Abstract
The effect of amiloride on cell membrane potentials and intracellular Na activity (Nai) was tested in early distal tubules of the isolated perfused kidney of control and of K-adapted (high-K diet)Amphiuma. Conventional and Na-sensitive liquid ion-exchanger microelectrodes were employed to measure the peritubular cell membrane potential (PDpt), the transepithelial potential difference (PDte) and the Na electrochemical gradient across the peritubular cell membrane (E Napt ), in the absence and the presence of amiloride (1·10−4mol·1−1) in both groups of animals. Amiloride did not affect PDpt and E Napt in control animals but depolarized PDpt and E Napt by about 8 mV in K-adapted animals. Nai (11.0 ±0.6 mmol·l−1 in early distal cells of control animals) did not change significantly by this maneuver. However, Nai decreased to extremely low values (2.3±0.2 mmol·l−1) when the luminal cotransport system for Na, Cl and K was inhibited by the luminal application of furosemide (5·10−5 mol/l) and when the luminal cell membrane was exposed simultaneously to amiloride. The amiloride-induced effects on PDpt, E Napt and Nai occurred within seconds and were fully reversible.
We conclude that high-K diet (K adaptation) induces an amiloride-sensitive pathway in the luminal cell membrane of early distal cells ofAmphiuma which exists in parallel with the furosemide-sensitive cotransport system located in this cell barrier. The results suggest a luminal amiloride-sensitive Na/H exchange mechanism which regulates the luminal K permeability.
Similar content being viewed by others
References
Aickin CC, Thomas RC (1977) An investigation of the ionic mechanism of intracellular pH regulation in mouse soleus muscle fibers. J Physiol 273: 295–316
Benos DJ (1982) Amiloride: a molecular probe of sodium transport in tissues and cells. Am J Physiol 242: C131-C145
Boron WF, Boulpaep EL (1983) Intracellular pH regulation in the renal proximal tubule of the salamander: Na−H exchange. J Gen Physiol 81: 29–52
Burg MB, Green N (1973) Function of the thick ascending limb of Henle's loop. Am J Physiol 224: 659–668
Cohen B, Giebisch G, Hansen LL, Teuscher U, Wiederholt M (1983) Relationship between peritubular membrane potential and net fluid reabsorption in the distal renal tubule ofAmphiuma. J Physiol (submitted)
Crabbé J (1972) The mechanism of action of aldosteron. From: Receptors and mechanism of action of steroid hormones, part II, 10: 513–568
Cuthbert AW, Shum WK (1976) Induction of transporting sites in a sodium transporting epithelium. J Physiol 260: 213–235
Davis CW, Finn AL (1982) Sodium transport inhibition by amiloride reduces basolateral membrane potassium conductance in tight epithelia. Science 216: 525–527
Doucet A, Katz AI (1980) Renal potassium adaptation: Na−K-ATPase activity along the nephron after chronic potassium loading. Am J Physiol 238: F380-F386
Ericson AC, Spring KR (1982) Volume regulation by Necturus gallbladder: apical Na+−H+ and Cl−−HCO −3 exchange. Am J Physiol. Am J Physiol 243: C146-C150
Friedman PA, Andreoli TE (1982) CO2-stimulated NaCl absorption in the mouse renal cortical thick ascending limb of Henle. Evidence for synchronous Na+/H+ and Cl−/HCO −3 exchange in apical plasma membranes. J Gen Physiol 80: 683–711
Frizzell RA, Schultz SG (1978) Effect of aldosterone on ion transport by rabbit colon in vitro. J Membr Biol 39: 1–26
Fromm M, Hegel U (1978) Segmental heterogeneity of epithelial transport in rat large intestine. Pflügers Arch 378: 71–83
Giebisch F (1979) Renal potassium transport. In: Giebisch G, Tosteson D, Ussing HH (eds) Transport across biological membranes. Springer, Berlin Heidelberg New York
Greger R (1981) Chloride reabsorption in the rabbit cortical thick ascending limb of Henle — a sodium dependent process. pflügers Arch 390: 38–43
Greger R, Schlatter E, Lang F (1983) Evidence for electroneutral chloride cotransport in the cortical thick ascending limb of Henle's loop for rabbit kidney. Pflügers Arch 396: 308–314
Greger R, Schlatter E (1983a) Properties of the lumen membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 396: 325–334
Greger R, Schlatter E (1983b) Properties of the basolateral membrane of the cortical thick ascending of Henle's loop of rabbit kidney. A model for secondary active chloride transport. Pflügers Arch 396: 315–324
Guggino WB, Oberleithner H, Giebisch G (1982) Relationship between cell volume and ion transport in the diluting segment of Amphiuma kidney. 15th Ann Meeting, Am Soc Nephrol, Chicago, A163
Hansen L, Teuscher U, Giebisch G, Wiederholt M (1975) Influence of luminally administered amiloride, ouabain, and amphotericin B on peritubular membrane potential and net volume reabsorption in the distal tubule. Pflügers Arch 359: R123
Kaissling B, Le Hir M (1982) Distal tubular segments of the rabbit kidney after adaptation to altered Na- and K-intake. I. Structural changes. Cell Tissue Res 224: 469–492
Katz AI (1982) Renal Na−K-ATPase: its role in tubular sodium and potassium transport. Am J Physiol 242: F207-F219
