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Effects of carbohydrate feedings on plasma free tryptophan and branched-chain amino acids during prolonged cycling

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Summary

Brain serotonin (5-hydroxytryptamine, 5-HT) has been suggested to be involved in central fatigue during prolonged exercise. Changes in the ratio of plasma free tryptophan (free Trp) to branched-chain amino acids (BCAA) are associated with altered brain 5-HT synthesis. The purposes of this study were to describe systematically the effects of prolonged exercise on changes in plasma free Trp and BCAA and to examine the effects of carbohydrate (CHO) feedings on these same variables. Eight well-trained men [\(\dot V{\text{O}}_{\text{2}} \) max = 57.8 (SE 4.1) ml kg−1 min−1] cycled for up to 255 min at a power output corresponding toVO2 at lactate threshold (approximately 68%VO2max) on three occasions separated by at least 1 week. Subjects drank 5 ml kg−1 body wt−1 of either a water placebo, or a liquid beverage containing a moderate (6% CHO) or high (12% CHO) concentration of carbohydrate beginning at min 14 of exercise and every 30 min thereafter. Exercise time to fatigue was shorter in subjects receiving placebo [190 (SE 4) min] as compared to 6% CHO [235 (SE 10) min] and 12% CHO [234 (SE 9) min] (P<0.05). Glucose and insulin decreased in the placebo group, and free Trp, free-Trp/BCAA, and free fatty acids increased approximately five- to sevenfold (P < 0.05). These changes were attenuated in a dose-related manner by the carbohydrate drinks. Plasma free Trp and plasma free fatty acids were highly correlated (r=0.86,P<0.001). Plasma BCAA did not change in the placebo group, but decreased slightly in those receiving 6% CHO and 12% CHO (P<0.05). No differences in heart rate,\(\dot V{\text{O}}_{\text{2}} \), plasma volume and respiratory exchange ratio were found. The results indicate that free Trp and free Trp/BCAA increase progressively during prolonged cycling to fatigue. This response was attenuated by CHO feedings. Changes in plasma free fatty acids probably play a prominent role in these responses.

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References

  • Bailey SP, Davis JM, Ahlborn EA (1992) Effect of increased brain serotonergic activity on endurance performance in the rat. Acta Physiol Scand 145:75–76

    Google Scholar 

  • Blomstrand E, Celsing F, Newsholme EA (1988) Changes in plasma concentrations of aromatic and branch-chain amino acids during sustained exercise in man and their possible role in fatigue. Acta Physiol Scand 133:115–121

    Google Scholar 

  • Blomstrand E, Perrett D, Parry-Billings M, Newsholme EA (1989) Effect of sustained exercise on plasma amino acid concentrations and on 5-hydroxytryptamine metabolism in six different brain regions in the rat. Acta Physiol Scand 136:473–481

    Google Scholar 

  • Blomstrand E, Hassmen P, Ekblom B, Newsholme EA (1991a) Administration of branched-chain amino acids during sustained exercise — effects on performance and on plasma concentrations of some amino acids. Eur J Appl Physiol 63:83–88

    Google Scholar 

  • Blomstrand E, Hassmen P, Newsholme EA (1991b) Effect of branched-chain amino acid supplementation on mental performance. Acta Physiol Scand 143:225–226

    Google Scholar 

  • Bloxam DL, Hutson PH, Curzon G (1977) A simple apparatus for ultrafiltration of small volumes: application to the measurement of free and albumin-bound tryptophan in plasma. Anal Biochem 83:130–142

    Google Scholar 

  • Bloxam DL, Tricklebank MD, Patel AJ, Curzon G (1980) Effects of albumin, amino acids, and clofibrate on the uptake of tryptophan by the rat brain. J Neurochem 34:43–49

    Google Scholar 

  • Chaouloff F (1989) Physical exercise and brain monoamines: a review. Acta Physiol Scand 137:1–13

    Google Scholar 

  • Chaouloff F, Kennet GA, Serrurrier B, Merino D, Curzon G (1986) Amino acid analysis demonstrates that increased plasma free tryptophan causes the increase of brain tryptophan during exercise in the rat. J Neurochem 46:1647–1650

    Google Scholar 

  • Chaouloff F, Laude D, Merino D, Serrurrier B, Guezennec Y, Elghozi JL (1987) Amphetamine and alpha-methyl-para-tyrosine affect of exercise-induced imbalance between the availability of tryptophan and synthesis of serotonin in the brain of the rat. Neuropharmacology 26:1099–1106

    Google Scholar 

  • Coggan AR, Coyle EF (1987) Reversal of fatigue during prolonged exercise by carbohydrate infusion or ingestion. J Appl Physiol 63:2388–2395

    Google Scholar 

  • Coggan AR, Coyle EF (1988) Effect of carbohydrate feedings during high-intensity exercise. J Appl Physiol 65:1703–1709

    Google Scholar 

  • Coggan AR, Coyle EF (1991) Carbohydrate ingestion during prolonged exercise: effects on metabolism and performance. Exerc Sport Sci Rev 19:1–40

    Google Scholar 

  • Coyle EF, Coggan AR, Hopper MK, Walters TJ (1988) Determiners of endurance in well trained cyclists. J Appl Physiol 64:2622–2630

    Google Scholar 

  • Coyle EF, Hamilton MT, Gonzalez J, Montain SJ, Ivy JL (1991) Carbohydrate metabolism during intense exercise when hyperglycemic. J Appl Physiol 70:834–840

