Abstract
Mature green leaves from tobacco (Nicotiana tabacum L.) plants were submitted to contrasting light conditions; half of each leaf was shaded (changed from 60 to 25 μmol photons· m-2 ·s-1=LL) and the other half was exposed to higher light (changed from 60 to 360 μmol·m-2· s-1=HL) for 24 h. The activity and quantity of ribulose-1,5-bisphosphate carboxylase (RuBPCase) were measured during the first 24 h in each leaf region and the variation was compared with that of small subunit (SSU)-and large subunit (LSU)-mRNA contents determined by a hybridot technique. Each leaf half responded separately to the actual light received. The activity of RuBPCase increased progressively in the HL zones and decreased in the LL zones. The RuBPCase-protein content was not significantly modified during the first 24 h but SSU-mRNA content responded very rapidly to the treatment. Within 2 h a significant difference in SSU mRNA appeared between LL and HL zones: at the end of the photoperiod the content in LL zones was approx. 25% of the initial value. The increase in the exposed zone, however, was not significant, indicating that there was a dissymmetry of the response to variation in incident white light. The LSU-mRNA contents from the same leaf extracts were totally unaffected by the light treatment. No day-night variations were noted in either SSU or LSU mRNAs in control plants.
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Abbreviations
- HL:
-
high-light irradiance
- LL:
-
lower-ligh irradiance
- LSU:
-
large subunit of RuBPCase
- RuBPCase:
-
ribulose-1,5-bisphosphate carboxylase
- SSU:
-
small subunit of RuBPCase
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Prioul, JL., Reyss, A. Rapid variations in the content of the RNA of the small subunit of ribulose-1,5-bisphosphate carboxylase of mature tobacco leaves in response to localized changes in light quantity. Relationships between the activity and quantity of the enzyme. Planta 174, 488–494 (1988). https://doi.org/10.1007/BF00634477
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DOI: https://doi.org/10.1007/BF00634477