Kinsella JL, Aronson PS (1981) Amiloride inhibition of the Na+−H+ exchanger in renal microvillus membrane vesicles. Am J Physiol 241: F374-F379
Kregenow FM (1981) Osmoregulatory salt transporting mechanisms: Control of cell volume in anisotonic media. Ann Rev Physiol 43: 493–505
Le Hir M, Kaissling B, Dubach UC (1982) Distal tubular segments of the rabbit kidney after adaptation to altered Na- and K-intake. II. Changes in Na−K-ATPase activity. Cell Tissue Res 224: 493–504
Ludens JH, Fanestil DD (1974) Aldosteron stimulation of acidification of urine by isolated urinary bladder of the Colombian toad. Am J Physiol 226: 1321–1326
Marver D, Kokko JP (1983) Renal target sites and the mechanism of action of aldosterone. Mineral Electrolyte Metab 9: 1–18
Nagel W, Crabbé J (1980) Mechanism of action of aldosterone on active sodium transport across toad skin. Pflügers Arch 385: 181–187
Oberleithner H, Guggino W, Giebisch G (1981) The cellular mechanism of potassium adaptation in the distal amphibian nephron. J Physiol 318: 55p
Oberleithner H, Guggino W, Giebisch G (1982) Mechanism of distal tubular chloride transport inAmphiuma kidney. Am J Physiol 242: F331-F339
Oberleithner H, Guggino W, Giebisch G (1983) Potassium transport in the early distal tubule of kidney. Effects of potassium adaptation. Pflügers Arch 396: 185–191
Oberleithner H, Lang F, Wang W, Deetjen P (1982) Potassium (K+)-adaptation affects chloride (Cl−) reabsorption in the diluting segment ofAmphiuma. Nieren- und Hochdruckkrankheiten 5: 180
Oberleithner H, Lang F, Wang W, Giebisch G, (1982) Effect of inhibition of chloride transport on intracellular sodium activity in distal amphibian nephron. Pflügers Arch 394: 55–60
Oberleithner H, Lang F, Wang W, Messner G (1983) Evidence for stimulation of Na/H-exchange by K-adaptation in frog diluting segment. 16th Ann Meeting, Am Soc Nephrol, Washington, (in press)
Oberleithner H, Guggino W, Giebisch G (1983) The effect of furosemide on luminal sodium, chloride and potassium transport in the early distal tubule ofAmphiuma kidney. Effects of potassium adaptation. Pflügers Arch 396: 27–33
Oberleithner H, Lang F, Greger R, Wang W, Giebisch G (1983) Effect of luminal potassium on cellular sodium activity in the early distal tubule ofAmphiuma kidney. Pflügers Arch 396: 34–40
Oberleithner H, Ritter M, Lang F, Guggino W (1983) Anthracene-9-carboxylic acid inhibits renal chloride reabsorption. Pflügers Arch 398: 172–174
Petty KJ, Kokko JP, Marver DC (1981) Effects of amiloride on the acute rise in aldosterone-dependent Na−K-ATPase activity in single isolated rabbit cortical collecting tubule. Abstracts Int Congr Nephrology, Athens, TT-073
Pfaller W, Fischer WM, Strieder N, Wurnig H, Deetjen P (1974) Morphologic changes of cortical nephron cells in potassium-adapted rats. Lab Invest 31: 678–684
Schwartz GJ (1981) Na+-dependent H+ efflux from proximal tubule: evidence for reversible Na+−H+ exchange. Am J Physiol 241: F380-F385
Stanton B, Biemesderfer D, Wade J, Giebisch G (1981) Structural and functional study of the rat distal nephron: effect of potassium adaptation and depletion. Kidney Int 19: 36–48
Stanton BA, Giebisch GH (1982) Potassium transport by the renal distal tubule: effects of potassium loading. Am J Physiol 243: F487-F493
Steiner RA, Oehme M, Ammann D, Simon W (1979) Neutral carrier sodium ion-selective microelectrode for intracellular studies. Anal Chem 51: 351–353
Stoner LC (1977) Isolated perfused amphibian renal tubules: The diluting segment. Am J Physiol 233: F438-F444
Ullrich KJ, Cassola AC, Papavassiliou F, Frömter E, Hopfer U (1982) Induction of amiloride-sensitive Na+-transport on the proximal convolution of the rat kidney by low Na+ diet and aldosterone. IVth Europ Coll Renal Physiol, Prague, p 127
Vetter W, Vetter H, Siegenthaler W (1973) Radioimmunoassay for aldosterone without chromatography. 2. Determination of plasma aldosterone. Acta Endocrinol 74: 558
Weinman SA, Reuss L (1982) Na+−H+ exchange at the apical membrane of Necturus gallbladder. J Gen Physiol 80: 299–321
Wiederholt M, Hansen LL (1980)Amphiuma kidney as a model for distal tubular transport studies. Contr Nephrol 19: 28–32
Wiederholt M, Sullivan WH, Giebisch G (1971) Potassium and sodium transport across single distal tubules ofAmphiuma. J Gen Physiol 57: 495–525
Will PC, Lebowitz JL, Hopfer U (1980) Induction of amiloride-sensitive sodium transport in the rat colon by mineralocorticoids. Am J Physiol 238: F261-F268
Author information
Authors and Affiliations
Additional information
This work was supported by Österr. Forschungsrat, Proj. No.:4366
Rights and permissions
About this article
Cite this article
Oberleithner, H., Lang, F., Wang, W. et al. Evidence for an amiloride sensitive Na+ pathway in the amphibian diluting segment induced by K+ adaptation. Pflugers Arch. 399, 166–172 (1983). https://doi.org/10.1007/BF00656710
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00656710