    Google Scholar 

  • Curzon G, Friedel J, Knott PJ (1973) The effect of fatty acids on the binding of tryptophan to plasma proteins. Nature 242:198–200

    Google Scholar 

  • Dill DB, Costill DL (1974) Calculation of percentages in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 37:247–248

    Google Scholar 

  • Gillmam PK, Bartlett JR, Bridges PK, Hunt A, Patel AJ, Kantamaneni BD, Curzon G (1981) Indolic substances in plasma, cerebrospinal fluid and frontal cortex of human subjects infused with saline or tryptophan. J Neurochem 37:410–417

    Google Scholar 

  • Green HJ (1991) How important is endogenous muscle glycogen to fatigue in prolonged exercise? Can J Physiol Pharmacol 69:290–297

    Google Scholar 

  • Gutman I, Wahlefeld AW (1974)l-(+)-Lactate. Determination with lactate dehydrogenase and NAD. In: Burgmeyer HU (ed) Methods of enzymatic analysis, 2nd edn. Academic Press, New York, pp 1464–1468

    Google Scholar 

  • Hyvarinen A, Nikkila EA (1962) Specific determination of blood glucose witho-toluidine. Clin Chim Acta 7:140–143

    Google Scholar 

  • MacLaren DP, Gibson H, Parry-Billings M, Edwards RTH (1979) A review of metabolic and physiological factors in fatigue. Exerc Sport Sci Rev 17:29–68

    Google Scholar 

  • MacLean DA, Spriet IL, Hultman E, Graham TE (1991) Plasma and muscle amino acid and ammonia responses during prolonged exercise in humans. J Appl Physiol 70:2095–2103

    Google Scholar 

  • Martin-Du-Pan R, Mauron C, Glaeser B, Wurtman RJ (1982) Effect of increasing oral glucose doses on plasma neutral amino acid levels. Metabolism 31:937–943

    Google Scholar 

  • McMenamy RH, Oncley JL (1958) The specific binding ofl-tryptophan to serum albumin. J Biol Chem 233:1436–1447

    Google Scholar 

  • Murray R, Siefert JG, Eddy DE, Paul GL, Halaby GA (1989) Carbohydrate feeding and exercise: effect of beverage carbohydrate content. Eur J Appl Physiol 59:152–158

    Google Scholar 

  • Newsholme EA, Acworth IN, Blomstrand E (1987) Amino acids, brain neurotransmitters and a functional link between muscle and brain that is important in sustained exercise. In: Benzi G (ed) Advances in myochemistry. John Libby Eurotext, London, pp 127–138

    Google Scholar 

  • Noma A, Okabe H, Kita M (1973) A new colormetric micro-determination of free fatty acids in serum. Clin Chim Acta 43:317–320

    Google Scholar 

  • Rayfield R (1983) REQ-200 data acquisition documentation. Rayfield Equip, Chicago, Ill

    Google Scholar 

  • Sjoggard G (1986) Water and electrolyte fluxes during exercise and their relation to muscle fatigue. Acta Physiol Scand 128[Suppl 556]:129–136

    Google Scholar 

  • Stokes MJ, Cooper RG, Edwards RHT (1988) Normal muscle strength and fatigability in patients with effort syndromes. Br Med J 297:1014–1017

    Google Scholar 

  • Vollestad NK, Sejersted OM, Bahr R, Woods JJ, Bigland-Ritchie R (1988) Motor drive and metabolic responses during repeated submaximal contractions in humans. J Appl Physiol 64:1421–1427

    Google Scholar 

  • Wagenmakers AJM, Beckers EJ, Brouns F, Kuipers H, Soeters PB, Van Der Vusse GJ, Saris WHM (1991a) Carbohydrate supplementation, glycogen depletion, and amino acid metabolism during exercise. Am J Physiol 260:E883–890

    Google Scholar 

  • Wagenmakers AJM, Smets K, Vandewalle L, Brouns F, Saris WHM (1991b) Deamination of branched-chain amino acids: a potential source of ammonia production during exercise (abstract). Med Sci Sports Exerc 23:S116

    Google Scholar 

  • Wolfe RR, Nadel ER, Shaw JHF, Stephenson LA, Wolfe MH (1987) Role of changes in insulin and glucagon in glucose homeostasis in exercise. J Clin Invest 77:900–907

    Google Scholar 

  • Wurtman RJ (1988) Effects of dietary amino acids, carbohydrates, and choline on neurotransmitter synthesis. Mt Sinai J Med (NY) 55:75–86

    Google Scholar 

  • Young SN (1986) The clinical psychopharmacology of tryptophan. In: Wurtman RJ, Wurtman JJ (eds) Nutrition and the brain, vol 7. Raven, NY, pp 49–88

    Google Scholar 

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Authors and Affiliations

  1. Department of Exercise Science, School of Public Health, University of South Carolina, 29208, Columbia, SC, USA

    J. Mark Davis, Stephen P. Bailey, Jeffrey A. Woods, Floyd J. Galiano, Marc T. Hamilton & William P. Bartoli

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  1. J. Mark Davis
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  2. Stephen P. Bailey
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  3. Jeffrey A. Woods
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  4. Floyd J. Galiano
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  5. Marc T. Hamilton
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  6. William P. Bartoli
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Davis, J.M., Bailey, S.P., Woods, J.A. et al. Effects of carbohydrate feedings on plasma free tryptophan and branched-chain amino acids during prolonged cycling. Europ. J. Appl. Physiol. 65, 513–519 (1992). https://doi.org/10.1007/BF00602357

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  • DOI: https://doi.org/10.1007/BF00602357

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