Summary
Isocitrate lyase-less (Icl−) mutants were selected from genetically defined strains ofYarrowia lipolytica to investigate the regulation of synthesis of isocitrate lyase (ICL). Eighteen mutations were localized in the geneICLI, which is most probably the structural gene of ICL in this yeast. Atrans-acting positive element (ICL2) could be identified. This gene is not linked toICL1. One mutation was detected in anotherICL gene (ICL3) which is linked to geneICL1. This mutation lowered the ICL activity incis but not intrans position to geneICLI.
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Barth, G. Genetic regulation of isocitrate lyase in the yeastYarrowia lipolytica . Curr Genet 10, 119–124 (1985). https://doi.org/10.1007/BF00636476
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DOI: https://doi.org/10.1007/BF00636476