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Islet-activating protein (pertussis toxin) diminishes α2-adrenoceptor mediated effects on noradrenaline release

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Summary

The effect of islet-activating protein (IAP) on α2-adrenoceptor mediated modulation of noradrenaline release in the rabbit hippocampus was studied. Slices of the hippocampus were incubated for 6 h with IAP, subsequently loaded with3H-noradrenaline and superfused continuously. IAP-pretreatment significantly enhanced the electrically evoked transmitter release and diminished the facilitatory effect of the α2-adrenoceptor antagonist yohimbine. In addition, the inhibitory effect of the α2-adrenoceptor agonist clonidine was reduced. These results provide circumstantial evidence that an inhibitory guanine-nucleotide-binding protein, most probably N1 of a presynaptically located adenylate cyclase, is involved in the α2-autoreceptor mediated modulation of noradrenaline release.

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Abbreviations

IAP:

islet-activating protein

LPA:

leukocytosis-promoting activity

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität, Hermann-Herder-Str. 5, D-7800, Freiburg, Federal Republic of Germany

    Clemens Allgaier, Thomas J. Feuerstein, Rolf Jackisch & Georg Hertting

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  1. Clemens Allgaier
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  2. Thomas J. Feuerstein
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  3. Rolf Jackisch
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  4. Georg Hertting
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Allgaier, C., Feuerstein, T.J., Jackisch, R. et al. Islet-activating protein (pertussis toxin) diminishes α2-adrenoceptor mediated effects on noradrenaline release. Naunyn-Schmiedeberg's Arch. Pharmacol. 331, 235–239 (1985). https://doi.org/10.1007/BF00634243

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  • DOI: https://doi.org/10.1007/BF00634243

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