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Effects of fasciolicidal and anti-cestode agents on the respiration of isolatedHymenolepis diminuta mitochondria

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Summary

A mitochondrial fraction from the cestodeHymenolepis diminuta was prepared, and the respiration of the preparation followed polarographically. Of the substrates tested, glycerol-3-phosphate was the most rapidly oxidised, but the highest respiratory control ratio (1.7) was obtained using isocitrate.

With isocitrate as substrate, the fasciolicide oxyclozanide, at low concentration (161 nM), stimulated oxygen uptake and relieved oligomycin inhibition of ADP-stimulated respiration. At higher concentrations (above 2 μM) progressive inhibition of oxygen uptake was observed.

The effects of 6 other fasciolicidal or anti-cestode agents (rafoxanide, niclosamide, 3,4,5-tribromosalicylanilide, nitroxynil, resorantel, and dichlorophen) and 2,4-dinitrophenol on the respiration of the mitochondrial preparation were examined. The compounds exhibited effects similar to those of oxyclozanide on the respiration of the preparation; their relative potencies are compared.

The possible mode of action of the compounds is discussed.

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Authors and Affiliations

  1. Biology Section, Department of Science, Oxford Polytechnic, Headington, Oxford, England

    R. E. Yorke & J. A. Turton

  2. Laboratory Animals Centre, Medical Research Council, Woodmansterne Road, SM5 4EF, Carshalton, Surrey, England

    J. A. Turton

Authors
  1. R. E. Yorke
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  2. J. A. Turton
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Yorke, R.E., Turton, J.A. Effects of fasciolicidal and anti-cestode agents on the respiration of isolatedHymenolepis diminuta mitochondria. Z. Parasitenk. 45, 1–10 (1974). https://doi.org/10.1007/BF00636524

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  • DOI: https://doi.org/10.1007/BF00636524

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