication of thallium in the yeast mitochondria. Antonie v. Leeuwenhoek.39, 351–353 (1973)
Lineweaver, H., Burk, D.: The determination of enzyme dissociation constants. J. Amer. chem. Soc.56, 658–666 (1934)
Nelson, D. L., Kennedy E. P.: Magnesium transport inEscherichia coli: inhibition by cobaltous ion. J. biol. Chem.246, 3042–3049 (1971)
Overnell, J.: The effect of some heavy metal ions on photosynthesis in a freshwater alga. Pesticide Biochem. Physiol.5, 19–26 (1975)
Pauling, L.: The nature of the chemical bond and the structure of molecules and crystals. New York: Cornell University Press 1960
Rothstein, A.: Relationship of cation influxes and effluxes in, yeast. J. gen. Physiol.64, 608–621 (1974)
Rothstein, A., Hayes, A. D.: The relationship, of the cell surface to metabolism. XIII. The cation binding properties of the yeast cell surface. Arch. Biochem. Biophys.63, 87–99 (1956)
Ryan J. P., Ryan, H.: The role of intracellular pH in the regulation of cation exchanges in yeast. Biochem. J.128, 139–146 (1972)
Solt, J., Paschinger, H., Broda, E.: Die energieabhängige Aufnahme von Thallium durchChlorella. Planta (Berl.)101, 242–250 (1971)
Weiden, P. L., Epstein, W., Schultz, S. G.: Cation transport inEscherichia coli. VII. Potassium requirement for phosphate uptake. J. gen. Physiol.50 1641–1661 (1967)
Zitko, V., Carson, W. V.: Accumulation of thallium in clams and mussels. Bull. Environ. Contam. Toxicol.14, 530–533 (1975)
Zitko, V., Carson, W. V., Carson, W. G.: Thallium occurrence in the environment and toxicity to fish. Bull. Environ. Contam. Toxicol.13, 23–30 (1975)
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Abbreviations: Metal are referred to by their recognised atomic symbols (e.g. TI = Thallium; K = potassium; Co = cobalt)
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Norris, P., Man, W.K., Hughes, M.N. et al. Toxicity and accumulation of thallium in bacteria and yeast. Arch. Microbiol. 110, 279–286 (1976). https://doi.org/10.1007/BF00690239
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DOI: https://doi.org/10.1007/BF00690239