Summary
In European woodmice the amount and intensity of daily activity was compared to oxygen uptake and to the potential for oxidative metabolism of heart and skeletal muscle. One group of animals was inactivated by exposition to light during night time; another group of animals was trained by enforced running on a treadmill. The oxidative potential of the muscle tissue was assessed by morphometry of capillaries and mitochondria. A novel sampling technique was used which allowed us to obtain morphological data related to single muscles, to muscle groups, and finally to whole body muscle mass.
Reducing the spontaneous activity by ten fold had no effect on oxygen uptake nor on capillaries or mitochondria in locomotory muscles. Mitochondrial volume was reduced, however, in heart and diaphragm. Enforced running increased the weight specific maximal oxygen uptake significantly. It also increased the mitochondrial volume in heart and diaphragm as well as in M. tibialis anterior. Capillary densities were neither affected by training nor by inactivation. A significant correlation was found between the capillary density and the volume density of mitochondria in all muscles analysed morphometrically. For the whole skeletal muscle mass of a European woodmouse the inner mitochondrial membranes were estimated to cover 30 m2. The oxygen consumption per unit time and per unit volume of muscle mitochondrion was found to be identical in all groups of animals (4.9 ml O2 min−1 cm−3).
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Abbreviations
- S v :
-
(im,m) surface area of inner mitochondrial membranes per unit mitochondrial volume
- V v :
-
(mt, f) volume density of mitochondria (mitochondrial volume per fiber volume)
- V (mt):
-
total mitochondrial volume
- V (f):
-
muscle volume
- N A (c, f):
-
capillary density
- ā (f):
-
mean fiber cross-sectional area
References
Andersen P (1975) Capillary density in skeletal muscle of man. Acta Physiol Scand 95:203–205
Andersen P, Henriksson J (1977) Capillary supply of the quadriceps femoris muscle of man: adaptive response to exercise. J Physiol (London) 270:677–690
Åstrand P, Rodahl K (1977) Textbook of Work Physiology. New York, McGraw Hill, pp 304
Baldwin KM, Cooke DA, Cheadle WG (1977) Time course of adaptation in cardiac and skeletal muscle to different running programs. J Appl Physiol 42:267–272
Booth FW (1982) Effect of limb immobilization on skeletal muscle. J Appl Physiol 52:1113–1118
Bylund AC, Bjurö T, Cederblad G, Holm J, Lundholm K, Aengquist KA, Sjöström M, Schersten T (1977) Physical training in man. Skeletal muscle metabolism in relation to muscle morphology and running ability. Eur J Appl Physiol 36:151–169
Cochran WG (1977) Sampling techniques. 3rd edn. Wiley, New York
Davies KJ, Packer L, Brooks GA (1981) Biochemical adaptation of mitochondria, muscle, and whole-animal respiration to endurance training. Arch Biochem Biophys 209:539–554
Davies KJ, Packer L, Brooks GA (1982) Exercise bioenergetics following sprinting training. Arch Biochem Biophys 215:260–265
Depocas F, Hart JS (1957) Use of the Pauling oxygen analyzer for measurement of oxygen consumption of animals in open-circuit system and in short lag, closed-circuit apparatus. J Appl Physiol 10:388–392
Henriksson J, Reitman JS (1977) Time course of changes in human skeletal muscle succinate dehydrogenase and cytochrome oxidase activities and maximal oxygen uptake with physical activity and inactivity. Acta Physiol Scand 99:91–97
Hermansen L, Wachtlova M (1971) Capillary density in skeletal muscle in well-trained and untrained men. J Appl Physiol 30:860–863
