Summary
Muscle fibers re commonly classified histochemically into three types by the staining intensity for myosin ATPase combined with those for metabolic enzymes. Preincubation at pH 4.6 gives rise to three staining intensities of myosin ATPase which are also used for fiber typing. The two classification systems were compared by computer analysis of the individual staining profiles of over 2,500 fibers, and found not to be equivalent. The analysis showed metabolic heterogeneity among the fiber groups distinguished according to their differences in myosin ATPase.
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References
Andersen, P., Henriksson, J.: Capillary supply of the quadriceps femoris muscle of man: Adaptive response to exercise. J. Physiol. (London)277 677–690 (1977)
Baldwin, K.M., Klinkerfuss, G.H., Teriung, R.L., Molé, P.A., Holloszy, J.O.: Respiratory capacity of white, red and intermediate muscle: Adaptive response to exercise. Am. J. Physiol.222, 373–378 (1972)
Bass, A., Brdiczka, D., Eyer, P., Hofer, S., Pette, D.: Metabolic differentiation of distinct muscle types at the level of enzymatic organization. Eur. J. Biochem.10, 198–206 (1969)
Brooke, M.H., Kaiser, K.K.: Three “myosin ATPase” systems: The nature of their pH lability and sulfhydryl dependence. J. Histochem. Cytochem.18, 670–672 (1970)
Burke, R.E., Levine, D.N. Zajac, F.E., Tsairis III, P., Engel, W.K.: Mammalian motor units: Physiological-histochemical correlation in three types in cat gastrocnemius. Science174, 709–712 (1971)
Close, R.I.: Dynamic properties of mammalian skeletal muscle. Physiol. Rev.52, 129–197 (1972)
Edgerton, V., Gerchmann, L., Carrow, R.: Histochemical changes in rat skeletal muscle after exercise. Exp. Neurol.24, 110–123 (1969)
Essén, B., Jansson, E., Henriksson, J., Taylor, A.W., Saltin, B.: Metabolic characteristics of fiber types in human skeletal muscle. Acta Physiol. Scand.95, 153–165 (1975)
Henriksson, J.: Training induced adaptation of skeletal muscle and metabolism during submaximal exercise. J. Physiol. (London)270, 661–675 (1977)
Holloszy, J.O.: Biochemical adaptations to exercise: Aerobic metabolism. Exerc. Sport Sci. Rev.1, 45–71 (1973)
Khan, M.A.: Histochemical characteristics of vertebrate striated muscle: A review. Prog. Histochem. Cytochem.8, 1–48 (1976)
Kugelberg, E.: Adaptive transformation of rat soleus motor units during growth. J. Neurol. Sci.27, 269–289 (1976)
Martin, L., Engel, W.K.: Dependency of histochemical phosphorylase staining on amount of cellular glycogen. J. Histochem. Cytochem.20, 476–479 (1972)
Müller, W.: Temporal progress of muscle adaptation to endurance training in hindlimb muscle of young rats. A histochemical and morphometrical study. Cell Tissue Res.156, 61–87 (1974)
Pearse, A.G.E.: Histochemistry, 3rd Ed. Edinburg and London: Churchill Livingstone 1972
Peter, J.B., Barnard, R.J., Edgerton, V.R., Gillespie, C.A., Stempel, K.E.: Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits. Biochemistry11, 2627–2633 (1972)
Pette, D., Bücher, T.: Proportionskonstante Gruppen in Beziehung zur Differenzierung der Enzymaktivitätsmuster von Skelettmuskeln des Kaninchens. Hoppe-Seylers Z. Physiol. Chem.331, 180–195 (1963)
Pette, D., Smith, M.E., Staudte, H.W. Vrbová, G.: Effects of long-term electrical stimulation on some contractile and metabolic characteristics of fast rabbit muscle. Pflügers Arch.338, 257–272 (1973)
Pette, D.: Some aspects of supramolecular organization of glycogenolytic and glycolytic enzymes in muscle. Acta Histochem. Suppl.14, 47–68 (1975)
Pette, D., Dölken, G.: Some aspects of regulation of enzyme levels in muscle energy-supplying metabolism. Adv. Enzyme Regul.13, 355–377 (1975)
Pette, D., Müller, W., Leisner, E., Vrbová, G.: Time dependent effects on contractile properties, fibre population, myosin light chains and enzymes of energy metabolism in intermittently and continuously stimulated fast twitch muscles of the rabbit. Pflügers Arch.364, 103–112 (1976)
Pette, D., Schnez, U.: Coexistence of fast and slow type myosin light chains in single muscle fibres during transformation as induced by long term stimulation. FEBS Lett.83, 128–130 (1977)
Prince, F.P., Hikida, R.S., Hagerman, F.C.: Human muscle fibre types in power lifters, distance runners and untrained subjects. Pflügers Arch.363, 19–26 (1976)
Sjøgaard, G., Houston, M.E., Nygaard, I., Saltin, B.: Subgrouping of fast twitch fibers in skeletal muscles of man: A critical appraisal. Histochemistry58, 79–87 (1978)
Spamer, C., Pette, D.: Activity pattern of phosphofructokinase, glyceraldehydephosphate dehydrogenase, lactate dehydrogenase and malate dehydrogenase in microdissected fast and slow fibres from rabbit psoas and soleus muscle. Histochemistry52, 201–216 (1977)
Spurway, N.C.: Objective typing of mouse ankle-extensor muscle fibres based on histochemical photometry. J. Physiol. (London)277, 47P (1978)
Sréter, F.A., Gergely, J., Salmons, S., Romanul, F.: Synthesis by fast muscle of myosin light chains characteristic of slow muscle in response to long-term stimulation. Nature (New Biol.)241, 17–19 (1973)
Staudte, H., Exner, U., Pette, D.: Effects of short-term high intensity (sprint) training on some contractile and metabolic characteristics of fast and slow muscle of the rat. Pflügers Arch.344, 159–168 (1973)
Winder, W.W., Baldwin, K.M., Holloszy, J.O.: Enzymes envolved in ketone utilization in different types of muscles: Adaptaton to exercise. Eur. J. Biochem.47, 461–467 (1974)
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Nemeth, P., Hofer, H.W. & Pette, D. Metabolic heterogeneity of muscle fibers classified by myosin ATPase. Histochemistry 63, 191–201 (1979). https://doi.org/10.1007/BF00644541
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DOI: https://doi.org/10.1007/BF00644541