Skip to main content
SpringerLink
Log in

Metabolism of breast cancer cells as revealed by non-invasive magnetic resonance spectroscopy studies

  • Published:
Breast Cancer Research and Treatment Aims and scope Submit manuscript

Abstract

The basis for the use of nuclear magnetic resonance (NMR) spectroscopy as a tool to study the metabolism of breast cancer cells is described. The differences between proton (1H), carbon (13C), and phosphorus (31P) NMR methods is explained, and the techniques of cell extracts, cell suspensions and perfusion methods for cells are detailed. In order to perfuse cells they are preferably trapped in a gel matrix, either in the form of a thread or a bead. The gel must have appropriate properties that enables efficient oxygenation and availability of nutrients and drugs. The metabolic effects of perfusion of breast cancer cells with nutrients, drugs, and hormones are reported, and the clinical relevance of these results and methods are outlined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

NMR:

nuclear magnetic resonance

MDR:

multidrug-resistance

PME:

phosphomonoesters

PDE:

phosphodiesters

PC:

phosphocholine

PE:

phosphoethanolamine

GPC:

glycerophosphocholine

GPE:

glycerophosphoethanolamine

PCr:

phosphocreatine

2-DG-2:

deoxyglucose

EGF:

epidermal growth factor

CPD:

choline-cytidine-diphosphocholine

CDP:

ethanolamine, cytidine-diphosphoethanolamine

MRI:

magnetic resonance imaging

References

  1. Cohen JS, Knop RH, Navon G, Foxall D: Life Chem Rep 1: 281, 1983

    Google Scholar 

  2. Morris PG: Ann Rep NMR Spectrosc 20: 1–60, 1988

    Google Scholar 

  3. Daly PF, Cohen JS: Cancer Res 49: 770, 1989

    Google Scholar 

  4. Moon RB, Richards JH: J Biol Chem 248: 7276, 1973

    Google Scholar 

  5. Navon G, Ogawa S, Shulman R, Yamane T: Proc Natl Acad Sci USA 74: 87, 1977

    Google Scholar 

  6. Daniels A, Williams RJP, Wright PE: Nature 261: 321, 1976

    Google Scholar 

  7. van Zijl PCM, Moonen CWT: NMR Basic Principl Progr 26: 67. Eds. Diehl P, Fluck E, Gunther H, Kosfeld R, Seelig 28: 3–52, Springer-Verlag, Berlin, 1992

    Google Scholar 

  8. Cohen JS: Mayo Clin Proc 63: 1199, 1988

    Google Scholar 

  9. Kaplan O, van Zijl PCM, Cohen JS: NMR Basic Princpl Progr 28: 3 Eds. Diehl P, Fluck E, Gunther H, Kosfeld R, Seelig, Springer-Verlag, Berlin, 1992

    Google Scholar 

  10. Gupta RK, Gupta P, Moore RD: Ann Rev Biophys Bioeng 13: 121, 1984

    Google Scholar 

  11. Malet-Martino MC, Lame F, Ialaneix JP, Palevedy C, Hollande E, Martino R: Cancer Chemother 18: 5, 1986

    Google Scholar 

  12. Legerton TL, Kanamori K, Weiss RL, Roberts JD: Biochemistry 22: 899, 1983

    Google Scholar 

  13. Brophy PJ, Hayer KM, Riddell FG: Biochem J 210: 961, 1983

    Google Scholar 

  14. Kaplan O, van Zijl PCM, Cohen JS: Biochem Biophys Res Commun 169: 383, 1990

    Google Scholar 

  15. Glonek T, Kopp SJ, Kot E, Pettigrew JW, Harrison H, Chen MM: J Neurochem 39: 1210, 1982

    Google Scholar 

  16. Evanochko WT, Sakai TT, Ng TC, Krishna NR, Kim HD, Zeidler RB, Ghanta VK, Brockman RW, Schiffer M, Braunschweiger PG, Glickson JD: Biochim Biophys Acta 805: 104, 1984

