Skip to main content
SpringerLink
Log in

Head segmentation in the embryo of the Colorado beetleLeptinotarsa decemlineata as seen with anti-en immunostaining

  • Short Communications
  • Published:
Roux's archives of developmental biology Aims and scope Submit manuscript

Abstract

Segmentation in the head of the embryo of the Colorado beetleLeptinotarsa decemlineata is described on the basis of anti-engrailed (en) immunostaining of germ band stages. Six segmental units can be identified with this technique. Three segmentalen stripes can be distinguished in the gnathal region, a weak stripe interrupted medially shows the intercalary segment rudiment, a pair of oblique stripes indicate the antennal segment, and one pair of preantennalen spots are taken to indicate a sixth segment. In the broad head lobes of the beetle the spacing of the six segmental units as demarcated byen regions is similar to that in other parts of the germ band. The results are discussed with respect to old and new data concerning the number of head segments and origin of the compound eye in insects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Chadwick R, McGinnis W (1987) Temporal and spatial distribution of transcripts from theDeformed gene ofDrosophila. EMBO J 6:779–789

    Google Scholar 

  • Chaudonneret J (1984) Evolution of the insect brain, with special reference to the so-called tritocerebron. Proceedings of the XVII International Congress of Entomology, Abstracts vol. Hamburg, p 82

  • Diederich RJ, Pattatucci AM, Kaufman TC (1991) Developmental and evolutionary implications oflabial, Deformed, andengrailed expression in theDrosophila head. Development 113:273–281

    Google Scholar 

  • Fleig R (1990)engrailed expression and body segmentation in the honeybeeApis mellifera. Roux's Arch Dev Biol 198:464–473

    Google Scholar 

  • Fleig R, Sander K (1986) The embryogenesis of the honeybeeApis mellifera L. (Hymenoptera): a SEM study. Int J Insect Morphol Embryol 15:449–462

    Google Scholar 

  • Fleig R, Walldorf U, Gehring WJ, Sander K (1992) Development of theDeformed protein pattern in the embryo of the honeybeeApis mellifera L. (Hymenoptera). Roux's Arch Dev Biol 201:235–242

    Google Scholar 

  • Jürgens G, Lehmann R, Schardin M, Nüsslein-Volhard C (1986) Segmental organization of the head in the embryo ofDrosophila melanogaster. Roux's Arch Dev Biol 195:359–377

    Google Scholar 

  • Kornberg T, Sidén I, O'Farrell PH, Simon M (1985) Theengrailed locus ofDrosophila: in situ localization of transcripts reveals compartment-specific expression. Cell 40:45–53

    Google Scholar 

  • Kukalová-Peck J (1992) The “Uniramia” do not exist: the ground plan of the Pterygota as revealed by Permian Diaphanopterodea from Russia (Insecta: Paleodictyeroidea). Can J Zool 70:236–255

    Google Scholar 

  • Miller A (1940) Embryonic membranes, yolk cells, and morphogenesis of the stoneflyPteronarcys proteus Newman. Ann Entomol Soc Am 33:437–477

    Google Scholar 

  • Nair B (1949) The embryology of ofCardina laevis Heller. Proc Indian Acad Sci part B 29:211–288

    Google Scholar 

  • Patel NH, Kornberg TB, Goodman CS (1989) Expression ofengrailed during segmentation in grasshopper and crayfish. Development 107:201–212

    Google Scholar 

  • Rohrschneider I (1968) Beiträge zur Entwicklung des Vorderkopfes und der Mundregion von Periplaneta amerikana. Zool Jahrb Abt Anat Ontog Tiere 85:537–578

    Google Scholar 

  • Schmidt-Ott U, Technau GM (1992) Expression ofen andwg in the embryonic head and brain ofDrosophila indicates a refolded band of seven segment remnants. Development 116:111–125

    Google Scholar 

  • Snodgrass RE (1935) Principles of insect morphology. McGrawHill, New York London

    Google Scholar 

  • Tamarelle M (1984) Concrete evidence for transient rudiments of 2nd antennae on the “intercalary” segment of insect embryos: Comparative survey by scanning electron microscopy inAnurida maritima Guer (Collembola: Arthropleona) andHyphandria cunea Drury (Lepidoptera: Arctiidae). Int J Insect Morphol Embryol 13:331–336

    Google Scholar 

  • Turner FR, Mahowald AP (1979) Scanning electron microscopy ofDrosophila melanogaster. III. Formation of the head and caudal segments. Dev Biol 68:96–109

    Google Scholar 

  • Wada S (1966) Topographic der Anlagenkomplexe der Cephalregion vonTachycines (Saltatoria) beim Keimstreif. Naturwissenschaften 53:414

    Google Scholar 

  • Wiesmann R (1926) Entwicklung und Organogenese der Coelomblasen vonCarausius morosus Br. In: Leuzinger H, Wiesmann R, Lehmann FE (eds) Zur Kenntnis der Anatomic und Entwicklungsbiologie der StabheuschreckeCarausius morosus Br. Fischer,Jena, pp 123–328

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Biologic I (Zoologie), Albertstr. 21 a, D-79104, Freiburg, Germany

    Richard Fleig

Authors
  1. Richard Fleig
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fleig, R. Head segmentation in the embryo of the Colorado beetleLeptinotarsa decemlineata as seen with anti-en immunostaining. Roux's Arch Dev Biol 203, 227–229 (1994). https://doi.org/10.1007/BF00636338

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00636338

Key words

Search

Navigation