Summary
Postpartum Wistar inbred rats (weaned on the 9th puerperal day) were injected intraductally in one mammary gland with 7,12-dimethylbenze (a) anthracene (DMBA) to selectively induce ductal carcinoma. The incidence of ductal hyperplasia increased with time until it peaked at 7 weeks (12/13 animals) and then decreased. Ductal carcinoma first developed at 9 weeks in 3/12 (2 non-invasive and 1 invasive lesion) and the incidence increased with time until invasive ductal tumors were observed in 9/11 at 20 weeks. Tumors developed only in the DMBA-treated mammary glands and no systemic effects of the carcinogen were observed. Degeneration and detachment of epithelioglandular cells were seen here and there in the ducts and terminal ducts, and epithelioglandular cells proliferated in terminal duct until 2 weeks. Residual trace DMBA powder was detected in terminal ducts and the epithelioglandular layer until 7 weeks. This trace DMBA was considered to be the cause of the development of atypical epithelial cells, inducing ductal carcinomas.
Similar content being viewed by others
References
Welsch CW: Host factors affecting the growth of carcinogen-induced rat mammary carcinomas: A review and tribute to Charles Brenton Hyggins. Cancer Res 45: 3415–3443, 1985
Huggins C, Grand LC, Brillantes FK: Mammary cancer induced by a single feeding of polynuclear hydrocarbons, and its suppression. Nature 189:204–207, 1961
Gruenstein M, Meranz DR, Thatcher D, Shimkin MB: Carcinogenic effects of intragastric 3-methylcholanthrene and 7,12-dimethylbenz (a) anthracene in Wistar and Sprague-Dawley rats. J Natl Cancer Inst 36: 483–502, 1966
Geyer RP, Bleisch VR, Bryant JE, Robbins AN, Saslaw IM, Stare FJ: Tumor production in rats injected intravenously with oil emulsions containing 9,10-dimethyl-1, 2-benzanthracene. Cancer Res 11:474–478, 1951
Murad TM, Von Haam E: Studies of mammary carcinoma induced by 7, 12-dimethylbenz (a) anthracene administration. Cancer Res 32: 1404–1415, 1972
Fukunishi R: Studies of tumor induction in rats with 7, 12-dimethyl-benz (a) anthracene, with special references to the morphogenesis and fine-structural changes in the target organs. Acta Path Jap 18: 51–72, 1968
Sinha D, Dao TL: Site of origin of mammary tumors induced by 7, 12-dimethylbenz (a) anthracene in the rat. J Natl Cancer Inst 54: 1007–1009, 1975
Wellings SR, Jensen HM: On the origin and progression of ductal carcinoma in the human breast. J Natl Cancer Inst 50: 1111–1118, 1973
Fraser J: A study of the malignant breast by whole section and key block section methods. Surg Gynecol Obstet 45: 266–286, 1927
Bullock FD, Curtis MR: Spontaneous tumors of the rats. J Cancer 14: 1–115, 1930
Davis RK, Stevenson GT, Busch KA: Tumor incidence in normal Sprague-Dawley rats. Cancer Res 16: 194–197, 1956
Thompson SW, Huseby RA, Fox MA, Hunt RD: Spontaneous tumors in the Sprague-Dawley rat. J Natl Cancer Inst 27: 1037–1057, 1961
Ratcliffe HL: Spontaneous tumors in two colonies of rats of Wistar Institute of Anatomy and Biology. Am J Path 16: 237–254, 1940
Daniel PM, Prichard MML: Three types of mammary tumor induced in rats by feeding with DMBA. Br J Cancer 18: 513–520, 1964
Dao TL: Studies on mechanism of carcinogenesis in the mammary gland. Progr Exp Tumor Res, Homburger F (ed) 11: 235–261, Karger, Basel & N.Y., 1969
Mackenzie WF, Garner FM: Comparison of neoplasms in six sources of rats. J Natl Cancer Inst 50: 1243–1257, 1973
Shay H, Gruenstein M, Shimkin MB: Inhibition of mammary cancer in rats by a dithiocarbamoyl-hydrazine (IGI-33,828). Cancer Res 24: 998–1001, 1964
Huggins CB, Morii S: Selective adrenal necrosis and apoplexy induced by 7, 12-dimethylbenz (a) anthracene. J Exptl Med 114: 741–760, 1961
Sinha D, Dao TL: A direct mechanism of mammary carcinogenesis induced by 7, 12-dimethylbenz [a] anthracence. J Natl Cancer Inst 53: 841–846, 1974
Holtzman S, Meade M, Stone JP, Shellabarger CJ: Carcinogenic responses to chemicals applied directly to rat mammary glands in situ. Carcinogenesis 6: 769–772, 1985
Tsubura A, Shikata N, Inui T, Sakaida N, Morii S: Morphological and biological characteristics of mammary tumors induced by the direct application of DMBA powder to rat mammary glands. Virchows Archiv A Pathol Anat 414: 1–7, 1988
Dao TL, Sunderland H: Mammary carcinogenesis by 3-methylcholanthrene. I. Hormonal aspects in tumor induction and growth. J Natl Cancer Inst 23: 567–585, 1959
Beuving LJ, Faulkin LJ, De Ome KB, Bergs VV: Hyperplastic lesions in the mammary gland of Sprague-Dawley rats after 7, 12-dimethylbenz (a) anthracene treatment. J Natl Cancer Inst 39: 423–429, 1967
Medina D: Preneoplastic lesions in marine mammary cancer. Cancer Res 36: 2589–2595, 1976
Ashikari R, Huvos AG, Snyder RE, Lucas JC, Hutter RVD, McDivitt RW, Schottenfeld D: A clinicopathologic study of atypical lesions of the breast. Cancer 33: 130–137, 1974
Oakley CS, Welsch MA, Zhai Yi-Fan, Chang Chia-Cheng, Gould MN, Welsch CW: Comparative susceptibilities of athymic nude mice and severe combined immune deficient (SCID) mice to carcinomas: Enhanced transplantation efficiency of those rat mammary carcinomas that have elevated expression of neu oncogene. Int J Cancer 53: 1002–1007, 1993
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Terada, S., Uchide, K., Suzuki, N. et al. Induction of ductal carcinomas by intraductal administration of 7,12-dimethylbenz(a)anthracene in Wistar rats. Breast Cancer Res Tr 34, 35–43 (1995). https://doi.org/10.1007/BF00666489
Issue Date:
DOI: https://doi.org/10.1007/BF00666489