Summary
Pooled plasma from patients receiving phenprocoumon anticoagulant therapy was extracted and the following substances were characterized: phenprocoumon, and its 7-hydroxy,4′-hydroxy and 6-hydroxy derivatives; they were identified by HPLC and after methylation by quartz capillary GC-MS using the electron impact and selective ion monitoring modes. This is the first occasion when phenprocoumon metabolites have been identified in plasma; they were unconjugated and in much lower concentrations (43.2 and 2 ng/ml for the 7,4′ and 6-hydroxy derivatives, respectively) than the original compound (2000 ng/ml).
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de Vries, J.X., Zimmermann, R. & Harenberg, J. Phenprocoumon metabolites in human plasma; characterization by HPLC and GC-MS. Eur J Clin Pharmacol 29, 591–594 (1986). https://doi.org/10.1007/BF00635898
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DOI: https://doi.org/10.1007/BF00635898