Abstract
Replacement of extracellular chloride by the impermeant anion isethionate, increase in extracellular osmotic strength by addition of sucrose, or exposure to the anion transport blocking agent probenecid inhibited insulin release evoked by glucose or α-ketoisocaproate in rat isolated islets. The inhibition of insulin release due to Cl− substitution was associated with a modest decrease in glucose oxidation, but no significant change in glucose-stimulated45Ca net uptake by the islets. In the isolated perfused rat pancreas, the isethionate- or sucrose-induced inhibition of insulin release was a rapid and rapidly reversible phenomenon. Chloride substitution by isethionate inhibited more severely the second than the first phase of the secretory response to glucose, and failed to affect the insulin response to gliclazide. A chemosmotic mechanism for exocytosis, as proposed for epinephrine and parathyroid hormone release, may also be involved in insulin release.
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References
Asheroft SJH (1980) Glucoreceptor mechanisms and the control of insulin release and biosynthesis. Diabetologia 18:5–15
Brown EM, Pazoles CJ, Creutz CE, Aurbach GC, Pollard HB (1978) Role of anions in parathyroid hormone release from dispersed bovine parathyroid cells. Proc Natl Acad Sci (USA) 75:876–880
Devis G, Somers G, Malaisse WJ (1977) Dynamics of calcium-induced insulin release. Diabetologia 13:531–536
Howell SL, Young DA, Lacy PE (1969) Isolation and properties of secretory granules from rat islets of Langerhans. III. Studies of the stability of the isolated beta granules. J Cell Biol 41:167–176
Hutton JC, Sener A, Herchuelz A, Valverde I, Boschero AC, Malaisse WJ (1980) The stimulus-secretion coupling of glucose-induced insulin release. XLII. Effects of extracellular pH on insulin release: their dependency on nutrient concentration. Horm Metab Res 12:294–299
Lacy PE, Finke H, Codella RC (1975) Cinematographic studies of B granule movement in monolayer culture of islet cells. Lab Invest 33:570–576
Malaisse-Lagae F, Malaisse WJ (1971) The stimulus-secretion coupling of glucose-induced insulin release. III. Uptake of45calcium by isolated islets of Langerhans. Endocrinology 88:72–80
Malaisse WJ, Orci L (1979) The role of cytoskeleton in pancreatic B-cell function. Methods and Achievements in Experimental Pathology 9:112–136
Malaisse WJ, Brisson GR, Malaisse-Lagae F (1970) The stimulus-secretion coupling of glucose-induced insulin release. I. Interaction of epinephrine and alkaline earth cations. J Lab Clin Med 76:895–902
Malaisse WJ, Malaisse-Lagae F, Brisson GR (1971) The stimulus-secretion coupling of glucose-induced insulin release. II. Interaction of alkali and alkaline earth cations. Horm Metab Res 3:65–70
Malaisse WJ, Hager DL, Orci L (1972) The stimulus-secretion coupling of glucose-induced insulin release. IX. The participation of the B-cell web. Diabetes 21:594–604
Malaisse WJ, Sener A, Mahy M (1974) The stimulus-secretion coupling of glucose-induced insulin release. XVIII. Sorbitol metabolism in isolated islets. Eur J Biochem 47:365–370
Malaisse WJ, Herchuelz A, Devis G, Somers G, Boschero AC, Hutton JC, Kawazu S, Sener A, Atwater I, Duncan G, Ribalet B, Rojas E (1978) Regulation of calcium fluxes and their regulatory roles in pancreatic islets. Ann N Y Acad Sci 307:562–582
Malaisse WJ, Hutton JC, Kawazu S, Herchuelz A, Valverde I, Sener A (1979) The stimulus-secretion coupling of glucose-induced insulin release. XXXV. The links between metabolic and cationic events. Diabetologia 16:331–341
Matthews EK (1970) Calcium and stimulus-secretion coupling in pancreatic islet cells. In: Carafoli E, Clementi F, Drabikowski W, Margreth A (eds) Calcium transport in contraction and secretion. North-Holland Publishing Company, Amsterdam, pp 203–210
Orci L (1974) A portrait of the pancreatic B-cell. Diabetologia 10:163–187
Orci L, Gabbay KH, Malaisse WJ (1972) Pancreatic beta-cell web: its possible role in insulin secretion. Science 175:1128–1130
Pazoles CJ, Pollard HB (1978) Evidence for stimulation of anion transport in ATP-evoked transmitter release from isolated secretory vesicles. J Biol Chem 253:3962–3969
Pollard HB, Tack-Goldman K, Pazoles CJ, Creutz CE, Shulman NR (1977) Evidence for control of serotonin secretion from human platelets by hydroxyl ion transport and osmotic lysis. Proc Natl Acad Sci (USA) 74:5295–5299
Sehlin J (1978) Interrelationship between chloride fluxes in pancreatic islets and insulin release. Am J Physiol 235:E501-E508
Somers G, Blondel B, Orci L, Malaisse WJ (1979) Motile events in pancreatic endocrine cells. Endocrinology 104:255–264
Somers G, Devis G, Malaisse-Lagae F, Orci L, Malaisse WJ (1980) The chemosmotic hypothesis of hormonal release: its relevance to insulin secretion. Eur J Clin Invest 2 (Nr 2, part II) 35, abstract
Van Obberghen E, Somers G, Devis G, Vaughan GD, Malaisse-Lagae F, Orci L, Malaisse WJ (1973) Dynamics of insulin release and microtubular-microfilamentous system. I. Effect of cytochalasin B. J Clin Invest 52:1041–1051
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Somers, G., Somer, A., Devis, G. et al. The stimulus-secretion coupling of glucose-induced insulin release. Pflugers Arch. 388, 249–253 (1980). https://doi.org/10.1007/BF00658490
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DOI: https://doi.org/10.1007/BF00658490