Holloszy JO, Booth FW (1976) Biochemical adaptation to endurance exercise in muscle. Annu Rev Physiol 38:273–291
Hoppeler H, Lüthi P, Claassen H, Weibel ER, Howald H (1973) The ultrastructure of the normal human skeletal muscle. A morphometric analysis on untrained men, women and well-trained orienteers. Pflügers Arch 344:217–232
Hoppeler H, Mathieu O, Weibel ER, Krauer R, Lindstedt SL, Taylor CR (1981) The design of the mammalian respiratory system. VIII. Capillaries in skeletal muscles. Respir Physiol 44:129–150
Ingjer F (1979) Effects of endurance training on muscle fibre ATP-ase activity, capillary supply and mitochondrial content in man. J Physiol (London) 294:419–432
Méndez J, Keys A (1960) Density and composition of mammalian muscle. Metabolism 9:184–188
Oerlander J, Kiessling KH, Karlsson J, Ekblom J (1977) Low intensity training, inactivity and resumed training in sedentary men. Acta Physiol Scand 101:351–362
Oscai LB, Molé PA, Brei B, Holloszy JO (1971a) Cardiac growth and respiratory enzyme levels in male rats subjected to a running program. Am J Physiol 220:1238–1241
Oscai LB, Molé PA, Holloszy JO (1971b) Effects of exercise on cardiac weight and mitochondria in male and female rats. Am J Physiol 220:1944–1948
Pasquis P, Lacaisse A, Dejours P (1970) Maximal oxygen uptake in four species of small mammals. Respir Physiol 9:298–309
Pennycuick CJ, Rezende MA (1984) The specific power output of aerobic muscle, related to the power density of mitochondria. J Exp Biol 108:377–392
Rosenmann M, Morrisson P (1974) Maximum oxygen consumption and heat loss facilitation in small homeotherms by He−O2. Am J Physiol 226:490–495
Saltin B, Blomqvist G, Mitchell JH, Johnson RL Jr, Wildenthal K, Chapman CB (1968) Response to exercise after bed rest and after training. Circulation 38 [Suppl 7]:1–78
Schenk F (1975) Behavioural responses of the woodmouse (A. sylvaticus) to various levels of illumination. Exp Brain Res [Suppl] 23:183 (abstract)
Scheuer J, Tipton CM (1977) Cardiovascular adaptations to physical training. Annu Rev Physiol 39:221–251
Seeherman HJ, Taylor CR, Maloiy GMO, Armstrong RB (1981) Design of the mammalian respiratory system. II. Measuring maximum aerobic capacity. Respir Physiol 44:11–23
Taylor CR, Maloiy GMO, Weibel ER, Langman VA, Kamau JMZ, Seeherman HJ, Heglund NC (1981) Design of the mammalian respiratory system. III. Scaling maximum aerobic capacity to body mass: wild and domestic mammals. Respir Physiol 44:25–37
Tipton CM, Carey RA, Eastin WC, Erickson HH (1974) A submaximal test for dogs: evaluation of effects of training, detraining and cage confinement. J Appl Physiol 37:271–275
Vihko V, Salminen A, Rantamaeki J (1979) Exhaustive exercise, endurance training, and acid hydrolase activity in skeletal muscle. J Appl Physiol 47:43–50
Weibel ER (1979) Sterological methods vol 1: Practical methods for biological morphometry. Academic Press, London New York Toronto
Weibel ER, Taylor CR, Gehr P, Hoppeler H, Mathieu O, Maloiy GMO (1981) Design of the mammalian respiratory system. IX. Functional and structural limits for oxygen flow. Respir Physiol 44:151–164
Withers PC (1977) Measurement of\(\dot V_{O_2 } ,\dot V_{CO_2 } \) and evaporative water loss with a flow-through mask. J Appl Physiol 42:120–123
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Hoppeler, H., Lindstedt, S.L., Uhlmann, E. et al. Oxygen consumption and the composition of skeletal muscle tissue after training and inactivation in the European woodmouse (Apodemus sylvaticus). J Comp Physiol B 155, 51–61 (1984). https://doi.org/10.1007/BF00688791
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DOI: https://doi.org/10.1007/BF00688791