    Google Scholar 

  17. Kushnir T, Kaplan O, Askenasy N, Navon G: Mag Reson Med 10: 119, 1989

    Google Scholar 

  18. Lowry OH, Passonneau JV: A flexible system of enzymatic analysis. Academic Press, New York, 1972, p 102

    Google Scholar 

  19. Askenasy N, Kushnir T, Kaplan O, Navon G: NMR Biomed 3: 220, 1990

    Google Scholar 

  20. Folch J, Ascoli L, Less M, Meath JA, Lebaron N: J Biol Chem 191: 833, 1951

    Google Scholar 

  21. Bines SD, Tomasovic SP, Frazer JW, Boddie AW: J Surg Res 38: 546, 1985

    Google Scholar 

  22. Meneses P, Glonek T: J Lipid Res 29: 679, 1988

    Google Scholar 

  23. Shulman RG, Brown TR, Ugurbil K, Ogawa S, Cohen SM, der Hollander JA: Science 205: 160, 1979

    Google Scholar 

  24. Boddie AW, Frazer JW, Tomasevic SP, Dennis L: J Surg Res 46: 90, 1989

    Google Scholar 

  25. Cohen JS, Lyon RC, Daly PF: Methods Enzymol 48: 435, 1988

    Google Scholar 

  26. Ugurbil K, Rottenberg H, Glynn P, Shulman RG: Biochemistry 21: 1068, 1982

    Google Scholar 

  27. Balaban RS, Gadian DG, Radda GK, Wong GG: Anal Biochem 116: 450, 1981

    Google Scholar 

  28. Jentoft JE, Town CD: J Cell Biol 101: 778, 1985

    Google Scholar 

  29. Labotka RJ, Warth JA, Winecki V, Omachi A: Anal Biochem 147: 75, 1985

    Google Scholar 

  30. Navon G, Lyon RC, Kaplan O, Cohen JS: FEBS Lett 247: 86, 1989

    Google Scholar 

  31. Kaplan O, Navon G, Lyon RC, Faustino PJ, Straka EJ, Cohen JS: Cancer Res 50: 544, 1990

    Google Scholar 

  32. Lyon RC, Cohen JS, Faustino PJ, Megnin F, Myers CE: Cancer Res 48: 870, 1988

    Google Scholar 

  33. Ronen S, Degani H: Mag Reson Med 12: 274, 1989

    Google Scholar 

  34. Ronen S, Rushkin E, Degani H: Biochim Biophys Acta 1095: 5, 1991

    Google Scholar 

  35. Daly PF, Lyon RC, Faustino PJ, Cohen JS: J Biol Chem 262: 14875, 1987

    Google Scholar 

  36. Daly PF, Zugmaier G, Sandler D, Carpen M, Myers CE, Cohen JS: Cancer Res 50: 552, 1990

    Google Scholar 

  37. Jaroszewski J, Kaplan O, Cohen JS: Cancer Res 50: 6936, 1990

    Google Scholar 

  38. Ben-Horin H, Kaplan O, Navon G: Cancer Res Submitted, 1993

  39. Neeman M, Degani H: Cancer Res 49: 589, 1989

    Google Scholar 

  40. Ruiz-Cabello J, Berghmans K, Kaplan O, Lippman ME, Clarke R, Cohen JS: Cancer Res Submitted, 1993

  41. Kaplan O, Jaroszewski J, Faustino PJ, Zugmaier G, Ennis BW, Lippman MC, Cohen JS: J Biol Chem 265: 13641, 1990

    Google Scholar 

  42. Ugurbil K, Uernsey DL, Brown TR, Glynn P, Tobkes N, Edelman IS: Proc Natl Acad Sci USA 78: 4843, 1981

    Google Scholar 

  43. Foxall DL, Cohen JS, Mitchell JB: Exp Cell Res 154: 521, 1984

    Google Scholar 

  44. Daly PF, Lyon RC, Straka EJ, Cohen JS: FASEB J 2: 2596, 1988

    Google Scholar 

  45. Shankar-Narayan K, Moress EA, Chatham JC, Barker PB: NMR Biomed 3: 23, 1990

    Google Scholar 

  46. Gonzalez-Mendez, Wemmer D, Hahn G, Wade-Jardetsky N, Jardetski O: Biochim Biophys Acta 720: 274, 1982

    Google Scholar 

  47. Freyer JP: Cancer Res 48: 2432, 1988

    Google Scholar 

  48. Foxall DL, Cohen JS: J Magn Reson 52: 346, 1983

    Google Scholar 

  49. Merchant TE, Gierke LW, Meneses P, Glonek T: Cancer Res 48: 5112, 1988

    Google Scholar 

  50. Maris JM, Evans AE, McLaughlin AC, D'Angio GJ, Bolinger L, Manos H, Chance B: New Engl J Med 312: 1500, 1985

    Google Scholar 

  51. Radda GK, Oberhaensli RD, Taylor DJ: Ann NY Acad Sci 508: 300, 1987

    Google Scholar 

  52. Sijens PE, Wijrdeman HK, Moerland MA, Bakker CJG, Vermeulen JWAH, Luyten PR: Radiology 169: 615, 1988

    Google Scholar 

  53. Warburg O: Science 123: 309, 1956

    Google Scholar 

  54. Lim P-J, Blumenstein M, Mikkelsen RB: J Magn Reson 73: 399, 1987

    Google Scholar 

  55. Smith TAD, Eccles S, Ormerod MG, Tombs AJ, Titley JC, Leach MO: Br J Cancer 64: 821, 1991

    Google Scholar 

  56. van Zijl PCM, Moonen CTW, Faustino PJ, Pekar J, Kaplan O, Cohen JS: Proc Natl Acad Sci USA 88: 3228, 1991

