Summary
The composition and oxidative capacity of brown adipose tissue (BAT) were investigated in Djungarian hamsters kept under natural photoperiod, either indoors at neutralT a (23°C) or under outdoor conditions. BAT comprises up to 5% of the body weight in summer/indoor hamster, with lipid representing 86% of the total tissue mass. Tissue mass and thermogenic capacity are inversely related during seasonal adaptation: 30% decrease of total DNA, accompanied by extensive lipid depletion, reduces the amount of BAT by almost 60% during acclimatization from summer/indoor to winter/outdoor conditions. Mitochondrial protein in BAT is increased by a factor of 2.6 concomitantly, and by a factor of 4 when related to body weight (body weight reduction 36%).
Cytochrome oxidase activity in different brown fat deposits varies by up to 150% in summer/indoor hamsters; depending on the fat pad, the enzyme activity is increased 200%–700% during adaptation to winter/outdoor conditions.
Natural photoperiod is decisive in determining the seasonal adaptation of DNA content in BAT and of body weight. Short photoperiod alone may lead to depletion of lipid content of BAT and thus decrease the tissue mass practically to the lowest seasonal level, even though both parameters may be also influenced byT a. One third of the maximum adaptive increase of tissue mitochondria may be attributed to seasonal changes in photoperiod and up to two thirds toT a. Photoperiod establishes a fixed fundament of slow-reacting functional adaptation of BAT, whereas the effect of decreasedT a depends on the rate and duration of cold influence.
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Abbreviations
- BAT :
-
brown adipose tissue
- NST :
-
nonshivering thermogenesis
- T a :
-
ambient temperature
References
Barnard T, Skala J (1970) The development of brown adipose tissue. In: Lindberg O (ed) Brown adipose tissue. Elsevier, New York, pp 33–72
Cameron JL, Smith RE (1964) Cytological response of brown fat tissue in cold-exposed rats. J Cell Biol 23:89–100
Chaffee RRJ, Roberts JC (1971) Temperature acclimation in birds and mammals. Annu Rev Physiol 33:155–202
Feist DD, Rosenmann M (1976) Norepinephrine thermogenesis in seasonally acclimatized and cold acclimated red-backed voles in Alaska. Can J Physiol Pharmacol 54:146–153
Foster DO, Frydman M (1978) Non-shivering thermogenesis in the rat: measurements of blood flow with microspheres point to brown adipose tissue as the dominant site of calorigenesis induced by noradrenalin. Can J Physiol Pharmacol 56:110–122
Girardier L (1977) The regulation of the biological furnace of warm blooded animals. Introduction. Experientia 33:1121–1124
Heldmaier G, Hoffmann K (1974) Melatonin stimulates growth of brown adipose tissue. Nature 247:224–225
Heldmaier G, Steinlechner S (1981) Seasonal control of energy requirements for thermoregulation in the Djungarian hamster (Phodopus sungorus), living in natural photoperiod. J Comp Physiol 142:429–437
Heldmaier G, Steinlechner S, Rafael J, Vsiansky P (1981) Photoperiodic control and effects of melatonin on non-shivering thermogenesis and brown adipose tissue. Science 212:917–919
Heldmaier G, Steinlechner S, Rafael J (1982) Nonshivering thermogenesis and cold resistance during seasonal acclimatization in the Djungarian hamster. J Comp Physiol 149:1–9
Himms-Hagen J (1976) Cellular thermogenesis. Annu Rev Physiol 38:315–351
Hoffman RA, Hester RJ, Towns C (1965) Effect of light and temperature on the endocrine system of the golden hamster (Mesocricetus auratus Waterhouse). Comp Biochem Physiol 15:531–533
Hohorst HJ, Rafael J (1968) Oxydative Phosphorylierung durch Mitochondrien aus brauneum Fettgewebe. Hoppe Seylers Z Physiol Chem 349:268–270
Lynch GR (1973) Seasonal changes in themogenesis, organ weights and body composition in the white-footed mouse,Peromyscus leucopus. Oecologia 13:363–376
Nicholls DG (1979) Brown adipose tissue. Biochim Biophys Acta 549:1–22
Nicholls DG, Locke RM (1984) Thermogenic mechanism in brown fat. Physiol Rev 64:1–64
Rafael J (1983) Cytochrome oxidase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol III. Verlag Chemie, Weinheim, pp 266–273
Rafael J, Vsiansky P (1981) Quantitative determination of nucleic acids in brown and white adipose tissue. Anal Biochem 115:158–162
Rafael J, Wrabetz E (1976) Brown adipose tissue mitochondria: Recoupling caused by substrate level phosphorylation and extramitochondrial adenosine phosphates. Eur J Biochem 61:551–561
Rafael J, Klaas D, Hohorst HJ (1968) Mitochondrien aus braunem Fettgewebe: Enzyme und Atmungskettenphosphorylierung während der prä- und postnatalen Entwicklung des interscapularen Fettkörpers des Meerschweinchens. Hoppe Seylers Z Physiol Chem 349:1711–1724
Rafael J, Vsiansky P, Heldmaier G (1981) Adaptive changes in brown adipose tissue of Djungarian hamsters. Acta Univ Carolinae Biol 1979:327–330
Ricquier D, Mory G, Hemon P (1976) Effects of chronic treatments upon BAT of young rats: I. Cold exposure and hyperthyroidism. Pflügers Archiv 362:241–246
Rosenmann M, Morrison P, Feist D (1975) Seasonal changes in metabolic capacity of red-backed voles. Physiol Zool 48:303–310
Smith RE, Horwitz BA (1969) Brown fat and thermogenesis. Physiol Rev 49:330–425
Suter ER (1969) The fine structure of brown adipose tissue. I. Cold-induced changes in the rat. J Ultrastruct Res 26:216–241
Vsiansky P (1985) Jahreszeitliche Adaptation des braunen Fettgewebes im Djungarischen Hamster,Phodopus sungorus (Pallas). Doctoral thesis, University Heidelberg
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Rafael, J., Vsiansky, P. & Heldmaier, G. Seasonal adaptation of brown adipose tissue in the Djungarian hamster. J Comp Physiol B 155, 521–528 (1985). https://doi.org/10.1007/BF00684683
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DOI: https://doi.org/10.1007/BF00684683