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Chromosome 11q13 markers and D-type cyclins in breast cancer

  • Molecular Genetics in Breast Cancer — minisymposium
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Summary

One in six primary human breast cancers has DNA amplification centered on the cyclin D1 gene (CCND1) on chromosome 11q13. This genetic abnormality is preferentially associated with estrogen-receptor positive tumors and may define a sub-class of patients with an adverse prognosis. AlthoughCCND1 has the credentials of a cellular oncogene, being a target for chromosomal translocation and retroviral integration, the 11q13 amplicon encompasses several other markers andCCND1 is not the only candidate for the key gene on the amplified DNA. To assess their relative importance, we have constructed a physical map of the amplified DNA and compared the extent and frequency of amplification across the region. Since it is likely that the gene providing the selective force for amplification will be expressed at elevated levels, we have also examined expression of both RNA and protein. By these criteria, cyclin D1 remains the strongest candidate for the key oncogene on the amplicon and we are currently investigating the functional consequences of its over-expression.

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Authors and Affiliations

  1. Molecular Oncology Laboratories, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, WC2A 3PX, London, United Kingdom

    Gordon Peters & Sharon Brookes

  2. Viral Carcinogenesis Laboratories, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, WC2A 3PX, London, United Kingdom

    Vera Fantl, Rosalind Smith & Clive Dickson

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Peters, G., Fantl, V., Smith, R. et al. Chromosome 11q13 markers and D-type cyclins in breast cancer. Breast Cancer Res Tr 33, 125–135 (1995). https://doi.org/10.1007/BF00682720

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