    Google Scholar 

  57. Cohen JS, Lyon RC, Faustino PJ, Batist G, Shoemaker M, Rubalcaba E, Cowan KH: Cancer Res 46: 4087, 1986

    Google Scholar 

  58. Evelhoch JL, Keller NA, Corbett TH: Cancer Res 47: 3396, 1987

    Google Scholar 

  59. Wick AN, Drury DR, Nakada HI, Wolfe JB: J Biol Chem 224: 963, 1957

    Google Scholar 

  60. Horton RW, Meldrum BS, Bachelard HS: J Neurochem 21: 507, 1973

    Google Scholar 

  61. Bessel EM, Courtenay VD, Foster AB, Jones M, Westwood JH: Eur J Cancer 9: 463, 1973

    Google Scholar 

  62. Jain VK, Kalia UK, Gonipath PM, Naqvi S, Kucheria K: Ind J Exp Biol 17: 1320, 1979

    Google Scholar 

  63. Purohit SC, Pohlit W: Int J Radiat Oncol Biol Phys 8: 495, 1982

    Google Scholar 

  64. Kern KA, Norton JA: Surgery 102: 380, 1987

    Google Scholar 

  65. Kaplan O, Jaroszewski J, Clarke R, Fairchikd CR, Schoenlien P, Goldenberg S, Gottesman MM, Cohen JS: Cancer Res 51: 1638, 1991

    Google Scholar 

  66. Band V, Hoffer AP, Band H, Rhinehardt AP, Knapp RC, Matlin SA, Anderson DJ: Gynecol Oncol 32: 273, 1989

    Google Scholar 

  67. Modica-Napolitano JS, Aprille: Cancer Res 47: 4361, 1987

    Google Scholar 

  68. Benz C, Hollander C, Keniry M, James TL, Mitchell M: J Clin Invest 79: 517, 1987

    Google Scholar 

  69. Paggi MG, Zupi G, Fancuiulli M, Del Carlo C, Giorno S, Laudonio N, Silvestrini B, Caputo A, Floridi A: Exp Mol Pathol 47: 154, 1987

    Google Scholar 

  70. Clarke R, Dickson RB, Brunner N: Ann Oncol 1: 401, 1990

    Google Scholar 

  71. Degani H, Victor TA, Neeman M, Itzchak Y, Horowitz A, Kaye AM: Progr Cancer Res Ther 35: 378, 1988

    Google Scholar 

  72. Ruiz-Cabello J, Berghmans K, Clarke JS, Andrews P, Simpkins H, Kaplan O, Cohn JS: 10th Ann Meeting SMRM, 1991

  73. Barzilai A, Horowitz A, Geier A, Degani H: Cancer Res 67: 2919, 1991

    Google Scholar 

  74. Neeman M, Degani H: Proc Natl Acad Sci USA 86: 5585, 1989

    Google Scholar 

  75. Lyon RC, Tschudin RG, Daly PF, Cohen JS: Mag Reson Med 6: 1, 1988

    Google Scholar 

  76. Ng TC, Grundfest S, Vijayakumar S, Baldwin NJ, Majors W, Karalis I, Meaney TE, Shin KH, Thomas FJ, Tubbs R: Mag Reson Med 10: 125, 1989

    Google Scholar 

  77. Glaholm J, Leach MO, Collins DJ, Mansi J, Sharp JC, Madden A, Smith IE, McCready JR: LancetI: 1326, 1989

    Google Scholar 

  78. Degani H, Horowitz A, Itzchak Y: Radiology 161: 53, 1986

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Tel-Aviv Medical Center, Tel-Aviv, Israel

    Ofer Kaplan

  2. Cancer Pharmacology Section, Georgetown University Medical Center, Washington, DC, USA

    Ofer Kaplan & Jack S. Cohen

Authors
  1. Ofer Kaplan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jack S. Cohen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaplan, O., Cohen, J.S. Metabolism of breast cancer cells as revealed by non-invasive magnetic resonance spectroscopy studies. Breast Cancer Res Tr 31, 285–299 (1994). https://doi.org/10.1007/BF00666161

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00666161

Key words

Search

Navigation