Abstract
A facultatively anaerobic spirochete isolated from a high-salinity pond grew optimally when 0.75 M NaCl, 0.2 M MgSO4, and 0.01 M CaCl2 were present in media containing yeast extract, peptone, and a carbohydrate. The organism failed to grow when any one of these three salts was omitted from the medium. Aerobically-grown colonies of the spirochete were red, whereas anaerobically-grown colonies showed no pigmentation. Non-pigmented mutants of the spirochete were isolated.
The spirochete used carbohydrates, but not amino acids, as energy sources. Glucose was fermented to CO2, H2, ethanol, acetate, and a small amount of lactate. Determinations of radioactivity in products formed from glucose-1-14C and enzymatic assays indicated that glucose was dissimilated to pyruvate mainly via the Embden-Meyerhof pathway. Pyruvate was metabolized through a clostridial-type clastic reaction.
Cells growing acrobically performed an incomplete oxidation of glucose mainly to CO2 and acetate. Comparison of aerobic and anaerobic growth yields indicated that oxidative phosphorylation occurred in cells growing aerobically. The guanine + cytosine content of the DNA of the spirochete was 62 moles%. It is proposed that the spirochete described herein be considered a new species and that it be namedSpirochaeta halophila.
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References
Bayley, S. T., Kushner, D. J.: The ribosomes of the extremely halophilic bacterium,Halobacterium cutirubrum. J. molec. Biol.9, 654–669 (1964)
Breznak, J. A., Canale-Parola, E.:Spirochaeta aurantia, a pigmented, facultatively anaerobic spirochete. J. Bact.97, 386–395 (1969)
Breznak, J. A., Canale-Parola, E.: Metabolism ofSpirochaeta aurantia. I. Anaerobic energy-yielding pathways. Arch. Mikrobiol.83, 261–277 (1972a)
Breznak, J. A., Canale-Parola, E.: Metabolism ofSpirochaeta aurantia. II. Aerobic oxidation of carbohydrates. Arch. Mikrobiol.83, 278–292 (1972b)
Breznak, J. A., Canale-Parola, E.: Morphology and physiology ofSpirochaeta aurantia strains isolated from aquatic habitats. Arch. Microbiol.105, 1–12 (1975)
Buchanan, R. E., Gibbons, N. E. (eds.): Bergey's manual of determinative bacteriology, 8th ed. Baltimore: Williams and Wilkins 1974
Canale-Parola, E., Holt, S. C., Udris, Z.: Isolation of free-living, anaerobic spirochetes. Arch. Mikrobiol.59, 41–48 (1967)
Canale-Parola, E., Udris, Z., Mandel, M.: The classification of free-living spirochetes. Arch. Mikrobiol.63, 385–397 (1968)
Conn, H. J., Jennison, M. W., Weeks, O. B.: Routine tests for the identification of bacteria. In: Manual of microbiological methods (M. J. Pelczar, H. J. Conn, eds.), pp. 140–168. New York: McGraw-Hill 1957
DeLey, J.: Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bact.101, 738–754 (1970)
Eckstein, Y.: Physicochemical limnology and geology of a meromictic pond on the Red Sea shore. Limnol. Oceanogr.15, 363–372 (1970)
Greenberg, E. P., Canale-Parola, E.: Carotenoid pigments of facultatively anaerobic spirochetes. J. Bact.123, 1006–1012 (1975)
Hespell, R. B., Canale-Parola, E.: Carbohydrate metabolism inSpirochaeta stenostrepta. J. Bact.103, 216–226 (1970a)
Hespell, R. B., Canale-Parola, E.:Spirochaeta litoralis sp. n., a strictly anaerobic marine spirochete. Arch. Mikrobiol.74, 1–18 (1970b)
Hespell, R. B., Joseph, R., Mortlock, R. P.: Requirement for coenzyme A in the phosphoroclastic reaction of anaerobic bacteria. J. Bact.100, 1328–1334 (1969)
Holdeman, L. V., Moore, W. E. C.: Anaerobe laboratory manual. V.P.I. Anaerobe Laboratory, Virginia Polytechnic Institute and State Univ. Virginia (1972)
Joseph, R., Canale-Parola, E.: Axial fibrils of anaerobic spirochetes: Ultrastructural and chemical characteristics. Arch. Mikrobiol.81, 146–168 (1972)
Koepsell, H. J., Johnson, M. J.: Dissimilation of pyruvic acid by cell-free preparations ofClostridium butylicum. J. biol. Chem.145, 379–386 (1942)
Krumbein, W. E., Cohen, Y.: Biogene, klastische und evaporitische Sedimentation in einem mesothermen monomiktischen ufernahen See (Golf von Aqaba). Geol. Rundschau63, 1035–1065 (1974)
Kushner, D. J.: Halophilic bacteria. Advanc. appl. Microbiol.10, 73–97 (1968)
Lanyi, J. K.: Salt-dependent properties of proteins from extremely halophilic bacteria. Bact. Rev.38, 272–290 (1974)
Larsen, H.: Halophilism. In: The bacteria, Vol. IV (I. C. Gunsalus, R. Y. Stanier, eds.), pp. 297–342. New York: Academic Press 1962
Larsen, H.: The halobacteria's confusion to biology. Antonie v. Leeuwenhoek39, 383–396 (1973)
MacLeod, R. A.: On the role of inorganic ions in the physiology of marine bacteria. Advanc. Microbiol. Sea1, 95–126 (1968)
Reichelt, J. L., Baumann, P.: Effect of sodium chloride on growth of heterotrophic marine bacteria. Arch. Microbiol.97, 329–345 (1974)
Stadtman, E. R., Novelli, G. D., Lipmann, F.: Coenzyme A function in and acetyl transfer by the phosphotransacetylase system. J. biol. Chem.191, 365–376 (1951)
Stouthamer, A. H.: Determination and significance of molar growth yields. In: Methods in microbiology, Vol. I (I. R. Norris, D. W. Ribbons, eds.), pp. 629–663. New York: Academic Press 1969
Umbreit, W. W., Burris, R. H., Stauffer, J. F.: Manometric techniques. Minneapolis, Minn.: Burgess 1964
Wolfe, R. S., O'Kane, D. J.: Cofactors of the phosphoroclastic reaction ofClostridium butyricum. J. biol. Chem.205, 755–765 (1953)
Wolfe, R. S., O'Kane, D. J.: Cofactors of the carbon dioxide exchange reaction ofClostridium butyricum. J. biol. Chem.215, 637–643 (1955)
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Greenberg, E.P., Canale-Parola, E. Spirochaeta halophila sp. n., a facultative anaerobe from a high-salinity pond. Arch. Microbiol. 110, 185–194 (1976). https://doi.org/10.1007/BF00690227
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DOI: https://doi.org/10.1007/BF00690227