Abstract
The mouse B-cell cell lymphoma 70Z/3 is a convenient model system in which to study the regulation of immunoglobulin synthesis. Three transcriptional activators of kappa (κ) light chain synthesis have been identified for these cells: bacterial lipopolysaccharide (LPS), interferon-γ (IFN), and interleukin-1 (IL-1). The response of the κ gene in 70Z/3 cells to LPS is mediated by increases in two transcription factors: NF-κB and OTF-2. In contrast, IFN has no effect on either of these factors in 70Z/3 cells. We have isolated by immunoselection an LPS− IFN+ variant of 70Z/3 called 1.3E2. We show here that LPS treatment of these cells causes no increase in nuclear localization of either NF-κB or OTF-2. Although they have normal levels of cytoplasmic NF-κB, it cannot be activated by LPS or by phorbol 12-myristate 13-acetate (PMA) treatment of the cells. These experiments expand the genetic dissection of the molecular pathways of activation of κ transcription in 70Z/3 cells.
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Address correspondence and offprint requests to: C. Hopkins Sibley.
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Rooney, J.W., Emery, D.W. & Sibley, C.H. 1.3E2, a variant of the B lymphoma 70Z/3, defective in activation of NF-κB and OTF-2. Immunogenetics 31, 73–78 (1990). https://doi.org/10.1007/BF00661216
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DOI: https://doi.org/10.1007/BF00661216