Summary
Pure solutions of TMV were irradiated with x-rays or ultraviolet light in order to study possible effects on the mutation rate of the virus. If no spontaneous mutations were observed in a particular virus preparation, no induced mutations resulted from irradiation. In preparations showing spontaneous mutations, the „rate of mutation“ was increased by irradiation only if the exposed material was compared with unirradiated virus diluted so as to give more lesions per leaf than the irradiated sample. The apparent mutation rate increased with dilution of active virus, whether this dilution was effected by irradiation or by the direct addition of phosphate buffer. Artificial unequal mixtures of two related virus strains, with either in the majority, were tested at different dilutions of the whole; without exception the symptoms of that partner present in minority became relatively more frequent with decreasing total concentration of the mixture.
The effects observed here, as well as those described byGowen (1941), can readily be explained on the basis of competition between related virus strains. The evidence does not support an hypothesis of mutagenic action of irradiation on purified TMV preparations.
Similar content being viewed by others
Literatur
Aach, H. G.: Im Druck, 1957.
Friedrich-Freksa, H., G. Melchers u.G. Schramm: Biologischer, chemischer und serologischer Vergleich zweier Parallelmutanten phytopathogener Viren mit ihren Ausgangsformen. Biol. Zbl.65, 187 (1946).
Gowen, T. W.: Mutation inDrosophila, bacteria and viruses. Cold Spring Harbor Symp. Quant. Biol.9, 187 (1941).
Harris, J. I., andC. A. Knight: Action of carboxypeptidase on tobacco mosaic virus. Nature (Lond.)170, 613 (1952).
Holmes, F. O.: Masked strain of tobacco mosaic virus. Phytopathology24, 845 (1934).
Comparisons of derivatives from distinctive strains of tobacco mosaic virus. Phytopathology26, 896 (1936).
Jensen, J. H.: Isolation of yellow mosaic viruses from plants infected with tobacco mosaic virus. Phythopathology23, 964 (1933).
Studies on the origin of yellow mosaic viruses. Phytopathology26, 266 (1936).
Studies on representative strains of tobacco mosaic virus. Phytopathology27, 69 (1937).
Knight, C. A.: The nature of some of the differences among strains of tobacco mosaic virus. J. of Biol. Chem.171, 298 (1947).
Koller, S.: Graphische Tafeln zur Beurteilung statistischer Zahlen. Dresden u. Leipzig: Theodor Steinkopff 1943.
Kramer, E.: Im Druck, 1957.
Lea, D. E.: Actions of radiations on living cells. Cambridge: University Press (1947).
McKinney, H. H.: Evidence of virus mutation in the common mosaic of tobacco. J. Agricult. Res.51, 951 (1935).
Virus mutation and the gene concept. J. Hered.28, 51 (1937)
Melchers, G.: Uber einige Mutationen des Tabakmosaikvirus und eine „Parallelmutation“ des Tomatenmosaikvirus. Naturwiss.30, 48 (1942).
Melchers, G., G. Schramm, H. Trurnit u.H. Friedrich-Freksa: Die biologische, chemische und elektronenmikroskopische Untersuchung eines Mosaikvirus aus Tomaten. Biol. Zbl.60, 524 (1942).
Melchers, G., u.W. Mundry: „Mutation“ und „Rückmutation“ beim Tabakmosaikvirus in Abhängigkeit von der Kulturtemperatur der Wirtspflanzen: Proc. 9. Internat. Congr. Genetics 1953. Caryologia (Pisa) Suppl.1954.
Mundry, W.: Die Abhängigkeit des Auftretens neuer Virusstämme von der Kulturtemperatur der Wirtspflanzen. Im Druck, 1957.
Norval, I. P.: Derivatives from an unusual strain of tobacco mosaic virus. Phytopathology28, 675 (1938).
Opatowsky, J.: On the mutation of tobacco mosaic virus. J. Gen. Physiol.33, 171 (1949).
Pfankuch, E. G., A. Kausche u.H. Stubbe: Über die Entstehung, die biologische und physikalisch-chemische Charakterisierung von Röntgen-und γ-Strahlen induzierten „Mutationen“ des Tabakmosaikvirusproteins. Biochem. Z.304, 238 (1940).
Pollard, E.: The physics of viruses. New York: Academic Press 1953.
Sadasivan, T. S.: A quantitative study of the interaction of viruses in plants. Ann. Appl. Biology27, 359 (1940).
Schramm, G.: Die Biochemie der Viren. Berlin: Springer 1954.
Schramm, G., u.G. Braunitzer: Prolin als Endgruppe des TMV. Z. Naturforsch. 8b, 61 (1953).
Schramm, G., u.L. Rebensburg: Zur vergleichenden Charakterisierung einiger Mutanten des TMV. Naturwiss.30, 48 (1942).
Sinnot, E., W. Dunn u.Th. Dobzhanski: Principles of genetics. New York: Mc Graw-Hill Book Comp. 1950.
Stanley, W. M., u.C. A. Knight: The chemical composition of strains of tobacco mosaic virus. Cold Spring Harbor Symp. Quantt. Biol..9, 225. (1941).
Timoféeff, N. W., K. G. Zimmer u.M. Delbrück: Über die Natur der Genmutation und der Genstruktur. Nachr. Ges. Wiss. Göttingen, Math.-physik. Kl., Fachgruppe VI, N. F.1, 189 (1935).
Troll, W.: Das Virusproblem in ontologischer Sicht. Wiesbaden: Franz Steiner 1951.
Weigle, J. J.: Induction of mutations in a bacterial virus. Proc. Nat. Acad. Sci. U. S. A.39, 628. Zit. nachW. Weidel, Fortschr. Bot.17, 878 (1953).
Weigle, J. J., u.R. Dulbecco: Induction of mutations in bacteriophage T3 by ultraviolet light. Experientia (Basel)9, 372 (1953). Zit. nachW. Weidel s. oben.
Author information
Authors and Affiliations
Additional information
Mit 1 Textabbildung
Ein Teil der hier veröffentlichten Ergebnisse ist Bestandteil einer Dissertation der math.-naturw. Fakultät der Univ. Tübingen 1954.
Rights and permissions
About this article
Cite this article
Mundry, KW. Zur Frage Des Einflusses Von Röntgen- Und Uv-Strahlen Auf Die Mutationsrate Des Tabakmosaikvirus Nach Bestrahlung Reiner Präparate. Z.Ver-erbungslehre 88, 115–127 (1957). https://doi.org/10.1007/BF00593656
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00593656