Abstract
Rhizobium leguminosarum biovarviceae strain TAL 1236 growing on different organic P compounds as sources of phosphate exhibited phosphatase activities. The strain was able to produce both acid and alkaline phosphatase. However, its ability to produce alkaline phosphatase was much higher. When cellular phosphate fell to 0.115% of cell protein, cellular and extracellular phosphatase activities were enhanced. Mg2+, Co2+, and Ca2+ stimulated the activity of alkaline phosphatase more than acid phosphatase. However, Mn2+ and Fe2+ activated acid phosphatase rather than alkaline phosphatase. It may be concluded thatR. leguminosarum contributes significantly to the release of P from organic compounds through the action of phosphatase which can be activated by a range of cations.
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References
Abd-Alla MH (1994) Solubilization of rock phosphates byRhizobium andBradyrhizobium. Fol Microbiol 39:53–56
Alexander M (1977) Introduction soil microbiology. Wiley, New York
Belfield A, Goldberg GM (1971) Revised assay for serum phenylphosphatase activity using 4-amino antipyrine. Enzyme 12:561–573
Bhat KKS, Ney PH (1974) Diffusion of phosphate to plant roots, III. Depletion of phosphate around onion roots without root hairs. Plant and Soil 41:383–394
Bieleski RL (1973) Phosphate pools, phosphate transport and phosphate availability. Annu Rev Plant Physiol 24:225–252
Chen PS, Toribara TY, Warner H (1956) Microdetermination of phosphorus. Anal Chem 28:1756–1758
Coleman JE (1987) Multinuclear nuclear magnetic resonance approaches to the structure and mechanisms of alkaline phosphatase. In: Torriani-Gorrinia A, Rothman FG, Silver S, Wright AG, Yagil E (eds) Phosphate metabolism and cellular regulation in microorganisms. Am Soc Microbiol, Washington, DC, pp 127–138
Cosgrove DJ (1967) Metabolism of organic phosphates in soil. In: Mclaren AD, Peterson GH (eds) Soil biochemistry, vol. 1. Dekker, New York, pp 216–228
Helal HM, Sauerbeck DR (1984) Influence of plant root on C and P metabolism in soil. Plant and Soil 76:175–182
Irving GCJ, Cosgrove DJ (1971) Inositol phosphatases of microbiological origin. Some properties of a partly purified bacterial (Pseudomonas) phytase. Aust J Biol Sci 24:549–557
Irving GCJ, Cosgrove DJ (1974) Inositol phosphatases of microbiological origin. Some properties of the partly purified phytase ofAspergillus ficum. Aust J Biol Sci 24:549–557
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 183:265–275
Reid RW, Wilson JB (1971)Escherichia coli alkaline phosphatases. In: Boyer PK (ed) The enzymes, vol. 4. Academic Press, New York, pp 373–415
Rendig VV, Taylor HM (1989) Principles of soil-plant interrelationships. McGraw-Hill, New York
Somasegaran P, Hoben HJ (1985) Methods in legume-Rhizobium technology. NIFTAL project and MIRCEN, University of Hawaii, Paia
Tarafdar JC; Chhonkar PK (1979) Phosphatase production by microorganisms isolated from diverse type of soils. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg 134:119–124
Tarafdar JC, Rao AV, Bala K (1988) Production of phosphatases by fungi isolated from desert soils. Fol Microbiol 33:453–457
Whitton BA, Grainger SLH, Hawley GRW, Simon JW (1990) Cellbound and extracellular phosphatase activities of cyanobacterial isolates. Microb Ecol 21:85–98
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Abd-Alla, M.H. Use of organic phosphorus byRhizobium leguminosarum biovarviceae phosphatases. Biol Fert Soils 18, 216–218 (1994). https://doi.org/10.1007/BF00647669
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DOI: https://doi.org/10.1007/BF00647669